Non-muscle myosin IIA is post-translationally modified by interferon-stimulated gene 15 in breast cancer cells

Cruz-Ramos, Eduardo; Macı́as-Silva, Marina; Sandoval-Hernández, Antonio; Tecalco-Cruz, Ángeles C.

doi:10.1016/j.biocel.2018.12.002

Cited by 10 publications

(9 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The association between high expression of ISG15 and nodal status in the METABRIC cohort also supports its ability to contribute in BC invasion and metastasis. These results are in accordance with several previous studies, which demonstrated that ISG15 is significantly associated with cancer progression [17,[43][44][45][46]. Although in our study some variation in the association between ISG15 expression and clinicopathological variables such as nodal status, this can be attributed to the difference in the nature of cohort, convoluted post-transcriptional mechanisms or perhaps due to the substantial differences in in vivo half-lives of proteins [48,49].…”

Section: Discussionsupporting

confidence: 93%

“…LVI is an essential process in the metastatic cascade that needs further studies. ISG15 expression is upregulated in various cancers, including breast [43], hepatocellular [44], lung [45], prostate [46] and bladder [47] cancers. However, the association between ISG15 and BC progression, particularly its role in LVI, yet remains to be defined.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The prognostic significance of interferon-stimulated gene 15 (ISG15) in invasive breast cancer

Kariri

Alsaleem

Joseph

et al. 2020

Breast Cancer Res Treat

View full text Add to dashboard Cite

Background Lymphovascular invasion (LVI) is a prognostic factor in early-stage invasive breast cancer (BC). Through bioinformatics, data analyses of multiple BC cohorts revealed the positive association between interferon-stimulated gene 15 (ISG15) LVI status. Thus, we explored the prognostic significance of ISG15 in BC. Methods The prognostic significance of ISG15 mRNA was assessed in METABRIC (n = 1980), TCGA (n = 854) and Kaplan–Meier Plotter (n = 3951). ISG15 protein was evaluated using immunohistochemistry (n = 859) in early-stage invasive BC patients with long-term follow-up. The associations between ISG15 expression and clinicopathological features, expression of immune cell markers and patient outcome data were evaluated. Results High mRNA and protein ISG15 expression were associated with LVI, higher histological grade, larger tumour size, hormonal receptor negativity, HER2 positivity, p53 and Ki67. High ISG15 protein expression was associated with HER2-enriched BC subtypes and immune markers (CD8, FOXP3 and CD68). High ISG15 mRNA and ISG15 expressions were associated with poor patient outcome. Cox proportional multivariate analysis revealed that the elevated ISG15 expression was an independent prognostic factor of shorter BC-specific survival. Conclusion This study provides evidence for the role of ISG15 in LVI development and BC prognosis. Further functional studies in BC are warranted to evaluate the therapeutic potential of ISG15.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

The prognostic significance of interferon-stimulated gene 15 (ISG15) in invasive breast cancer

Kariri

Alsaleem

Joseph

et al. 2020

Breast Cancer Res Treat

View full text Add to dashboard Cite

show abstract

“…Thus, we identified 25 RBPs (Fig. 6B): 5 never studied in cancer (CSTF2T, SRRM1, CPSF7, EIF4G3, and ELOA), 6 associated with other cancer types (TNRC6B 62 , SEC31A 63 , SPTAN1 64 , MARK2 65 , TNRC6A 66 , and PMS1 67 ), 7 associated with BC (CASC3 68 , HNRNPF 69 , MYH9 70 TLN1 71 , DDX5 72 , TAF15 73 , and EIF4G1 74 ), 3 already described as tumor suppressors in BC (CTNND1 23 , LIMA1 24 , AATF 25 ), and 4 identified as tumor suppressors in other cancer types (MEX3C 75 , UPF1 76 , CNOT1 77 , and TNKS1BP1 78 ).…”

