Non-Targeted Ultra-High Performance Liquid Chromatography-High-Resolution Mass Spectrometry (UHPLC-HRMS) Fingerprints for the Chemometric Characterization and Classification of Turmeric and Curry Samples

Núñez, Oscar; Vidal-Casanella, Oscar; Sentellas, Sònia; Saurina, Javier; Núñez, Óscar

doi:10.3390/separations7020032

Cited by 6 publications

(5 citation statements)

References 36 publications

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“…There is significant variability within and between the cultivars of C. longa L. and C. aromatica Salisb, which can be exploited to differentiate the cultivars of Curcuma spp. The feasibility of studies without using any standard compounds was pointed out by Núñez et al (2020) . Similarly, reference compounds were not used in our study to derive arithmetic indices under the experimental conditions.…”

Section: Discussionmentioning

confidence: 99%

Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

et al. 2021

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Background:Curcuma spp. (Zingiberaceae) are used as a spice and coloring agent. Their rhizomes and essential oils are known for medicinal properties, besides their use in the flavoring and cosmetic industry. Most of these biological activities were attributed to volatile and nonvolatile secondary metabolites present in the rhizomes of Curcuma spp. The metabolite variations among the species and even cultivars need to be established for optimized use of Curcuma spp.Objectives: We compared the phytochemical profiles of rhizomes and their essential oils to establish the variability among seven cultivars: five of Curcuma longa L. (Alleppey Supreme, Duggirala Red, Prathibha, Salem, Suguna) and two of C. aromatica Salisb. (Kasturi Araku, Kasturi Avidi). The GC-MS and LC-MS-based analyses were employed to profile secondary metabolites of these selected cultivars.Methods: Rhizomes of Curcuma spp. were subjected to hydro-distillation to collect essential oil and analyzed by GC-MS. The methanol extracts of fresh rhizomes were subjected to LC-MS analyses. The compounds were identified by using the relevant MS library databases as many compounds as possible.Results: The essential oil content of the cultivars was in the range of 0.74–1.62%. Several compounds were detected from the essential oils and rhizome extracts by GC-MS and LC-MS, respectively. Of these, 28 compounds (13 from GCMS and 15 from LCMS) were common in all seven cultivars, e.g., α-thujene, and diarylheptanoids like curcumin. Furthermore, a total of 39 new compounds were identified from C. longa L. and/or C. aromatica Salisb., most of them being cultivar-specific. Of these compounds, 35 were detected by GC-MS analyses of essential oils, 1,2-cyclohexanediol, 1-methyl-4-(1-methylethyl)-, and santolina alcohol, to name a few. The other four compounds were detected by LC-MS of the methanolic extracts of the rhizomes, e.g., kaempferol-3,7-O-dimethyl ether and 5,7,8-trihydroxy-2′,5′-dimethoxy-3′,4′-methylene dioxyisoflavanone.Conclusions: We identified and recorded the variability in the metabolite profiles of essential oils and whole rhizome extracts from the seven cultivars of Curcuma longa L. and C. aromatica Salisb. As many as 39 new metabolites were detected in these seven Indian cultivars of Curcuma spp. Many of these compounds have health benefits.

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Section: Discussionmentioning

confidence: 99%

Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

et al. 2021

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“…With the same aim, Nuñez et al [7] used an untargeted approach by applying UHPLC high-resolution mass spectrometry (HRMS) to obtain the fingerprint of turmeric and curry samples in order to develop an efficient strategy to characterize, authenticate, and classify these two species. This strategy not only allowed an efficient metabolite profile to distinguish both species, but also to distinguish between the different varieties of each one.…”

Section: Special Issue Summarymentioning

confidence: 99%

Applications of Chromatographic Techniques in Food and Environmental Analysis

Socas‐Rodríguez

2022

Separations

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“…Chromatographic and related tandem techniques (or NMR spectroscopic analysis) have been applied as powerful tools for turmeric quality of DS products. − In this study, a strategy combining similarity analysis/chemometrics and ultrahigh-performance liquid chromatography–high-resolution mass spectrometry (UHPLC-HRMS) chemical analysis was used for evaluating turmeric DS quality based on their label claims. The curcuminoid labeling accuracy and the “natural” profile of the ten turmeric DSs were evaluated using the developed methodology.…”

Section: Introductionmentioning

confidence: 99%

Quality Evaluation of Turmeric Dietary Supplements in the United States Market Using a Quantification and UHPLC-HRMS Metabolite Ratio Approach

Liu,

Sun,

et al. 2023

ACS Food Sci. Technol.

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Turmeric is one of the most popular herbal botanicals used in the food and dietary supplement (DS) industries. As a DS, it faces labeling inconsistency and authenticity issues due to economic motivation. This research evaluated the labeling claim accuracy and chemical profile of commercial turmeric dietary supplemental products. Ten turmeric DSs from the US market along with one reference standard and two rhizome powders were analyzed using ultrahigh-performance liquid chromatography−highresolution mass spectrometry. Except 2 samples not given clear label facts, only 3 out of the 10 samples analyzed met the labeling expectation of curcuminoid amounts based on the sum of curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BMC) quantities. In addition, 13 known curcuminoids and volatile compounds were selected to represent the "natural" metabolite profile of turmeric, and the metabolite profiles of the commercial products were evaluated. Considering the addition of synthetic ingredients will break the "natural profile" ratio of turmeric, the ratios of curcuminoids/turmerones and ratios of CUR/DMC/BMC were selected as the quality markers after evaluating the metabolite profiles of turmeric rhizome samples and DSs. The natural turmeric (voucher sample and raw rhizome samples) has a ratio of less than 20% while some of the DS samples have ratios higher than 50% for turmerones/curcuminoids, and the curcumin ratio to curcuminoids is about 60 to 65% for the natural turmeric while some of the DS samples are well over 80%. The results suggest both the ratio of curcuminoids/turmerones and the ratio of CUR/DMC/BMC may be used as indicators for the quality markers of turmeric DSs.

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Non-Targeted Ultra-High Performance Liquid Chromatography-High-Resolution Mass Spectrometry (UHPLC-HRMS) Fingerprints for the Chemometric Characterization and Classification of Turmeric and Curry Samples

Cited by 6 publications

References 36 publications

Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

Applications of Chromatographic Techniques in Food and Environmental Analysis

Quality Evaluation of Turmeric Dietary Supplements in the United States Market Using a Quantification and UHPLC-HRMS Metabolite Ratio Approach

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