This study investigated the efficacy of ultraviolet-C (UV-C) irradiation in
enhancing the quality of raw bovine milk by targeting microbial populations and
lipid peroxidation, both of which are key factors in milk spoilage. We
categorized the raw milk samples into three groups based on initial bacterial
load: low (<3 Log 10 CFU/mL), medium (3–4 Log 10 CFU/mL), and high
(>4 Log 10 CFU/mL). Using a 144 W thin-film UV-C reactor, we treated the
milk with a flow rate of 3 L/min. We measured the bacterial count including
standard plate count, coliform count, coagulase-negative staphylococci count,
and lactic acid bacteria count and lipid peroxidation (via thiobarbituric acid
reactive substances assay) pre- and post-treatment. Our results show that UV-C
treatment significantly reduced bacterial counts, with the most notable
reductions observed in high and medium initial load samples (>4 and
3–4 Log 10 CFU/mL, respectively). The treatment was particularly
effective against coliforms, showing higher reduction efficiency compared to
coagulase-negative staphylococci and lactic acid bacteria. Notably, lipid
peroxidation in UV-C treated milk was significantly lower than in pasteurized or
untreated milk, even after 72 hours. These findings demonstrate the potential of
UV-C irradiation as a pre-treatment method for raw milk, offering substantial
reduction in microbial content and prevention of lipid peroxidation, thereby
enhancing milk quality.