Non-invasive skin biomarkers quantification of psoriasis and atopic dermatitis: Cytokines, antioxidants and psoriatic skin auto-fluorescence

Portugal-Cohen, Meital; Horev, Liran; Ruffer, Claas; Schlippe, Gerrit; Voss, Werner; Ma’or, Ze’evi; Oron, Miriam; Soroka, Yoram; Frušić-Zlotkin, Marina; Milner, Yoram; Kohen, Ron

doi:10.1016/j.biopha.2011.12.009

Cited by 49 publications

(65 citation statements)

References 57 publications

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“…However an increase of secreted IL-1α in lesional skin of psoriasis patients was observed [101]. After acute permeability barrier disruption, an increase in the epidermal expression of IL-1α and IL-1β occurs, which might have protective function [102].…”

Section: Cytokine Familiesmentioning

confidence: 99%

Cytokines and the Skin Barrier

Hänel

Cornélissen

Lüscher

et al. 2013

IJMS

248

195

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The skin is the largest organ of the human body and builds a barrier to protect us from the harmful environment and also from unregulated loss of water. Keratinocytes form the skin barrier by undergoing a highly complex differentiation process that involves changing their morphology and structural integrity, a process referred to as cornification. Alterations in the epidermal cornification process affect the formation of the skin barrier. Typically, this results in a disturbed barrier, which allows the entry of substances into the skin that are immunologically reactive. This contributes to and promotes inflammatory processes in the skin but also affects other organs. In many common skin diseases, including atopic dermatitis and psoriasis, a defect in the formation of the skin barrier is observed. In these diseases the cytokine composition within the skin is different compared to normal human skin. This is the result of resident skin cells that produce cytokines, but also because additional immune cells are recruited. Many of the cytokines found in defective skin are able to influence various processes of differentiation and cornification. Here we summarize the current knowledge on cytokines and their functions in healthy skin and their contributions to inflammatory skin diseases.

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Section: Cytokine Familiesmentioning

confidence: 99%

Cytokines and the Skin Barrier

Hänel

Cornélissen

Lüscher

et al. 2013

IJMS

248

195

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“…Sweat and interstitial fluid are most often referred to as analytes . In addition, some studies have shown that the stratum corneum of the human skin is a biochemically active and informative object of research ; the prospects of using washings from the human skin as an analyte have been noted .…”

Section: Introductionmentioning

confidence: 99%

“…A non‐invasive approach to the determination of skin antioxidants, described in the works of Kohen et al. , is based on the skin surface wash sampling and its analysis with the use of high‐performance liquid chromatography with electrochemical detection and oxygen radical absorbance capacity assay (total scavenging capacity of hydrophilic antioxidants determination). However, the proposed approaches are not express, and require expensive equipment and materials.…”

Section: Introductionmentioning

confidence: 99%

Optimized Potentiometric Assay for Non‐invasive Investigation of Skin Antioxidant Activity

2018

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The paper presents a new variant of non‐invasive potentiometric method for determination of human skin antioxidant activity (AOA). The use of a porous cellulose acetate membrane as the carrier for reagent solution (potassium hexacyanoferrate (III)) is presented. The increase of reagent shelf life, improvement the reproducibility of the results (during the day Sr≤0.08, interday Sr≤0.13) in comparison with previously described variant is demonstrated. It was shown that the components of the skin hydrolipid mantle and pH do not affect the potentiometric signal. The values of skin AOA determined by the developed method lie in the range 20–4000 μM‐eq. Recovery is Raverage=99 %. The skin of healthy people was studied by the developed method. The obtained results indicate the prospects of using a new non‐invasive approach in the study of the oxidation‐reduction balance of human skin.

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“…For instance, the optical density of the OCT images has been shown to correlate with systemic sclerosis 23 and average skin attenuation has been explored as a predictor of solar elastosis score 24 , while psoriasis has been shown to affect skin fluorescence 25 . Although the mechanism is not completely clear, polysaccharides have been shown to exert antitumor action 26 , thus the authors hypothesize the value of using their distribution as a marker of tumor prognosis.…”

Section: Resultsmentioning

confidence: 99%

Ex-vivo multi-modal microscopy of healthy skin

et al. 2014

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The thorough characterization of skin samples is a critical step in investigating dermatological diseases. The combination of depth-sensitive anatomical imaging with molecular imaging has the potential to provide vast information about the skin. In this proof-of-concept work we present high-resolution mosaic images of skin biopsies using Optical Coherence Tomography (OCT) manually co-registered with standard microscopy, bi-dimensional Raman spectral mapping and fluorescence imaging.A human breast skin sample, embedded in paraffin, was imaged with a swept-source OCT system at 1310 nm. Individual OCT volumes were acquired in fully automated fashion in order to obtain a large field-of-view at high resolution (~10μm). Based on anatomical features, the other three modalities were manually co-registered to the projected OCT volume, using an affine transformation.A drawback is the manual co-registration, which may limit the utility of this method. However, the results indicate that multiple imaging modalities provide complementary information about the sample.This pilot study suggests that multi-modal microscopy may be a valuable tool in the characterization of skin biopsies.

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Non-invasive skin biomarkers quantification of psoriasis and atopic dermatitis: Cytokines, antioxidants and psoriatic skin auto-fluorescence

Cited by 49 publications

References 57 publications

Cytokines and the Skin Barrier

Cytokines and the Skin Barrier

Optimized Potentiometric Assay for Non‐invasive Investigation of Skin Antioxidant Activity

Ex-vivo multi-modal microscopy of healthy skin

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