Non-Targeted Metabolomics Reveals Patterns of Metabolic Changes during Poplar Seed Germination

Qu, Chunpu; Chen, Jinyuan; Cao, Lina; Teng, Xiangjin; Li, Jinbo; Yang, Chengjun; Zhang, Xiuli; Zhang, Yuhong; Liu, Guanjun; Zhang, Xu

doi:10.3390/f10080659

Cited by 5 publications

(10 citation statements)

References 40 publications

(50 reference statements)

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“…Moreover, TCA-cycle was also significantly down-regulated relative to RPPDS. Non-Targeted metabolomics analysis of Poplar seeds also revealed that three TCA cycle intermediates (citrate, isocitrate and succinate) substantially accumulated during the periods of slow water uptake and then promptly decreased until radicle protrusion (Qu et al, 2019). Research conducted with non-dormant and dormant seeds of Leymus chinensis demonstrated that the proteins involved in TCA cycle were decreased in non-dormant seeds (Hou et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Key metabolic pathways involved in seed primary physiological dormancy maintenance of Korean pine seed

Song

Gao

2021

Preprint

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The metabolic changes that occurred during either cold stratification or after-ripen treatment, and in both dormant seeds and after-ripened seeds either under the dry state or during imbibition have been extensively explored. Much less is known about those present in both dormant seeds and cold stratified seeds during the same period of incubation under favorable germination conditions. Metabolite composition was investigated in both embryo and megagametophyte of primary physiological dormant seeds (PPDS) of Pinus Koreansis collected at 0 week, 1 week, 2 weeks, 4 weeks and 6 weeks of incubation, and of cold stratified seeds with released primary physiological dormancy (RPPDS) sampled at 0 week and 1 week of incubation, seed coat rupture stage and radicle protrusion stage. Embryo contained higher levels of most metabolites compared to megagametophyte. Strong metabolic changes occurred at 1 week and 4 weeks of incubation in PPDS, with most metabolites were significantly accumulated in 4-weeks-incubated PPDS. A larger metabolic switch was found in RPPDS between 1-week-incubation and seed coat rupture stage. Especially, there was a significant major decrease in the relative levels of most phosphorylated sugars and amino acids. The carbohydrate metabolism, especially pentose phosphate pathway and tricarboxylic acid cycle were more active pathways in the embryos of 4-weeks-incubated PPDS, but the operation rate of most amino acid metabolism was lower compared to 1-week-incubated RPPDS. We suggest that a larger metabolic switch in the embryo of PPDS after 4 weeks of incubation may assist in maintaining primary dormancy.

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Section: Discussionmentioning

confidence: 99%

Key metabolic pathways involved in seed primary physiological dormancy maintenance of Korean pine seed

Song

Gao

2021

Preprint

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“…The changes of seed storage substances during seed germination and pre-sprouting have been discussed in some cereal and legume crops and model woody plant, such as rice, barley, white, mung bean and poplar [6,21,24,32]. The above reports demonstrated that active metabolism processes occurred to provide nutrients and energy reserves for seed germination, but varying metabolite-metabolite links may exist in plant species [33,34].…”

Section: Discussionmentioning

confidence: 99%

“…Molecular studies on the imbibed-barley seeds have also shown that genes involved in amino acid metabolism are triggered after imbibitions36. In poplar, the involvement of amino acid metabolism is exclusive between the late stages of embryo water uptake to the completion of seedling growth [21]. To better understand the underlining mechanism during in-situ germination of nut crops, we explored the synergetic roles of metabolites in the nut kernels for regulation of in-situ germination of Carya species nut kernels using a comparative metabolomics approach in this study.…”

Section: Discussionmentioning

confidence: 99%

“…However, most of the studies on (in-situ) seed germination mechanisms focused on physiological, molecular and genetic aspects of several major crops or model plants [4][5][14][15][16]. In recent years, metabolomic approaches have been widely applied to explore the molecular mechanisms of seed life activities [17][18][19][20][21]. Metabolite quantitative trait loci (QTL) mapping and correlation-based metabolic network analysis connecting the morphological data revealed the central role of amino acids in coordinating interactions of other metabolites (sugars and organic acids) during tomato seed germination [17].…”

