Inverse spinel oxide NiCo 2 O 4 is an excellent prototype to explore complex energy landscapes among exchange interactions, crystal field effects, and chemical/lattice disorders. In this work, periodic phase-separated nanocolumn NiCo 2 O 4 films have been deposited on MgAl 2 O 4 substrates via pulsed laser deposition. The nanocolumn NiCo 2 O 4 is identified as an ideal inverse spinel NiCo 2 O 4 structure and an imperfect inverse spinel structure with Co vacancies at tetrahedral sites. Compared to the usual single-phase NiCo 2 O 4 film, a 20-fold enhancement of magnetoresistance (MRR = 6% at 10 K and 3 T) is observed in the semiconducting NiCo 2 O 4 film with columnlike phase separation, accompanying a 25-fold increase in coercivity (H C = 18.1 kOe at 10 K) due to the strong interfacial interaction at the vertical phase boundaries. The nanocolumn NiCo 2 O 4 also exhibits strong perpendicular magnetic anisotropy as well as a robust anomalous Hall effect (AHE) below the Curie temperature. In addition, a Hall resistance jump near the coercive field is observed, which is related to the domain rotation in the vicinity of the switching field. This study provides a phase-separated nanocolumn material with strong vertical coupling for designing artificial nanostructured spintronic devices.