Non-ionic surfactant formulation sequentially enhances the enzymatic hydrolysis of cellulignin from sugarcane bagasse and the production of Monascus ruber biopigments

Sánchez-Muñoz, Salvador; Balbino, Thércia Rocha; Hilares, Ruly Terán; Mier-Alba, Edith; Barbosa, Fernanda Gonçalves; Balagurusamy, Nagamani; Santos, Júlio César dos; Silva, S.S. da

doi:10.1016/j.biortech.2022.127781

Cited by 8 publications

(7 citation statements)

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Section: Additives As Emerging Tools To Enhance Enzymatic Hydrolysismentioning

confidence: 99%

“…To gain more insights, only a few studies are found regarding the use of complex hydrolysis formulations [ 4 , 84 ]. More precisely, most works are conducted either solely with surfactant mixtures or non-catalytic proteins [ 6 , 34 , 84 , 85 ]. As yet, there have been few studies conducted with biosurfactants and even with the combination of surfactant/biosurfactant/non-catalytic proteins [ 41 , 51 ].…”

Section: Additives As Emerging Tools To Enhance Enzymatic Hydrolysismentioning

confidence: 99%

“…Zheng et al [ 59 ] evaluated the impact of Tween 20, Tween 80, and BSA on enzyme loading and achieved the most effective results with Tween 20, with a two-fold reduction in enzyme loading. Similarly, Sanchez-Muñoz et al [ 84 ] achieve a four-fold reduction using Tween 20 and PEG400 in the hydrolysis of sugarcane bagasse. In addition, more than a six-fold reduction in enzyme loading was achieved by Lu et al [ 31 ] with a higher glucan conversion after 36 h of hydrolysis using PEG3000.…”

Section: Potential Enzyme Cost Reduction and Future Perspectives In T...mentioning

confidence: 99%

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Surfactants, Biosurfactants, and Non-Catalytic Proteins as Key Molecules to Enhance Enzymatic Hydrolysis of Lignocellulosic Biomass

et al. 2022

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Lignocellulosic biomass (LCB) has remained a latent alternative resource to be the main substitute for oil and its derivatives in a biorefinery concept. However, its complex structure and the underdeveloped technologies for its large-scale processing keep it in a state of constant study trying to establish a consolidated process. In intensive processes, enzymes have been shown to be important molecules for the fractionation and conversion of LCB into biofuels and high-value-added molecules. However, operational challenges must be overcome before enzyme technology can be the main resource for obtaining second-generation sugars. The use of additives is shown to be a suitable strategy to improve the saccharification process. This review describes the mechanisms, roles, and effects of using additives, such as surfactants, biosurfactants, and non-catalytic proteins, separately and integrated into the enzymatic hydrolysis process of lignocellulosic biomass. In doing so, it provides a technical background in which operational biomass processing hurdles such as solids and enzymatic loadings, pretreatment burdens, and the unproductive adsorption phenomenon can be addressed.

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Section: Additives As Emerging Tools To Enhance Enzymatic Hydrolysismentioning

confidence: 99%

Section: Additives As Emerging Tools To Enhance Enzymatic Hydrolysismentioning

confidence: 99%

Section: Potential Enzyme Cost Reduction and Future Perspectives In T...mentioning

confidence: 99%

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Surfactants, Biosurfactants, and Non-Catalytic Proteins as Key Molecules to Enhance Enzymatic Hydrolysis of Lignocellulosic Biomass

et al. 2022

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“…The use of lignin-blocking additives has emerged as a pivotal strategy to boost the release of fermentable sugar and reduce cellulase dosage by significantly mitigating the nonproductive adsorption of cellulase onto residual lignin within pretreated materials. − The lignin-blocking additives used in enzymatic hydrolysis encompass noncatalytic proteins, including bovine serum albumin (BSA), soy protein, and sodium caseinate, − as well as nonionic and ionic surfactants/polymers, exemplified by Tween, PEG, and lignin-based surfactants. − A study into the efficacy of nonionic surfactants, specifically a formulation composed of Tween 20, Triton X-100, and PEG400, unveiled their capacity to augment the enzymatic hydrolysis of sugar cane bagasse. This formulation afforded final yields of total reducing sugars from 32 to 63% at a cellulase loading of 10 FPU/g of biomass . PEG addition in saccharification of hydrothermally pretreated wheat straw led to an increase in the carbohydrate conversion from 29.8 to 43.3% at an enzyme dosage of 4 FPU/g substrate .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Copolymer with Tunable Cation Content as an Efficient Promoter in Enzymatic Saccharification of Lignocellulose

Xu,

Zhang,

Liao

et al. 2023

ACS Appl. Polym. Mater.

