This study investigates the various uses of density of motile microorganisms in the context of the flow of a binary base fluid with double diffusion past a vertical surface. The research aims to comprehend the interactions between motile microorganisms and the fluid dynamics, as well as the heat and mass transport mechanisms in this system. The analysis involves mathematically constructing the governing equations, transforming them into dimensionless nonlinear ordinary differential equations using similarity transformations, and numerically solving them using the MATLAB bvp4c solver. An analysis of the influence of several parameters on the profiles of velocity, temperature, concentration, nanoparticle concentration, and density of motile microorganisms is conducted using graphical representation. The findings demonstrate that boosting the thermophoresis parameter intensifies the temperature profile. In addition, an increase in the nanofluid Schmidt number results in a larger concentration of nanoparticles, whereas a higher bioconvection Lewis number reduces the density of the motile microorganism profile. These findings may find use in biomedical engineering as well as industrial processes that include enhancing the efficiency of mass transfer and bioconvection. Numeric simulation prophesies 99.9% for both shear stress and heat transfer rate intensification for Prandtl values are noticed.