Non-typhoidal Salmonella isolates from livestock and food samples, in and around Kolkata, India

Abstract

Objective: Non-typhoidal Salmonella (NTS) is a major foodborne pathogen in both developed and developing countries. The study was undertaken to determine the prevalence, serotype distribution and antimicrobial resistance profiles of NTS isolates from livestock and food samples collected in and around Kolkata during 2012-2013. Materials and methods:A total of 500 samples (animal, poultry and raw food) from 12 different places randomly collected in and around Kolkata were processed as per standard procedures and analyzed for NTS. Serotyping of the isolates was done followed by determination of antimicrobial resistance profiles and Minimum inhibitory concentrations following disc diffusion and E-test methods.Results: A total of 53 (10.6%) NTS isolates were recovered, with highest isolation from chicken (40%), followed by beef and poultry cloacal samples (22% each). Total 11 Salmonella serovars were identified with predominance of Salmonella enterica serovar Typhimurium (S. Typhimurium) (30.2%) followed by S. Kentucky (22.6%), S. Idikan (15.1%) and other serovars. This is the first report of isolation of S. Idikan from India. Thirty-two (60.4%) isolates were pan susceptible to all drugs tested. Percentage distribution of resistant NTS against antimicrobials were: nalidixic acid (24.5%), ampicillin (17%), amoxicillin (17%), streptomycin (15.1%), tetracycline (11.3%), chloramphenicol (9.4%), co-trimoxazole (9.4%) and ciprofloxacin (5.7%). Only one isolate (S. Senftenberg) was resistant to both fluroquinolones and third generation cephalosporins. Multidrug resistance (≥3 classes of antimicrobials) was observed in 13.2% NTS isolates. Conclusion:High prevalence of NTS in poultry and meat samples in Kolkata poses a potential risk of foodborne disease in humans. J Microbiol Infect Dis 2016;6(3): 113-120

Antimicrobial resistance, plasmid, virulence, multilocus sequence typing and pulsed-field gel electrophoresis profiles of Salmonella enterica serovar Typhimurium clinical and environmental isolates from India

“…We also included 16 environmental isolates of S . Typhimurium from our earlier study [29], for comparison with clinical isolates. The environmental isolates were recovered from poultry meat (n = 12), goat meat (n = 2), poultry cloacal swab (n = 1) and cattle rectal swab (n = 1) by random sampling of livestock and foods of animal origin for NTS in and around Kolkata during 2012–2013 [29].…”

“…Isolation of S . Typhimurium from humans, animals and food products has been frequently documented from the Indian subcontinent [19–29]. According to an article published from National Salmonella and Escherichia Centre, Kasauli, S .…”

“…Although AMR, plasmid and virulence profiles of S . Typhimurium isolates from India were reported in the past [15, 20–23,25–29], data on molecular subtypes of the strains were rare, which has public health importance with respect to tracking and controlling the transmission of the S . Typhimurium isolates.…”

Cloning, Sequencing and In Silico Characterization of OmpF Protein of Salmonella typhimurium for its Immune-Potential

“…Reports of Salmonella incidence from poultry stock of India indicate the possibility of transferring Salmonella from poultry to human populations where it can cause serious health issues (Kaushik et al, 2014;Sudhanthirakodi et al, 2016). Many strategic control programmes have been investigated to reduce the Salmonella burden from poultry flock.…”

High Prevalence of Metal Resistant Genes in <em>Salmonella enterica</em> MDR Plasmids Correlates Severe Toxicities of Water with higher Typhoid AMR

“…The genetic exchange and acquisition of mdr genes were happed in the gut and thus gut microbiome plaed a central role in shaping both mdr and metal resistant genes (Jain et al 2018). WGS of Salmonella has showed the existence of MDR-islands in Salmonella genome and thus virulence and multi-resistance will be more prominence in Salmonella infections (Saroj et al 2008;Liu et al 2009;Sudhanthirakodi et al 2016;Parkhill et al 2018;Luo et al 2020)…”