Non-isotopic receptor assay for benzodiazepines using a biotin-labeled ligand and biotin-immobilized microtiter plate

Tanaka, Shunitz; Takeuchi, Toshio; Rechnitz, G. A.

doi:10.1016/0021-9673(92)80143-i

Cited by 12 publications

(5 citation statements)

References 12 publications

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“…The assays make use of either a ligand coupled to an enzyme [42] or measure receptor-ligand binding based on enzyme activity via an indirect route [43][44][45][46][47]. An example of the latter is described by Mahoney [44] for the platelet-derived growth factor receptor (PDGF-R).…”

Section: Heterogeneous Non-radioactive Receptor Assaymentioning

confidence: 99%

Receptor–ligand binding assays: Technologies and Applications

Jong

Uges

Franke

et al. 2005

Journal of Chromatography B

173

129

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Section: Heterogeneous Non-radioactive Receptor Assaymentioning

confidence: 99%

Receptor–ligand binding assays: Technologies and Applications

Jong

Uges

Franke

et al. 2005

Journal of Chromatography B

173

129

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“…Details concerning the procedure for the enzyme binding assay using a biotinylated ligand and the biotin-immobilized microtiter plate (biotin plate) were described in a previous report. 25 The biotin plate was prepared as follows: 0.002% biotin-BSA phosphate buffer solution (pH 7.0) was added to the wells of the microtiter plate (Wako Pure Chem. Co.) and incubated overnight at 4 °C.…”

Section: Procedures Of the Enzyme Binding Assay Using Biotinylated Es...mentioning

confidence: 99%

“…In a previous study, we have described the receptor binding assay for benzodiazepine drugs using this principle. 25 The competitive binding curves for 17b-estradiol, 17b-estradiol-6-one and estradiol-SH obtained by this enzyme binding assay are shown in Fig. 6.…”

Section: Enzyme Binding Assay Using Biotinylated Estradiolmentioning

confidence: 99%

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Electrochemical immunoassay at a 17β-estradiol self-assembled monolayer electrode using a redox marker

Kuramitz

Matsuda

Thomas

et al. 2003

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A simple electrochemical immunoassay was demonstrated using a 17beta-estradiol modified electrode. 17beta-estradiol was immobilized on the gold electrode surface with a self-assembly technique. The specific binding between estradiol antibody and 17beta-estradiol on the electrode surface was evaluated by monitoring the change in the electrode response with three hydrophilic redox markers. The decrease in the electrode response for the redox marker was observed, when the antibody was bound to the estradiol self-assembled monolayer (SAM) electrode surface. The change in the electrode response of the redox marker is attributed to the steric hindrance between the antibody on the electrode surface and the redox marker. The relative standard deviation at 30 microg ml(-1) estradiol antibody was 4.1% (n = 3). The competitive reaction between the antigen in the solution and 17beta-estradiol immobilized on the electrode surface for the limited binding sites on the antibody produced an increase in the electrode response with hydroquinone as the marker. The binding affinity of three antigens including 17beta-estradiol to the estradiol antibody was evaluated. Furthermore, the result obtained from this method was compared with the previously reported enzyme binding assay using the biotinylated estradiol and the biotin-immobilized microtiter plate.

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“…24 IC 50 was the concentration of the displacing ligand required to inhibit 50% of the probe ligand binding. As a result, IC 50 of the LB to avidin (3.2×10 -10 M) was similar to that of biotin (7.9×10 -10 M).…”

Section: Change In the Electrode Response Of Lb By Interaction With Amentioning

confidence: 99%

Electrochemical Detection of Biotin Using an Interaction between Avidin and Biotin Labeled with Ferrocene at a Perfluorosulfonated Ionomer Modified Electrode

et al. 1999

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The electrochemical behavior of biotin labeled with ferrocene was investigated at a perfluorosulfonated ionomer (Nafion ® ) film-coated glassy carbon electrode (GCE). The electrode response of the labeled biotin (LB) was improved by about fifty-fold in sensitivity by using a Nafion ® -coated GCE, compared with a plain GCE. It was found by a solid-phase avidin-biotin binding assay that the labeling with ferrocene hardly affected the affinity of a biotin moiety in the labeled biotin to avidin. The avidin-biotin interaction was estimated from the change of the electrode response caused by the formation of a complex with avidin and the labeled biotin. Furthermore, the detection of biotin was performed by a competitive reaction between the labeled biotin and unlabeled biotin for the limited binding sites of avidin.

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Non-isotopic receptor assay for benzodiazepines using a biotin-labeled ligand and biotin-immobilized microtiter plate

Cited by 12 publications

References 12 publications

Receptor–ligand binding assays: Technologies and Applications

Receptor–ligand binding assays: Technologies and Applications

Electrochemical immunoassay at a 17β-estradiol self-assembled monolayer electrode using a redox marker

Electrochemical Detection of Biotin Using an Interaction between Avidin and Biotin Labeled with Ferrocene at a Perfluorosulfonated Ionomer Modified Electrode

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