The replacement of historical load-bearing wooden elements made from not commonly used species such as Populus tremula L. presents significant challenges. As these species are seldomly used in modern construction, a knowledge gap exists regarding their implementation in accordance with current building codes. This study investigates the mechanical properties of European aspen (Populus tremula L.) from central Slovakia as a potential replacement for historical structures. Notably, poplar species, including European aspen, have historically been utilized for construction across various landscapes in Europe. We conducted experimental testing on visually graded aspen timber to determine the dynamic modulus of elasticity (MOEdyn,ultr), modulus of elasticity (MOE), modulus of rupture (MOR), and density. The results were analyzed and compared to established standards for structural timber. Notably, the 5th percentileof the strength distribution (f0.05) was determined to be 28.78 MPa, while the characteristic strength (fk) was 26.23 MPa, and the modulus of elasticity (Eg12) was 13.60 MPa. The correlation between MOR and dynamic MOE facilitated the determination of MOR by non-destructive testing (NDT) using the Sylvatest Duo®. This simple linear model could grade 49% of boards into the higher strength class C30. The additional parameters and their interactions in multiregresssion models improved the predictability of the bending strength of aspen. The advanced model graded 68% of boards into C30. These characteristics, along with aspen’s growth potential, make it a promising candidate for replacing damaged structural elements in historical constructions. Our findings contribute to the understanding of the potential of European aspen as a structural timber, highlighting its viability as a fast-growing hardwood species.