Non‐Steroidal Anti‐Inflammatory Agents, Part 20 ^[1] Method for Testing Non‐Steroidal Anti‐Inflammatories: The Modified Hen's Egg Chorioallantoic Membrane Test (HET‐CAM Test) Compared to Other Procedures

Abstract

The delay of onset of irritation phenomena at the chorioallantoic membrane of incubated hen's eggs, a parameter for anti-inflammatory activity, was determined for the pharmaceutical substances diclofenac, flufenamic acid, ibuprofen, indomethacin, ketoprofen, piroxicam, phenylbutazone, salicylic acid, and sodium salicylate. Alongside questions relating to the dose-effect ratio, metabolisation, recovery, and diffusion of the substances to their site of action were investigated. The reproducibility of the procedure and its selectivity with regard to substances with a different mechanism of action is proven. The method allows classification of the substances according to their anti-inflammatory potency. However, correlation with the results of enzyme inhibition or in vivo results is only possible to a limited extent.

Gewebetoxizität lokaler Anästhetika im HET-CAM-Test

“…Der HET-CAM-Test ist ein u. a. in der pharmazeutischen, ophthalmologischen und zahnmedizinischen Forschung etabliertes Replacementverfahren für tierexperimentelle Studien an höherentwickel-ten Wirbeltieren, welches die In-vivoToxizitätskontrolle von Flüssigkeiten und Feststoffen an einem komplexen physiologischen System erlaubt [7,15,25,27,29,32]. Das Standardprotokoll ist in vielen EU-Staaten (u. a. Deutschland) zur Verträglichkeitstestung von Kosmetika [14] zugelassen.…”

In-vitro test system for the evaluation of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) inhibitors based on a single HPLC run with UV detection using bovine aortic coronary endothelial cells (BAECs)

“…Our previously published in vitro cell assays for the determination of COX-1/5-LOX and COX-1/thromboxane synthase (TXS) used bovine platelets, neutrophils and pig platelets as the enzyme source [9,10]. Moreover we used an alternative membrane assay (hen's egg chorioallantoic membrane) as a model for inflammation [11]. These assays show good reproducibility and comparability with human data.…”