Non-Selectivity of R-S Media for Aeromonas hydrophila and TCBS Media for Vibrio Species Isolated from Diseased Oreochromis niloticus

Aboyadak, Ibrahim Mohamed; Ali, Nadia Gm; Goda, Ashraf; Saad, Walaa; Salam, Asmaa Me

doi:10.4172/2155-9546.1000496

Cited by 16 publications

(12 citation statements)

References 16 publications

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“…It was performed as described by Aboyadak et al . [20], in which five samples were taken from each fish (tail lesions, muscle lesions, eye lesions if present, hepatopancreas, and heart blood). Each sample was individually inoculated to Tryptic Soy Broth for 12 h, then streaked on tryptic soy agar (Oxoid ® ), and incubated at 33°C for 18-24 h.…”

Section: Methodsmentioning

confidence: 99%

Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock

Ali

2019

Vet World

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Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease affecting white sea bream brooders and determining the sensitivity of the recovered isolates to different antibiotics followed by estimation of long-acting oxytetracycline (OTC) efficacy in controlling this disease, and finally, determining the proper dose regimen. Materials and Methods: Biolog microbial identification system was used for determination of the pathogens which are responsible for this disease. Agar disk diffusion test and minimum inhibitory concentration (MIC) were used to determine the antibiotic susceptibility of recovered isolates. Oxytetracycline (OTC) was used at a dose level of 100 mg/kg body weight for the treatment of diseased fish, and the OTC concentration in the serum samples was determined by high-performance liquid chromatography. Results: Fifteen Staphylococcus epidermidis and 11 Bacillus cereus isolates were recovered from the lesion of muscle, tail, eye, and heart blood. S. epidermidis isolates were sensitive to OTC, ciprofloxacin, enrofloxacin, spiramycin, erythromycin (E), and florfenicol. B. cereus isolates were sensitive to all mentioned antibiotics except E. Based on the MIC test, all B. cereus isolates were sensitive to OTC with MIC ranging between <0.125 and 4 μg/ml and 11 S. epidermidis isolates were sensitive with MIC ranging between <0.125 and 8 μg/ml, while four isolates were resistant. Different degrees of degenerative changes were present in the hepatopancreas, posterior kidney, eye, and skin tissues of diseased fish. Conclusion: Single intraperitoneal injection of long-acting OTC at a dose of 100 mg/kg body weight was effective in termination of S. epidermidis and B. cereus infection in white sea bream (D. sargus) broodstock.

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Section: Methodsmentioning

confidence: 99%

Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock

Ali

2019

Vet World

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“…A. hydrophila, one of the most vigorous Aeromonas species, able to induce incurable problems to freshwater fish aquaculture in Egypt (Aboyadak et al, 2017) and has zoonotic importance with the capability to possess multiple antimicrobial resistance toward different classes of antibiotics (Le et al, 2018) . Multifactor and the virulence genes work together to induce the virulence of A. hydrophila which contribute to infect, spread and kill the host such as cytotoxic enterotoxin gene, aerolysin gene, cytotonic enterotoxin heat-labile gene, cytotonic enterotoxin heat-stable gene, lipases, proteases, beta-lactamases, amylases, chitinases and nucleases with disease development (Furmanek-Blaszk, 2014;Stratev et al, 2015) .…”

Section: Introductionmentioning

confidence: 99%

Virulent and Multiple Antimicrobial Resistance <i>Aeromonas hydrophila</i> Isolated from Diseased Nile Tilapia Fish (<i>Oreochromis niloticus</i>) in Egypt with Sequencing of Some Virulence-Associated Genes

Saleh¹,

Elkenany

Younis

2021

Biocontrol Sci.