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Nonproductive adsorption of cellulase is widely considered as the major roadblock to enzymatically hydrolyzing lignocellulosic biomass. Cationic additives present a promising avenue for mitigating the issue by effectively obstructing cellulase adsorption onto the lignin surface through enhanced electrostatic interactions with the negatively charged lignin, in contrast to nonionic additives. However, excessive positive charges carried by ionic additives can potentially hamper enzyme activity. To reconcile the dilemma, we synthesized a copolymer featuring modulatable cationic content via control over the ratio of nonionic and cationic units within the polymer backbone. Upon addition of 1.0 g/L of the resultant cationic copolymer, a notable enhancement in glucose release from 39 to 88% was observed, even at a low cellulase dosage of 5 FPU/g glucan. Remarkably, these cationic copolymers exhibit versatile applicability across diverse enzyme preparations, acid-treated lignocellulosic substrates, and highly solid saccharification systems. Furthermore, an enzyme saving of up to 80% was achieved. Mechanistic studies illuminated that this copolymer can not only attenuate the occurrence of irreversible binding between lignin and cellulase but also exert a favorable influence on enzyme activity. The adaptability of the copolymer’s cationic content endows it with the status of a promising and effective lignin-blocking additive, holding considerable potential for advancing the economic feasibility of biofuel production.

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Sustainability analysis of biorefineries applying biotechnological routes to convert bagasse from non-centrifugal sugar production for rural economic development in Colombia

Ortiz-Sanchez,

Piedrahita-Rodríguez,

Solarte-Toro

et al. 2024

Biomass Conv. Bioref.

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Panela is the second most important rural agro-industry after coffee in Colombia. This agro-industry produces a large amount of bagasse from non-centrifugal sugar (B-NCSP) without valorization. B-NCSP is used in combustion boilers in panela production, causing greenhouse gas emissions and health problems. This research aims to compare the sustainability of two B-NCSP biorefineries considering the current residue use. The sustainability of the biorefinery scenarios was analyzed to promote rural economic development in Colombia. In both biorefinery scenarios, biogas was evaluated as an energy vector to meet the energy demand of panela production. Two biorefinery scenarios were considered: (i) saccharification, bioethanol, and biogas production and (ii) acid pretreatment, saccharification, xylitol, bioethanol, and biogas production. Experimental data of the pretreatment, saccharification, and anaerobic digestion stages were used as input information in simulations. Technical, economic, environmental, and social metrics were used to estimate a sustainability index. The experimental glucose yield in scenario 2 was 0.42 ± 0.03 (31.52 ± 2.36 g/L) g/g B-NCSP, 1.8 times greater than scenario 1. The biogas yield in scenario 2 was 504.23 ± 21.68 (71.16% CH4) L/kg B-NCSP, 3.3 times greater than scenario 1. Scenario 1 was unfeasible at different scales. Xylitol production helps to improve the economic feasibility in scenario 2 since the payback period was 15 years. This scenario can create 36 job positions with a wage 18% higher than the minimum wage in Colombia (highest sustainability index of 72.44%). Finally, biogas produced in scenario 2 supplies 62.76% of the energy demand needed in the panela production process, reducing the environmental impact.

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Non-ionic surfactant formulation sequentially enhances the enzymatic hydrolysis of cellulignin from sugarcane bagasse and the production of Monascus ruber biopigments

Cited by 8 publications

References 50 publications

Surfactants, Biosurfactants, and Non-Catalytic Proteins as Key Molecules to Enhance Enzymatic Hydrolysis of Lignocellulosic Biomass

Surfactants, Biosurfactants, and Non-Catalytic Proteins as Key Molecules to Enhance Enzymatic Hydrolysis of Lignocellulosic Biomass

Copolymer with Tunable Cation Content as an Efficient Promoter in Enzymatic Saccharification of Lignocellulose

Sustainability analysis of biorefineries applying biotechnological routes to convert bagasse from non-centrifugal sugar production for rural economic development in Colombia

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