2012
DOI: 10.1107/s0909049512044408
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Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images

Abstract: The typical dose used to record cryo-electron microscopy images from vitrified biological specimens is so high that radiation-induced structural alterations are bound to occur during data acquisition. Integration of all scattered electrons into one image can lead to significant blurring, particularly if the data are collected from an unsupported thin layer of ice suspended over the holes of a support film. Here, the dose has been fractioned and exposure series have been acquired in order to study beam-induced … Show more

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Cited by 5 publications
(2 citation statements)
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“…The generally preferred method is to add 5-20 nm gold nanoparticles to the sample prior to vitrification to act as fiducials during tilt-series alignment (Gruska et al, 2008;Dobro et al, 2017). The use of fiducials allows for accurate tilt-series alignment by correcting for non-rigid registration due to beam and stage-induced shifts as well as sample alterations (Karimi Nejadasl et al, 2013;Mastronarde and Held, 2017;Fernandez et al, 2018Fernandez et al, , 2019. However, adding fiducials to FIB-milled lamellae of eukaryotic cells has so far proven to be difficult.…”
Section: Introductionmentioning
confidence: 99%
“…The generally preferred method is to add 5-20 nm gold nanoparticles to the sample prior to vitrification to act as fiducials during tilt-series alignment (Gruska et al, 2008;Dobro et al, 2017). The use of fiducials allows for accurate tilt-series alignment by correcting for non-rigid registration due to beam and stage-induced shifts as well as sample alterations (Karimi Nejadasl et al, 2013;Mastronarde and Held, 2017;Fernandez et al, 2018Fernandez et al, , 2019. However, adding fiducials to FIB-milled lamellae of eukaryotic cells has so far proven to be difficult.…”
Section: Introductionmentioning
confidence: 99%
“…The generally preferred method is to add 5-20 nm gold nanoparticles to the sample prior to vitrification to act as fiducials during tilt-series alignment (Gruska et al, 2008;. The use of fiducials allows for accurate tilt-series alignment by correcting for non-rigid registration due to beam and stage-induced shifts as well as sample alterations (Karimi Nejadasl et al, 2013;. However, adding fiducials to FIB-milled lamellae of eukaryotic cells has so far proven to be difficult.…”
Section: Introductionmentioning
confidence: 99%