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Aeromonas hydrophila is a major waterborne pathogen, which induces various diseases in freshwater fish with the capability for zoonotic potential. This study was applied to investigate the prevalence of A. hydrophila in diseased Nile tilapia fish, genetic characterization of the virulence encoding genes (act, aerA, alt, and ast genes) , and antibiotic susceptibility. Out of the 500 diseased Nile tilapia fish samples, 70% (350/500) Aeromonas species were isolated. From which 53.4% (187/350) of Aeromonas hydrophila strains were identified. A. hydrophila was detected in kidneys, followed by liver, spleen, intestine, and gills. The results of virulotyping displayed the presence of act, and aerA genes in a high percentage of 40%, followed by alt gene (30%) , but ast gene was not detected (0%) in A. hydrophila strains. Based on DNA sequence analysis of three virulence associated-genes (act, aerA, and alt genes) , the phylogenetic tree showed the genetic relationship with related species. Finally, the antibiotic susceptibility tests revealed high resistance toward chloramphenicol (67.4%) , followed by amikacin (51.9%) and gentamicin (47.1%) , whereas a high sensitivity was exhibited toward meropenem (90.9%) , followed by ciprofloxacin (84.2%) , amoxicillin-clavulanic acid (73.3%) and trimethoprimsulfamethoxazole (64.2%) . The multidrug-resistant A. hydrophila strains were observed in 69.0% of strains with six resistance patterns.

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“…Aeromonas spp. can grow on Vibrio isolation agars like TCBS (thiosulfate-citrate-bile-salt-sucrose agar) and thus be misidentified as being a Vibrio [58]. In the following, the taxonomy, virulence and prevalence of the bacteria belonging to genus Aeromonas are discussed.…”

Section: The Rte Seafood Trend From a Food Safety Perspectivementioning

confidence: 99%

The Significance of Mesophilic Aeromonas spp. in Minimally Processed Ready-to-Eat Seafood

2019

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Minimally processed and ready-to-eat (RTE) seafood products are gaining popularity because of their availability in retail stores and the consumers’ perception of convenience. Products that are subjected to mild processing and products that do not require additional heating prior to consumption are eaten by an increasing proportion of the population, including people that are more susceptible to foodborne disease. Worldwide, seafood is an important source of foodborne outbreaks, but the exact burden is not known. The increased interest in seafood products for raw consumption introduces new food safety issues that must be addressed by all actors in the food chain. Bacteria belonging to genus Aeromonas are ubiquitous in marine environments, and Aeromonas spp. has held the title “emerging foodborne pathogen” for more than a decade. Given its high prevalence in seafood and in vegetables included in many RTE seafood meals, the significance of Aeromonas as a potential foodborne pathogen and a food spoilage organism increases. Some Aeromonas spp. can grow relatively uninhibited in food during refrigeration under a broad range of pH and NaCl concentrations, and in various packaging atmospheres. Strains of several Aeromonas species have shown spoilage potential by the production of spoilage associated metabolites in various seafood products, but the knowledge on spoilage in cold water fish species is scarce. The question about the significance of Aeromonas spp. in RTE seafood products is challenged by the limited knowledge on how to identify the truly virulent strains. The limited information on clinically relevant strains is partly due to few registered outbreaks, and to the disputed role as a true foodborne pathogen. However, it is likely that illness caused by Aeromonas might go on undetected due to unreported cases and a lack of adequate identification schemes. A rather confusing taxonomy and inadequate biochemical tests for species identification has led to a biased focus towards some Aeromonas species. Over the last ten years, several housekeeping genes has replaced the 16S rRNA gene as suitable genetic markers for phylogenetic analysis. The result is a more clear and robust taxonomy and updated knowledge on the currently circulating environmental strains. Nevertheless, more knowledge on which factors that contribute to virulence and how to control the potential pathogenic strains of Aeromonas in perishable RTE seafood products are needed.

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Non-Selectivity of R-S Media for Aeromonas hydrophila and TCBS Media for Vibrio Species Isolated from Diseased Oreochromis niloticus

Cited by 16 publications

References 16 publications

Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock

Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock

Virulent and Multiple Antimicrobial Resistance <i>Aeromonas hydrophila</i> Isolated from Diseased Nile Tilapia Fish (<i>Oreochromis niloticus</i>) in Egypt with Sequencing of Some Virulence-Associated Genes

The Significance of Mesophilic Aeromonas spp. in Minimally Processed Ready-to-Eat Seafood

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