Non-Specific Adsorption Reduction Methods in Biosensing

Lichtenberg, Jessanne Y.; Ling, Yue; Kim, Seunghyun

doi:10.3390/s19112488

Cited by 191 publications

(144 citation statements)

References 100 publications

(209 reference statements)

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“…However, conventional antibody-based biosensors cannot distinguish non-specific adsorption-derived signals from targetspecific ones due to their mere binding-based mechanisms. 70,83 Apart from the perspective of current limits, how do cells and our bodies accurately discriminate similar molecules? During complex yet precise cellular signaling processes and enzymatic reactions, relevant membrane receptors and enzyme proteins increase their target selectivity by using binding-induced structural change and stabilization of transition state.…”

Section: Increasing Selectivitymentioning

confidence: 99%

Detection and beyond: challenges and advances in aptamer-based biosensors

Yoo

2020

Mater. Adv.

189

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Section: Increasing Selectivitymentioning

confidence: 99%

Detection and beyond: challenges and advances in aptamer-based biosensors

Yoo

2020

Mater. Adv.

189

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“…This is due to the relatively low concentrations of biomarkers within water environments [133]. Additionally, in complex biological samples, sensitivity of biosensors may be decreased due to low levels of non-specific binding [134,135]. There are several different methods for the preconcentration of proteins and cells as well as the amplification of nucleic acid biomarkers used regularly within laboratory settings.…”

Section: How To Detect On-sitementioning

confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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More than 783 million people worldwide are currently without access to clean and safe water. Approximately 1 in 5 cases of mortality due to waterborne diseases involve children, and over 1.5 million cases of waterborne disease occur every year. In the developing world, this makes waterborne diseases the second highest cause of mortality. Such cases of waterborne disease are thought to be caused by poor sanitation, water infrastructure, public knowledge, and lack of suitable water monitoring systems. Conventional laboratory-based techniques are inadequate for effective on-site water quality monitoring purposes. This is due to their need for excessive equipment, operational complexity, lack of affordability, and long sample collection to data analysis times. In this review, we discuss the conventional techniques used in modern-day water quality testing. We discuss the future challenges of water quality testing in the developing world and how conventional techniques fall short of these challenges. Finally, we discuss the development of electrochemical biosensors and current research on the integration of these devices with microfluidic components to develop truly integrated, portable, simple to use and cost-effective devices for use by local environmental agencies, NGOs, and local communities in low-resource settings.

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“…Nonspecific adsorption has been one of the main roadblocks against utilizing electrochemical sensors in real-life applications because it tends to significantly decrease sensitivity, specificity, and reproducibility of the sensors. In the point-of-care, researchers are tackling this challenge with innovative materials and methods to improve sensor performance (Li Y. et al, 2018;Lichtenberg et al, 2019). Both passive and active methods have been used.…”

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confidence: 99%

“…Passive methods aim to create a hydrophilic and non-charged layer to obstruct protein adsorption on the surface by using different organic materials such as polymers. Conversely, the aim of active methods is to produce surface shear forces that are stronger than the adhesion forces of the bound non-specific biomolecules on the surface (Li Y. et al, 2018;Lichtenberg et al, 2019).…”

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confidence: 99%

Grand Challenges in Nanomaterial-Based Electrochemical Sensors

Ferrag

Kerman

2020

Front. Sens.

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Electrochemical sensors have an enormous potential in a wide variety of environmental, industrial, and medicinal applications. Apart from the immense success of glucose sensors, much more work is still needed in order to make electrochemical sensors have a widespread impact and application. For example, the current circumstances of the COVID-19 pandemic demonstrated the importance and urgency of having accurate and rapid diagnostic devices (Jiang et al., 2020). The advancement of sensors could truly help stop the spread of many infectious diseases (Vermisoglou et al., 2020) and detect the early onset of various illnesses such as neurodegenerative diseases (Kim et al., 2020). Compared to other diagnostic tools currently available, electrochemical sensors have many advantages such as low-cost, rapid and real-time detection with simple operation (Idili et al., 2019; Ligler and Gooding, 2019). They can also be mass-produced and miniaturized into portable devices (Li et al., 2017; Idili et al., 2019; Ligler and Gooding, 2019). The number of research groups reporting the development of novel electrochemical sensors is growing exponentially. Most of the reported sensors have carbon-and gold-based surfaces. These surfaces are popular amongst researchers because they are stable, biocompatible, and provide good electron transfer kinetics. Unfortunately, the unmodified surfaces often lack the sensitivity and selectivity required for the electrochemical detection of trace analytes. To overcome this challenge, nanomaterials have been incorporated within the electrode surfaces (Quesada-González and Merkoçi, 2018; Muniandy et al., 2019). Nanomaterials range from 1-100 nm in size and are extremely beneficial due to the large surface-to-volume ratio and surface area (Quesada-González and Merkoçi, 2018; Muniandy et al., 2019). Modification of nanomaterials on sensor surfaces allows them to have enhanced interfacial adsorption with improved electrocatalytic activity, biocompatibility, and faster electron transfer kinetics. All these advantages give the sensor a better selectivity and sensitivity toward the detection of specific analytes as well as a superior overall performance (

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Non-Specific Adsorption Reduction Methods in Biosensing

Cited by 191 publications

References 100 publications

Detection and beyond: challenges and advances in aptamer-based biosensors

Detection and beyond: challenges and advances in aptamer-based biosensors

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

Grand Challenges in Nanomaterial-Based Electrochemical Sensors

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