Non-Specific Adsorption of Streptavidin on Single Walled Carbon Nanotubes

González, Mónica C.; Tort, Nuria; Benito, Ana M.; Maser, W.K.; Marco, M.‐Pilar; Martínez, María Teresa

doi:10.1166/jnn.2009.1576

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…This was a consequence of the full coverage of the CNTs in the devices in streptavidin (see Figure 4) which prevented other biomolecules from being able to be absorbed on the CNT surface. As previously reported by the authors, 33 covalent and noncovalent modification of SWCNTs with polymers, surfactants, and functional groups is not capable of preventing the absorption of streptavidin. Likewise, the anchoring of a polymer was capable of preventing the aggregation of DNA in the electrodes changing the Schotky barrier, 52 but it was not successful in preventing the absorption of streptavidin.…”

Section: ■ Results and Discussionsupporting

confidence: 58%

“…They found that the modified SWNTs were resistant to proteins such as streptavidin, avidin, bovine serum albumin, glucosidase, and staphylococcal protein A. The authors 33 tested different strategies, trying unsuccessfully to prevent nonspecific adsorption of streptavidin on SWNTs. The hydrophobic character of the SWNTs was modified by surface functionalization with oxygenated groups, amine groups, and PEG, which rendered the SWNTs surface more hydrophilic.…”

Section: ■ Introductionmentioning

confidence: 99%

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Streptavidin as CNTs and DNA Linker for the Specific Electronic and Optical Detection of DNA Hybridization

et al. 2012

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Streptavidin was used as an interlinker between single-wall carbon nanotubes (SWNTs) and oligonucleotides for the electronic and optical detection of DNA hybridization. The aim of using streptavidin as a linker was to prevent nonspecific adsorption of biomolecules onto carbon nanotubes (CNTs) and also to prevent the aggregation of DNA strands or other contaminants to the source and drain electrodes that can modify the Schottky barrier. The electronic detection was performed by using arrays of CNTs field effect transistors (CNTFETs) and statistically significant changes in the threshold voltage and ION current were inferred. For the optical approach, a complementary DNA strand labeled with a dye was used, and the hybridization was detected by fluorescence microscopy. Streptavidin was noncovalently bonded to SWCNTs. Afterward, single-strand DNA modified with biotin was attached to the streptavidin, followed by DNA hybridization with the complementary DNA strand. Direct addition of the complementary DNA strand to the SWNTs covered with streptavidin was also performed to rule out any false hybridization positives due to nonspecific adsorption, and neither electrical nor optical responses were recorded. The results indicated that streptavidin efficiently prevents nonspecific adsorption of high-affinity molecules such as DNA onto CNTs. The wrapping of SWNTs with streptavidin allows both electrical and fluorescence detection of DNA hybridization.

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Section: ■ Results and Discussionsupporting

confidence: 58%

Section: ■ Introductionmentioning

confidence: 99%

Streptavidin as CNTs and DNA Linker for the Specific Electronic and Optical Detection of DNA Hybridization

et al. 2012

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“…The objective of using the polymer was to avoid an aggregation of antibodies on the electrodes that could modify the metal work function and the Schottky barrier and also to prevent the nonspecific adsorption of proteins and biomolecules on the CNTFET. Strong binding between the nanotubes and proteins has been reported by several authors. ,− This is an important issue when the detection is carried out in a serum environment.…”

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confidence: 95%

Electronic Anabolic Steroid Recognition with Carbon Nanotube Field-Effect Transistors

et al. 2010

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A proof of concept of the electronic detection of two anabolic steroids, stanozolol (Stz) and methylboldenone (MB), was carried out using two specific antibodies and arrays of carbon nanotube field-effect transistors (CNTFETs). Antibodies specific for Stz and MB were prepared and immobilized on the carbon nanotubes (CNTs) using two different approaches: direct noncovalent bonding of antibodies to the devices and bonding the antibodies covalently to a polymer previously attached to the CNTFETs. The results indicated that CNTFETs bonded to specific antibodies covalently or noncovalently are able to detect the presence of steroids. Statistically significant changes in the threshold voltage and drain current were registered in the transistors, allowing the steroids to be recognized. On the other hand, it was determined that the specific antibodies do not detect other steroids other than Stz and MB, such as nandrolone (ND) because, in this case, statistically significant changes in the transistors were not detected. The polymer prevents the aggregation of antibodies on the electrodes and decreases the transistor hysteresis. Nevertheless, it is not able to avoid the nonspecific adsorption of streptavidin, meaning that nonspecific adsorption on CNTs remains a problem and that this methodology is only useful for purified samples. Regarding the detection mechanism, in addition to charge transfer, Schottky barrier, SB, modification, and scattering potential reported by other authors, an electron/hole trapping mechanism leading to hysteresis modification has been determined. The presence of polymer seems to hinder the modulation of the electrode-CNT contact.

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