Section: Introductionmentioning

confidence: 99%

“…palmitate and linoleate), succinate and fumarate of thetricarboxylic acid (TCA) cycle and glycerol-3-phosphate during tomato seed germination [19]. Non-targeted metabolome study on germinating poplar seeds also revealed the crucial contribution of primary metabolite contents involving the galactose metabolism and alanine, aspartate, and glutamate metabolism [21]. Alteration on the regulations of lipids, fatty acids, amino acids, oxalate, and the raffinose family of oligosaccharides are found connected to the different germination status of dormant and susceptible wheat varieties [6].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Metabolome Analyses of Chinese Hickory Nut Kernels Highlight The Importance of Allocation Shift and Accumulation on Primary and Secondary Metabolites During In-Situ Germination

Xiao¹,

ong²,

Wang³

et al. 2020

Preprint

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Background: Chinese hickory ( Carya cathayensis ) and pecan ( C. illinoinensis ) are popular nut trees with high economic value in the family Juglandaceae, due to the richness in nutrients and multiple health factors in their nut kernels. However, the considerable in-situ germination of the nut seeds severely impact the quality of the kernels. The fundamental meta bolic mechanism of in-situ germination has been widely reported in major crops but rare in nut crops. Results: We sampled non- and pre-sprouted Chinese hickory nut kernels and performed liquid chromatography-tandem mass spectrometry (LC–MS/MS)-based metabolomic analyses. Comparative metabolomic analyses revealed 35 6 significantly-changed metabolites during in-situ germination. Detailed analysis emphasized the increasing accumulation and dynamic changes in compounds of primary mechanism and energy supply for in-situ germination. The results highlighted the importance of allocation shift and accumulation on four classes of primary metabolites and their derivatives, and two secondary metabolite classes between the transitions of germination initiation and germination and seedling establishment during the process. Metabolites related to carbohydrates and nitrogen metabolism, and few harmful to human health also demonstrated increasing accumulation along the process. Conclusions: The findings flourish our understanding of the metabolic mechanisms of in-situ germination and will be useful for future application in molecular breeding in Chinese hickory.

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Analysis of the energy source at the early stage of poplar seed germination: verification of Perl’s pathway

Zhang

Zhao

et al. 2020

3 Biotech

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Adenosine triphosphate (ATP) is produced at the early stage of seed germination and provides the energy for metabolism. The source of ATP in seeds may be Perl's pathway, but this has not yet been confirmed. In this study, using germinating seeds of poplar as the experimental materials, the transcript levels of genes related to Perl's pathway were determined by real-time PCR. The activities of enzymes in Perl's pathway were also determined. The results were verified by comparison with RNA-Seq and metabolomics data. The results showed that there were high transcript levels of some genes encoding malate dehydrogenase (MDH), phosphoenolpyruvate carboxykinase (PEPCK), pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH), and pyruvate kinase (PK) at the early stage of germination (0.75 h). The enzymes MDH, PEPCK, PK, PDC, and ADH showed peaks in activity at around 0.75 h and 6 h during germination. The oxaloacetate concentration was high in poplar seeds at the early stage of germination. This study provides experimental data showing that Perl's pathway participates in supplying energy during the early stages of poplar seed germination, and lays the foundation for further studies on the complex metabolic processes that function during seed germination.

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Non-Targeted Metabolomics Reveals Patterns of Metabolic Changes during Poplar Seed Germination

Cited by 5 publications

References 40 publications

Key metabolic pathways involved in seed primary physiological dormancy maintenance of Korean pine seed

Key metabolic pathways involved in seed primary physiological dormancy maintenance of Korean pine seed

Comparative Metabolome Analyses of Chinese Hickory Nut Kernels Highlight The Importance of Allocation Shift and Accumulation on Primary and Secondary Metabolites During In-Situ Germination

Analysis of the energy source at the early stage of poplar seed germination: verification of Perl’s pathway

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