In this paper, a two-dimensional numerical investigation is carried out to understand the effects of opposing thermal buoyancy and Prandtl number on fluid flow and mixed convection heat transfer characteristics of symmetrically and asymmetrically confined cylinder submerged in Newtonian fluid. The detailed flow and temperature field are illustrated in term of streamlines and isotherm contours to interpret the flow and thermal transport visualization. The numerical results are presented and discussed for the range of conditions as: Ri = 0 to -4, Pr = 0.7 to 50, eccentricity factor ε = 0 to 0.7 at Re = 40 and for a fixed blockage parameter B = 0.2. The effect of opposing buoyancy is brought about by varying Richardson numbers. The overall drag coefficient and average Nusselt number are computed to elucidate the role of Prandt number, eccentricity factor and Richardson number on the flow and heat transfer. At ε = 0, it is found that, when the buoyancy is opposed the flow becomes asymmetrically and some rotating zones appear under and above the cylinder. Moreover, the eccentricity factor has a tendency to decrease the rotating regions and to increase the heat transfer rate. For example an increase in eccentricity factor from 0 to 0.6 increases Nu by 77% at Ri = 4.