Non-thermal plasma treatment induces MAPK signaling in human monocytes

Bundscherer, Lena; Nagel, Stefanie; Hasse, Sybille; Tresp, Helena; Wende, Kristian; Walther, Reinhard; Reuter, Stephan; Weltmann, Klaus‐Dieter; Masur, Kai; Lindequist, Ulrike

doi:10.1515/chem-2015-0071

Cited by 22 publications

(24 citation statements)

References 39 publications

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“…Moreover, the longer the treatment time, the higher was the concentration of both species in the treated media and the reduction of cell viability for both monocytes and MDM. Similar findings have been reported for several cell lines [16,20,38].…”

Section: Discussionsupporting

confidence: 91%

“…As an example, Kaushik et al [18], reported the cytotoxic effects of CAP on monocytic lymphoma U937 cells and Bekeschus et al [19] demonstrated the differential sensitivity of blood mononuclear cell subpopulations to plasma treatment. Focusing on the effects of plasma on monocytes, the plasma treatment of primary human monocytes can activate the pro-proliferative or pro-apoptotic intracellular signaling cascades, depending on plasma treatment time [20]. Several papers have focused on the plasma treatment of macrophagic populations [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

Crestale

Laurita

Liguori

et al. 2018

Plasma

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Monocytes are involved in innate immune surveillance, establishment and resolution on inflammation, and can polarize versus M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages. The possibility to control and drive immune cells activity through plasma stimulation is therefore attractive. We focused on the effects induced by cold-atmospheric plasma on human primary monocytes and monocyte-derived macrophages. Monocytes resulted more susceptible than monocyte-derived macrophages to the plasma treatment as demonstrated by the increase in reactive oxygen (ROS) production and reduction of viability. Macrophages instead were not induced to produce ROS and presented a stable viability. Analysis of macrophage markers demonstrated a time-dependent decrease of the M1 population and a correspondent increase of M2 monocyte-derived macrophages (MDM). These findings suggest that plasma treatment may drive macrophage polarization towards an anti-inflammatory phenotype.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

Crestale

Laurita

Liguori

et al. 2018

Plasma

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“…Using transcriptomic profiling, we here wanted to extend on this by understanding the redox signaling responses and molecular pathways in Jurkat and THP‐1 cells following exposure to plasma‐treated medium. Previous experiments showed that several MAP kinases and chaperones were differentially regulated with 30 (Jurkat) and 75 s (THP‐1) treatment time compared to each respective control . Hence, we used these exposure times throughout for in depth transcriptomic analysis.…”

Section: Resultsmentioning

confidence: 99%

Redox‐regulation of activator protein 1 family members in blood cancer cell lines exposed to cold physical plasma‐treated medium

Schmidt

Rödder

Hasse

et al. 2016

Plasma Processes & Polymers

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Targeting cancer cells with cold physical plasma emerged as a promising application in plasma medicine. To understand its mode of action with regard to cellular redox‐regulation, two leukemia cell lines (Jurkat and THP‐1 cells) were plasma‐treated and their gene expression was compared. Transcriptomics revealed strikingly similar expression levels of activator protein 1 family members, and of a particular set of genes associated with apoptosis, DNA repair, and antioxidant defense. Interestingly, important constitutively expressed antioxidant transcripts were differently expressed in both cell types which suggest them to be crucial for the Jurkat's high and the THP‐1 cells’ low sensitivity to plasma. Utilizing these targets as biological read outs may help rendering plasmas more effective against different types of cancers.

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“…However, this was done in previous studies. Bundscherer and colleagues have shown that longer plasma treatment times, but not a concentration-matched amount of H 2 O 2, induces ERK-phosphorylation in primary monocytes [93]. Plasma-mediated ERK-phosphorylation was also found in THP-1 cells [94].…”

Section: Discussionmentioning

confidence: 99%

Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes

et al. 2019

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Background: Monocyte-derived macrophages are key regulators and producers of reactive oxygen and nitrogen species (ROS/RNS). Pre-clinical and clinical studies suggest that cold physical plasma may be beneficial in the treatment of inflammatory conditions via the release of ROS/RNS. However, it is unknown how plasma treatment affects monocytes and their differentiation profile. Methods: Naïve or phorbol-12-myristate-13-acetate (PMA)-pulsed THP-1 monocytes were exposed to cold physical plasma. The cells were analyzed regarding their metabolic activity as well as flow cytometry (analysis of viability, oxidation, surface marker expression and cytokine secretion) and high content imaging (quantitative analysis of morphology. Results: The plasma treatment affected THP-1 metabolisms, viability, and morphology. Furthermore, a significant modulation CD55, CD69, CD271 surface-expression and increase of inflammatory IL1β, IL6, IL8, and MCP1 secretion was observed upon plasma treatment. Distinct phenotypical changes in THP-1 cells arguing for a differentiation profile were validated in primary monocytes from donor blood. As a functional outcome, plasma-treated monocytes decreased the viability of co-cultured melanoma cells to a greater extent than their non-treated counterparts. Conclusions: Our results suggest plasma-derived ROS/RNS shaped a differentiation profile in human monocytes as evidenced by their increased inflammatory profile (surface marker and cytokines) as well as functional outcome (tumor toxicity).

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Non-thermal plasma treatment induces MAPK signaling in human monocytes

Cited by 22 publications

References 39 publications

Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

Redox‐regulation of activator protein 1 family members in blood cancer cell lines exposed to cold physical plasma‐treated medium

Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes

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