Section: Resultsmentioning

confidence: 99%

In silico analyses reveal new putative Breast Cancer RNA-binding proteins

Guerrero

López‐Cortés

García-Cárdenas

et al. 2020

Preprint

View full text Add to dashboard Cite

Breast cancer (BC) is the leading cause of cancer-associated death among women worldwide. Despite treatment efforts, advanced BC with distant organ metastases is considered incurable. A better understanding of BC molecular processes is therefore of great interest to identify new therapeutic targets. Although large-scale efforts, such as The Cancer Genome Atlas (TCGA), have completely redefined cancer drug development, diagnosis, and treatment, additional key aspects of tumor biology remain to be discovered. In that respect, post-transcriptional regulation of tumorigenesis represents an understudied aspect of cancer research. As key regulators of this process, RNA-binding proteins (RBPs) are emerging as critical modulators of tumorigenesis but only few have defined roles in BC. To unravel new putative BC RBPs, we have performed in silico analyses of all human RBPs in three major cancer databases (TCGA-Breast Invasive Carcinoma, the Human Protein Atlas, and the Cancer Dependency Map project) along with complementary bioinformatics resources (STRING protein-protein interactions and the Network of Cancer Genes 6.0). Thus, we have identified six putative BC progressors (MRPL13, SCAMP3, CDC5L, DARS2, PUF60, and PLEC), and five BC suppressors RBPs (SUPT6H, MEX3C, UPF1, CNOT1, and TNKS1BP1). These proteins have never been studied in BC but show similar cancer-associated features than well-known BC proteins. Further research should focus on the mechanisms by which these proteins promote or suppress breast tumorigenesis, holding the promise of new therapeutic pathways along with novel drug development strategies.

show abstract

“…ISG15 can disrupt cytoskeletal architecture and promote motility in human breast cancer cells [ 56 , 57 ]. Cytoskeleton remodelling and associated signalling was found to be regulated by the isgylation of IQGAP1 [ 58 , 59 ], non-muscle myosin IIA [ 60 ] and filamin B [ 61 ]. The activation of Stat1 by focal adhesion kinase FAK is involved in integrin-mediated cell migration and adhesion [ 62 , 63 ].…”

Section: Discussionmentioning

confidence: 99%

Sustained Inflammatory Signalling through Stat1/Stat2/IRF9 Is Associated with Amoeboid Phenotype of Melanoma Cells

Gandalovičová

Šůchová

Čermák

et al. 2020

Cancers

View full text Add to dashboard Cite

The invasive behaviour of cancer cells underlies metastatic dissemination; however, due to the large plasticity of invasion modes, it is challenging to target. It is now widely accepted that various secreted cytokines modulate the tumour microenvironment and pro-inflammatory signalling can promote tumour progression. Here, we report that cells after mesenchymal–amoeboid transition show the increased expression of genes associated with the type I interferon response. Moreover, the sustained activation of type I interferon signalling in response to IFNβ mediated by the Stat1/Stat2/IRF9 complex enhances the round amoeboid phenotype in melanoma cells, whereas its downregulation by various approaches promotes the mesenchymal invasive phenotype. Overall, we demonstrate that interferon signalling is associated with the amoeboid phenotype of cancer cells and suggest a novel role of IFNβ in promoting cancer invasion plasticity, aside from its known role as a tumour suppressor.

show abstract

Non-muscle myosin IIA is post-translationally modified by interferon-stimulated gene 15 in breast cancer cells

Cited by 10 publications

References 47 publications

The prognostic significance of interferon-stimulated gene 15 (ISG15) in invasive breast cancer

The prognostic significance of interferon-stimulated gene 15 (ISG15) in invasive breast cancer

In silico analyses reveal new putative Breast Cancer RNA-binding proteins

Sustained Inflammatory Signalling through Stat1/Stat2/IRF9 Is Associated with Amoeboid Phenotype of Melanoma Cells

Contact Info

Product

Resources

About