Non-invasive quantitative imaging of hepatocellular carcinoma growth in mice by micro-CT using liver-targeted iodinated nano-emulsions

Anton, Nicolas; Parlog, Alexandru; About, Ghina Bou; Attia, Mohamed F.; Wattenhofer‐Donzé, Marie; Jacobs, Hugues; Gonçalves, I. Sarmento; Robinet, Éric; Sorg, Tania; Vandamme, Thierry F.

doi:10.1038/s41598-017-14270-7

Cited by 23 publications

(22 citation statements)

References 34 publications

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“…In vivo non-invasive longitudinal monitoring in cancer mouse models is a powerful strategy to follow over time the progression of tumors in the same animal (Anton et al., 2017). Among available methods, X-ray micro-computed tomography (CT) has gained significant interest for a series of advantages it offers (Ashton et al., 2015, Martiniova et al., 2010, Schambach et al., 2010, Wathen et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography

et al. 2019

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SummaryComputed tomography is a powerful medical imaging modality for longitudinal studies in cancer to follow neoplasia progression and evaluate anticancer therapies. Here, we report the generation of a photon-counting micro-computed tomography (PC-CT) method based on hybrid pixel detectors with enhanced sensitivity and precision of tumor imaging. We then applied PC-CT for longitudinal imaging in a clinically relevant liver cancer model, the Alb-R26Met mice, and found a remarkable heterogeneity in the dynamics for tumors at the initiation phases. Instead, the growth curve of evolving tumors exhibited a comparable exponential growth, with a constant doubling time. Furthermore, longitudinal PC-CT imaging in mice treated with a combination of MEK and BCL-XL inhibitors revealed a drastic tumor regression accompanied by a striking remodeling of macrophages in the tumor microenvironment. Thus, PC-CT is a powerful system to detect cancer initiation and progression, and to monitor its evolution during treatment.

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Section: Introductionmentioning

confidence: 99%

Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography

et al. 2019

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“…A vast number of publications have investigated the use of polyester contrast agents for the enhancement of X‐ray scanning in both in vitro and in vivo . In 2015, a novel radiopaque 3D printable bone screw based on a poly‐ l ‐lactide composite, which contained 20% IONPs (Fe 3 O 4 ), was reported.…”

Section: Polyesters With Imaging Contrast Propertiesmentioning

confidence: 99%

“…Vandamme and co‐workers have been exploring different kinds of nontoxic, blood pool X‐ray imaging agents, two of which are polyester based formulations. One of the polyesters was synthesized via encapsulation of iodine into poly(ethylene glycol)‐poly(ε‐caprolactone) nanocapsules, which served as the X‐ray CT contrast agent, avoiding inorganic materials in the formulation .…”

Section: Polyesters With Imaging Contrast Propertiesmentioning

confidence: 99%

Recent Advances in Polyesters for Biomedical Imaging

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et al. 2018

Adv Healthcare Materials

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Several synthetic materials exhibiting contrast imaging properties have become vital to the field of biomedical imaging. Polymeric biomaterials and metals are commonly used imaging agents and can assist in the monitoring of therapy response, migration, degradation, changes in morphology, defects, and image-guided surgery. In comparison to metals, most bio and synthetic polymers lack inherent imaging properties. Polymeric biomaterials, specifically polyesters, have gained a considerable amount of attention due to their unique properties including biocompatibility, biodegradation, facile synthesis, and modification capability. Polyester implants and nanomaterials are available on the market or are in clinical trials for many applications including: dental implants, cranio-maxilofacial implants, soft tissue sutures and staples, abdominal wall repair, tendon and ligament reconstruction, fracture fixation devices, and coronary drug eluting stents. This review aims to provide a summary of the recent developments of polyesters with bioimaging contrast properties. The three main approaches to prepare bioimaging polyesters (coating, encapsulation, and functionalization) are discussed in depth. Furthermore, commonly used imaging modalities including X-ray computed tomography, magnetic resonance imaging, ultrasound, fluorescence, and radionucleotide polyester contrast agents are highlighted. In each section, examples of impactful bioimaging polyesters in the five major imaging modalities are evaluated.

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“…There are also practices that involve targeted imaging of tumors using small peptides and molecules. SCID mice have previously been used for such studies for ovarian ( 112 ), nasopharyngeal, breast ( 113 ), hepatic ( 114 ), lung cancer ( 115 ), and others. SCID mice are useful animal models for proof of concept studies that certain molecules and peptides can specifically bind to certain tumor types.…”

Section: Future Outlook On the Utilization Of Scid Pigs For Cancer Thmentioning

confidence: 99%

Development of Severe Combined Immunodeficient (SCID) Pig Models for Translational Cancer Modeling: Future Insights on How Humanized SCID Pigs Can Improve Preclinical Cancer Research

et al. 2018

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Within the last decade there have been several severe combined immunodeficient (SCID) pig models discovered or genetically engineered. The animals have mutations in ARTEMIS, IL2RG, or RAG1/2 genes, or combinations thereof, providing SCID pigs with NK cells, but deficient in T and B cells, or deficient in NK, T, and B cells for research studies. Biocontainment facilities and positive pressure isolators are developed to limit pathogen exposure and prolong the life of SCID pigs. Raising SCID pigs in such facilities allows for completion of long-term studies such as xenotransplantation of human cells. Ectopically injected human cancer cell lines develop into tumors in SCID pigs, thus providing a human-sized in vivo model for evaluating imaging methods to improve cancer detection and therapeutic research and development. Immunocompromised pigs have the potential to be immunologically humanized by xenotransplantation with human hematopoietic stem cells, peripheral blood leukocytes, or fetal tissue. These cells can be introduced through various routes including injection into fetal liver or the intraperitoneal (IP) space, or into piglets by intravenous, IP, and intraosseous administration. The development and maintenance of transplanted human immune cells would be initially (at least) dependent on immune signaling from swine cells. Compared to mice, swine share higher homology in immune related genes with humans. We hypothesize that the SCID pig may be able to support improved engraftment and differentiation of a wide range of human immune cells as compared to equivalent mouse models. Humanization of SCID pigs would thus provide a valuable model system for researchers to study interactions between human tumor and human immune cells. Additionally, as the SCID pig model is further developed, it may be possible to develop patient-derived xenograft models for individualized therapy and drug testing. We thus theorize that the individualized therapeutic approach would be significantly improved with a humanized SCID pig due to similarities in size, metabolism, and physiology. In all, porcine SCID models have significant potential as an excellent preclinical animal model for therapeutic testing.

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Non-invasive quantitative imaging of hepatocellular carcinoma growth in mice by micro-CT using liver-targeted iodinated nano-emulsions

Cited by 23 publications

References 34 publications

Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography

Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography

Recent Advances in Polyesters for Biomedical Imaging

Development of Severe Combined Immunodeficient (SCID) Pig Models for Translational Cancer Modeling: Future Insights on How Humanized SCID Pigs Can Improve Preclinical Cancer Research

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