Non-specific immune responses in juveniles of Indian major carps

Abstract

The non-specific immune parameters are useful to determine the health status of fish and to evaluate the immunomodulatory substances for fish farming as markers of pollution and disease resistance. Some of the important parameters, viz. superoxide production by neutrophils through nitroblue tetrazolium (NBT) assay; haemagglutination (HA), haemolysin (HLY) and bacterial agglutination titres; myeloperoxidase (MPO), and lysozyme activities, and alternative complement levels in serum of the juveniles of three Indian major carp species (Cirrhinus mrigala, Catla catla and Labeo rohita) were measured to establish their physiological normal range. A wide variation among the individuals within a species in the ranges of the most of the immune parameters was recorded. Significantly higher levels in the mean values of HA, HLY, bacterial agglutination titres; superoxide production by neutrophils in nitroblue tetrazolium assay; serum MPO and lysozyme activities, i.e. 371.20, 4.60, 18.80, 0.40, 0.62 and 6.55 lg ml )1 , respectively, were obtained in L. rohita except a much lower alternative haemolytic complement activity (29.06 units ml )1 ) compared with the other two species. In most of the parameters, L. rohita showed the highest value, possibly indicating its more natural resistance compared with the other two species.

Effect of Culture Conditions on the Levels of Serum Insulin-like Growth Factor-1 in Indian Major Carps

“…The observed significant differences in the IGF-1 levels of bottom dwelling C. mrigala of normal farm and sewage-fed farm, as well as captive reared and sewage-fed farm indicated that the growth of C. mrigala is adversely affected by sewage, possibly due to the long-term exposure, and prevailing water quality and unfavourable pond bottom conditions. The results further corroborate the observations of Sahoo et al (2005), who recorded better health status of L. rohita and C. catla than C. mrigala in aquaculture conditions on the basis of respiratory activity. Hayat et al (2007) reported significantly lower weight increments in C. mrigala among IMCs exposed to sub-lethal concentrations of heavy metals, viz., Fe, Zn, Pb, Ni, Mn and their mixture for 90 days culture.…”

“…Fish growth rates vary with season, age and nutritional status. A number of factors such as condition factor (Carlander, 1969), organosomatic indices (Goede and Barton, 1990), superoxides (Sahoo et al, 2005), insulin-like growth factor (IGF)-1, and IGF binding proteins (Picha et al, 2012) have been proposed to assess the growth potential and health of culturable species. Most of these regulatory events are mediated by the growth hormone (GH) and IGF axis (Pé rez-Sá nchez et al, 2002;Shimizu et al, 2006).…”

Effects of Curcumin Supplemented Diet on Growth and Non-Specific Immune Parameters of Cirrhinus mrigala against Edwardsiella tarda Infection

“…NBT activity is typically associated with respiratory burst activity, characterized by intracellular localization of super oxide anion in phagocytes (Sahoo et al, 2005). Phagocytes after activation generate super oxides and other reactive compounds (i.e.…”

HEMAGGLUTINATION ANTIBODY TITERS IN PACU, Piaractus mesopotamicus, AS AN INDICATOR OF ACQUIRED IMMUNITY/Título de anticorpos hemaglutinantes de Pacu, Piaractus mesopotamicus, como indicador de imunidade adquirida

“…The hemagglutination is a reaction with particulate antigens and such reaction occurs only with antigens located on the cell surface and often the identification of these antigens can occur naturally without the need for prior immunization, as verified by , Sahoo et al (2005) and Dash et al (1993). Antibodies that perform the hemagglutination are usually multivalent whilst the isotypic class -IgM has higher binding activity.…”