Non-labeled virus detection using inverted triangular Au nano-cavities arrayed as SERS-active substrate

Chang, Chia Wei; Liao, Jiunn Der; Shiau, Ai‐Li; Yao, Chih Kai

doi:10.1016/j.snb.2011.04.006

Cited by 41 publications

(31 citation statements)

References 53 publications

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“…194 Also, inverted triangular Au nanocavities with various indentation depths and tip-to-tip displacements were well arrayed as a substrate for qualitative virus detection. 195 Through the induction of the electromagnetic effect by the substrate, the virus can be distinguished from the amino acids on its surface. The detectable concentration for encephalomyocarditis virus or adenovirus was 10 6 PFU.mL −1 and for influenza virus was 10 4 PFU.mL −1 .…”

Section: Surface-enhanced Raman Scatteringmentioning

confidence: 99%

“…The detectable concentration for encephalomyocarditis virus or adenovirus was 10 6 PFU.mL −1 and for influenza virus was 10 4 PFU.mL −1 . 195 A highly sensitive immunoassay utilizing SERS has been developed with a new Raman reporter and a unique SERS-active substrate incorporated into a microfluidic device for detection of HBV antigen from human blood.…”

Section: Surface-enhanced Raman Scatteringmentioning

confidence: 99%

See 1 more Smart Citation

Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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Section: Surface-enhanced Raman Scatteringmentioning

confidence: 99%

Section: Surface-enhanced Raman Scatteringmentioning

confidence: 99%

Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

View full text Add to dashboard Cite

show abstract

“…On the other hand, low throughput is a critical factor impeding the development of AFM tip-based nanofabrication methods. It has been demonstrated that the AFM tip-based nanoindentation process is time-consuming for obtaining large-scale nanodot structures [ 19 ]. To solve this problem, Vettiger et al presented the concept of “Millipede,” which employs large arrays of micro-cantilevers operating in parallel to achieve ultra-high-density machining capability [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Nanoscale Pits with High Throughput on Polymer Thin Film Using AFM Tip-Based Dynamic Plowing Lithography

Geng

Yan

et al. 2017

Nanoscale Res Lett

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We show that an atomic force microscope (AFM) tip-based dynamic plowing lithography (DPL) approach can be used to fabricate nanoscale pits with high throughput. The method relies on scratching with a relatively large speed over a sample surface in tapping mode, which is responsible for the separation distance of adjacent pits. Scratching tests are carried out on a poly(methyl methacrylate) (PMMA) thin film using a diamond-like carbon coating tip. Results show that 100 μm/s is the critical value of the scratching speed. When the scratching speed is greater than 100 μm/s, pit structures can be generated. In contrast, nanogrooves can be formed with speeds less than the critical value. Because of the difficulty of breaking the molecular chain of glass-state polymer with an applied high-frequency load and low-energy dissipation in one interaction of the tip and the sample, one pit requires 65–80 penetrations to be achieved. Subsequently, the forming process of the pit is analyzed in detail, including three phases: elastic deformation, plastic deformation, and climbing over the pile-up. In particular, 4800–5800 pits can be obtained in 1 s using this proposed method. Both experiments and theoretical analysis are presented that fully determine the potential of this proposed method to fabricate pits efficiently.

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“…SERS was also demonstrated in the presence of a roughened metal surface, without the need for a metal particle suspension [ 13 ]. Virus detection has been demonstrated on SERS-active substrates fabricated by various methods, such as nano-identation [ 14 ], oblique angle deposition [ 15 ], or focused ion beam lithography [ 16 , 17 ]. Although they can potentially provide label-free detection of viruses from solutions under certain conditions, these SERS substrate nanofabrication methods are currently non-trivial; hence, their integration with the relatively simple photolithographic process of microelectrode deposition that provides accelerated target capture from solution may substantially increase the manufacturing complexity of a biosensor chip.…”

Section: Introductionmentioning

confidence: 99%

Accelerated Detection of Viral Particles by Combining AC Electric Field Effects and Micro-Raman Spectroscopy

Tomkins

Liao

Docoslis

2015

Sensors

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A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the “fingerprinting” capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses.

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Non-labeled virus detection using inverted triangular Au nano-cavities arrayed as SERS-active substrate

Cited by 41 publications

References 53 publications

Nanoscale virus biosensors: state of the art

Nanoscale virus biosensors: state of the art

Fabrication of Nanoscale Pits with High Throughput on Polymer Thin Film Using AFM Tip-Based Dynamic Plowing Lithography

Accelerated Detection of Viral Particles by Combining AC Electric Field Effects and Micro-Raman Spectroscopy

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