Non-random distribution of deletion endpoints in the gal operon of E. coli

Pfeifer, Dietrich; Hirsch, Heinz‐Josef; Bergmann, Dirk; Hamlaoui, M.

doi:10.1007/bf00269393

Cited by 19 publications

(5 citation statements)

References 18 publications

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“…This may represent a deletion endpoint 'hot-spot'. Such clustering of deletion endpoints has been observed in the gal region of E. coli and has been shown to occur as a result both of heat induction of A (Pfeifer et at. 1974) and of /(Sfl-mediated deletion formation (Reif & Saedler, 1975).…”

Section: Discussionmentioning

confidence: 77%

Deletion mapping of theniiAniaD gene region ofAspergillus nidulans

Tomsett

Cove

1979

Genet. Res.

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SUMMARYThe genetic fine-structure of theniiAniaD gene region ofAspergillus nidulanshas been studied using deletion mapping. Deletions were identified asniiAniaD double mutants and comprised 1% of spontaneous chlorate-resistant mutants. All such double mutants were shown to involve deletions and their frequency was not increased by mutagenic treatment with eitherN-methyl-N′-nitro-N-nitrosoguanidine or with ultraviolet light. Deletion maps of theniaD andniiA genes have been constructed. A further class of mutation was also mapped using the deletions. Thesecrnmutations, which affect a gene whose function is as yet unknown, map on the centromere proximal side ofniiA. This analysis of a eukaryote gene cluster will provide a framework upon which to base studies of the regulation of the nitrate assimilation pathway.

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Section: Discussionmentioning

confidence: 77%

Deletion mapping of theniiAniaD gene region ofAspergillus nidulans

Tomsett

Cove

1979

Genet. Res.

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“…would be expected (Pfeifer et al, 1974), to arise from specific processes. In the case of spontaneously generated deletions, there appears to be a 'hotspot' beyond purD since the majority of deletions have one endpoint that removes this locus (see also Linn et al, 1979).…”

Section: Resultsmentioning

confidence: 99%

Deletion Analysis of Essential Genes of Escherichia coli: Investigation of the btuB-rpoBC Interval

McKEE

Glass

1982

Microbiology

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A method is described for deletion mapping of essential genes in Escherichia coli. It involves the isolation of secondary-site insertions of lambda cI857plac5 into an F' plasmid. The transposed lacZ gene is useful both for the ready screening of plasmid-phage cointegrates and for rapid analysis of deletions that extend from the site of phage integration into the bacterial genes carried on the substituted plasmid. Such deletions may be used to 'hook-up' bacterial cistrons to the powerful lac promoter. We report the application of this technique to the study of the btuB-rpoBC interval, a cluster of genes encoding components of the transcription-translation apparatus.

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“…Moreover, the rearrangements involved in the construction of potential low frequency transducing strains are results of illegitimate recombination events (11). These occur rarely and very likely not with equal probability at all sites on participating DNA molecules (27,32). Hence, for any particular gene, not all approaches may yield transducing phages at a detectable frequency.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli

Steege¹,

Low²

1975

J Bacteriol

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Specialized lambda transducing phages for the sul + (supD-) amber suppresssor in Escherichia coli K-12 have been isolated, using a secondary site XcI857 lysogen in which we have shown the prophage to be closely linked to sul +. sul+ transducing particles were detected frequently, at 10-5 per plaque-forming unit, in lysates prepared from the secondary-site lysogens. High-frequency transducing lysates were obtained from several independently isolated sul + transductants and were analyzed by CsCl equilibrium density gradient centrifugation. The transducing phages are defective; marker rescue analysis indicates that the XN gene is not present. In XcI857ANdSu1+, a bio-type transducing phage, the genes specifying recombination and excision functions have been replaced by bacterial deoxyribonucleic acid. 120 on July 31, 2020 by guest http://jb.asm.org/ Downloaded from VOL. 122, 1975 sul+ TRANSDUCING BACTERIOPHAGES OF E. COLI 122 STEEGE AND LOW

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Non-random distribution of deletion endpoints in the gal operon of E. coli

Cited by 19 publications

References 18 publications

Deletion mapping of theniiAniaD gene region ofAspergillus nidulans

Deletion mapping of theniiAniaD gene region ofAspergillus nidulans

Deletion Analysis of Essential Genes of Escherichia coli: Investigation of the btuB-rpoBC Interval

Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli

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