Non-targeted metabolomic profiling of filamentous cyanobacteria Aphanizomenon flos-aquae exposed to a concentrated culture filtrate of Microcystis aeruginosa

Hu, Jin; Ma, Haiyan; Gan, Nanqin; Wang, Hongxia; Li, Yanhua; Wang, Lan; Song, Lirong

doi:10.1016/j.hal.2021.102170

Cited by 11 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it was found that the growth of Aphanizomenon is inhibited due to the allelopathic effect produced by Microcystis [ 27 ]. Recently, a study found that the growth inhibition of Aphanizomenon by Microcystis is unlikely to result from a single metabolite [ 28 ]. Instead, multiple metabolites, such as sphingolipids, glycerolipids, and succinylacetone, are involved in inducing the adverse phenotype.…”

Section: Discussionmentioning

confidence: 99%

Spatial and Temporal Variability of Saxitoxin-Producing Cyanobacteria in U.S. Urban Lakes

Jeon,

Struewing,

McIntosh

et al. 2024

Toxins

View full text Add to dashboard Cite

Harmful cyanobacterial blooms (HCBs) are of growing global concern due to their production of toxic compounds, which threaten ecosystems and human health. Saxitoxins (STXs), commonly known as paralytic shellfish poison, are a neurotoxic alkaloid produced by some cyanobacteria. Although many field studies indicate a widespread distribution of STX, it is understudied relative to other cyanotoxins such as microcystins (MCs). In this study, we assessed eleven U.S. urban lakes using qPCR, sxtA gene-targeting sequencing, and 16S rRNA gene sequencing to understand the spatio-temporal variations in cyanobacteria and their potential role in STX production. During the blooms, qPCR analysis confirmed the presence of the STX-encoding gene sxtA at all lakes. In particular, the abundance of the sxtA gene had a strong positive correlation with STX concentrations in Big 11 Lake in Kansas City, which was also the site with the highest quantified STX concentration. Sequencing analysis revealed that potential STX producers, such as Aphanizomenon, Dolichospermum, and Raphidiopsis, were present. Further analysis targeting amplicons of the sxtA gene identified that Aphanizomenon and/or Dolichospermum are the primary STX producer, showing a significant correlation with sxtA gene abundances and STX concentrations. In addition, Aphanizomenon was associated with environmental factors, such as conductivity, sulfate, and orthophosphate, whereas Dolichospermum was correlated with temperature and pH. Overall, the results herein enhance our understanding of the STX-producing cyanobacteria and aid in developing strategies to control HCBs.

show abstract

Section: Discussionmentioning

confidence: 99%

Spatial and Temporal Variability of Saxitoxin-Producing Cyanobacteria in U.S. Urban Lakes

Jeon,

Struewing,

McIntosh

et al. 2024

Toxins

View full text Add to dashboard Cite

show abstract

“…Untargeted comparative metabolite profiling was preferred over targeted metabolomics to identify multiple pathways in the targeted organism (this study- A. torulosa ) that are affected by inoculation and coculturing with fungal - Tr , and bacterial -PW5 partners, as against other abiotic/biotic stresses reported in earlier studies ( Jin et al., 2022 ). Varous studies have been undertaken to understand the influence of microbial metabolites in improving plant growth.…”

Section: Discussionmentioning

confidence: 99%

Untargeted GC–MS reveals differential regulation of metabolic pathways in cyanobacterium Anabaena and its biofilms with Trichoderma viride and Providencia sp.

Nishanth

Prasanna

2022

Current Research in Microbial Sciences

View full text Add to dashboard Cite

“…They are known as nitrogen fixers because they can convert atmospheric nitrogen into a form that plants can utilize. As a result, they contribute nutrients to the food chain and maintain high water quality, making them vital to aquatic environments [ 35 , 36 ]. Certain Aphanizomenon species can harm humans and other animals when present in more significant proportions than usual.…”

Section: Common Genera Of Cyanobacteria and Their Characteristicsmentioning

confidence: 99%

Understanding the Risks of Diffusion of Cyanobacteria Toxins in Rivers, Lakes, and Potable Water

Thawabteh,

Naseef,

Karaman

et al. 2023

Toxins

View full text Add to dashboard Cite

Blue-green algae, or cyanobacteria, may be prevalent in our rivers and tap water. These minuscule bacteria can grow swiftly and form blooms in warm, nutrient-rich water. Toxins produced by cyanobacteria can pollute rivers and streams and harm the liver and nervous system in humans. This review highlights the properties of 25 toxin types produced by 12 different cyanobacteria genera. The review also covered strategies for reducing and controlling cyanobacteria issues. These include using physical or chemical treatments, cutting back on fertilizer input, algal lawn scrubbers, and antagonistic microorganisms for biocontrol. Micro-, nano- and ultrafiltration techniques could be used for the removal of internal and extracellular cyanotoxins, in addition to powdered or granular activated carbon, ozonation, sedimentation, ultraviolet radiation, potassium permanganate, free chlorine, and pre-treatment oxidation techniques. The efficiency of treatment techniques for removing intracellular and extracellular cyanotoxins is also demonstrated. These approaches aim to lessen the risks of cyanobacterial blooms and associated toxins. Effective management of cyanobacteria in water systems depends on early detection and quick action. Cyanobacteria cells and their toxins can be detected using microscopy, molecular methods, chromatography, and spectroscopy. Understanding the causes of blooms and the many ways for their detection and elimination will help the management of this crucial environmental issue.

show abstract

Non-targeted metabolomic profiling of filamentous cyanobacteria Aphanizomenon flos-aquae exposed to a concentrated culture filtrate of Microcystis aeruginosa

Cited by 11 publications

References 47 publications

Spatial and Temporal Variability of Saxitoxin-Producing Cyanobacteria in U.S. Urban Lakes

Spatial and Temporal Variability of Saxitoxin-Producing Cyanobacteria in U.S. Urban Lakes

Untargeted GC–MS reveals differential regulation of metabolic pathways in cyanobacterium Anabaena and its biofilms with Trichoderma viride and Providencia sp.

Understanding the Risks of Diffusion of Cyanobacteria Toxins in Rivers, Lakes, and Potable Water

Contact Info

Product

Resources

About