Non‐selective conductance in calcium channels of frog muscle: calcium selectivity in a single‐file pore.

Almers, Wolfhard; McCleskey, Edwin W.

doi:10.1113/jphysiol.1984.sp015352

Cited by 615 publications

(539 citation statements)

References 34 publications

Supporting

Mentioning

496

Contrasting

Order By: Relevance

“…Similar to other highly Ca 2ϩ -selective conductances, including both voltage-gated Ca 2ϩ channels and CRAC channels, complete removal of extracellular divalent cations reveals a significant permeability to monovalent cations through ARC channels (19). Based largely on extensive study of this phenomenon in voltage-gated Ca 2ϩ channels, this is believed to reflect the effects of a Ca 2ϩ -binding site within the channel pore (29,30). Occupation of this site by Ca 2ϩ precludes the permeation of monovalent ions, and such permeation only becomes possible when this site is vacant.…”

Section: Resultsmentioning

confidence: 99%

Ca2+ Selectivity and Fatty Acid Specificity of the Noncapacitative, Arachidonate-regulated Ca2+ (ARC) Channels

Mignen¹,

Thompson

Shuttleworth

2003

Journal of Biological Chemistry

100

103

View full text Add to dashboard Cite

The arachidonate-regulated, Ca 2؉ -selective ARC channels represent a novel receptor-activated pathway for the entry of Ca 2؉ in nonexcitable cells that is entirely separate from the widely studied store-operated, Ca 2؉ release-activated Ca 2؉ channels. Activation of ARC channels occurs specifically at the low agonist concentrations typically associated with oscillatory 2؉ selectivity of ARC channels is extremely high. Examination of the ability of various fatty acids, including arachidonic acid, to activate the ARC channels demonstrated that activation does not reflect any nonspecific membrane fluidity or detergent effects, shows a high degree of specificity for arachidonic acid over other fatty acids (especially monounsaturated and saturated fatty acids), and is independent of any arachidonic acid metabolite. Moreover, studies using the charged analogue arachidonyl coenzyme A demonstrate that activation of the ARC channels reflects an action of the fatty acid specifically at the internal face of the plasma membrane. Whether this involves a direct action of arachidonic acid on the channel protein itself or an action on some intermediary molecule is, at present, unclear.

show abstract

Section: Resultsmentioning

confidence: 99%

Ca2+ Selectivity and Fatty Acid Specificity of the Noncapacitative, Arachidonate-regulated Ca2+ (ARC) Channels

Mignen¹,

Thompson

Shuttleworth

2003

Journal of Biological Chemistry

100

103

View full text Add to dashboard Cite

show abstract

“…4B.). However, it has been often found that Ca 2ϩ channels possess multiple ion binding sites and show a high affinity binding of Ca 2ϩ but a low affinity for Ca 2ϩ conductance (45).…”

Section: Discussionmentioning

confidence: 99%

“…This hypothesis was directly tested by measuring Ca 2ϩ uptake rates. Uptake assays using 45 Ca 2ϩ demonstrated an LCT1-dependent increase in Ca 2ϩ uptake (Fig. 6).…”

Section: Lct1 Expression Leads To An Increase In CDmentioning

confidence: 99%

“…INVSc1 cells transformed with LCT1 in pYES2 (s, F) and with the empty pYES2 control plasmid (ᮀ, E) were grown in argininephosphate medium to an OD of Ϸ0.2 and assayed for calcium uptake with 45 Ca 2ϩ . Cells were incubated in uptake solution, containing 100 M Ca 2ϩ , at 30°C (F, E) or 0°C (s, ᮀ).…”

Section: Fig 6 Effect Of Lct1 Expression On Calcium Uptake In Yeastmentioning

confidence: 99%

See 1 more Smart Citation

The plant cDNA LCT1 mediates the uptake of calcium and cadmium in yeast

Clemens

Antosiewicz

Ward

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

255

141

View full text Add to dashboard Cite

show abstract

“…It may be that a better fit could be obtained using more complicated models such as the threebarrier two-well model which has been developed in heart cells by Hess & Tsien (1984; see also Almers & McCleskey, 1984) but we have not yet tested such models.…”

Section: Arsenazoiii In Cra Yfish Neuronesmentioning

confidence: 99%

Arsenazo III transients and calcium current in a normally non‐spiking neuronal soma of crayfish.

Bruner¹,

Czternasty²,

Shimahara³

et al. 1986

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Arsenazo III was used to investigate Ca2+ transients in the normally non-excitable soma of the motor giant neurones of the crayfish Procambaru8 clarkii.2. Two kinds of regenerative potentials could be obtained depending on membrane potential conditioning: a fast spike after a pre-hyperpolarization to -90 mV and a slow action potential after a pre-depolarization to -50 mV. Only the second of these was accompanied by an Arsenazo III transient.3. In voltage-clamped, somata injected, with tetraethylammonium chloride, an absorbance change could be obtained by pulsing the membrane potential above -44 mV. The relationship between absorbance change and potential peaked between 0 and + 10 mV then fell off to zero at ca. + 150 mV.4. Changes in light absorbance studied using double-pulse protocols suggested that the inactivation of Ca2+ entry was predominantly mediated by the intracellular free Ca2+ concentration.5. External application of 1 mM-CdCl2 abolished both the absorbance changes and the (Ca2+) inward current. The voltage dependence of this current was similar to that of the absorbance change.6. For positive membrane potential the current-voltage relationship showed a voltage-dependent conductance property, the origin of which is discussed.

show abstract

Non‐selective conductance in calcium channels of frog muscle: calcium selectivity in a single‐file pore.

Cited by 615 publications

References 34 publications

Ca2+ Selectivity and Fatty Acid Specificity of the Noncapacitative, Arachidonate-regulated Ca2+ (ARC) Channels

Ca2+ Selectivity and Fatty Acid Specificity of the Noncapacitative, Arachidonate-regulated Ca2+ (ARC) Channels

The plant cDNA LCT1 mediates the uptake of calcium and cadmium in yeast

Arsenazo III transients and calcium current in a normally non‐spiking neuronal soma of crayfish.

Contact Info

Product

Resources

About