Non-uniformity in in vitro drug-induced cytotoxicity as evidenced by differences in IC50 values – implications and way forward

Femina, T. Arokia; Barghavi, V.; Archana, K.; Swethaa, N.G.; Ravi, Maddaly

doi:10.1016/j.vascn.2022.107238

Cited by 5 publications

(5 citation statements)

References 89 publications

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Section: Discussionmentioning

confidence: 67%

“…The phenomenon of the same drug differences in IC 50 cytotoxicity in the same cell line in vitro has already been described in previous studies [18,68]. Among the reasons for this diversity are cell density, cell culture time, and the method of cytotoxicity detection [68]. To investigate further the variability of published results, we analyzed IC 50 values of cisplatin in three human cancer cell lines (HeLa, HepG2 and MCF-7).…”

Section: Discussionmentioning

confidence: 84%

“…In a more recent work by Arokia Femina et al [68], the IC 50 values of 5-fluorouracil tests available in the literature on 10 types of human cancer cell lines (AGS (gastric adenocarcinoma), DLD1 and SNU-C4 (colorectal adenocarcinoma), HCT116 (colorectal carcinoma), HT-29 and MKN28 (gastric adenocarcinoma), MKN45 and SGC7901 (gastric carcinoma), SK-MES-1 (lung carcinoma), SW620 (colon adenocarcinoma)) were compared by using MTT, CCK-8, SRB, and a clonogenic assay in 12-72 h cultures with different cell densities. A wide scatter of the 5-fluorouracil IC 50 results (values 1.46-289.7 µM) was observed.…”

Section: Discussionmentioning

confidence: 95%

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Disparities in Cisplatin-Induced Cytotoxicity—A Meta-Analysis of Selected Cancer Cell Lines

Ćwiklińska-Jurkowska,

Wiese-Szadkowska,

Janciauskiene

et al. 2023

Molecules

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Cisplatin is a classic anticancer drug widely used as a reference drug to test new metal complex drug candidates. We found an unexpected diversity in cisplatin-related cytotoxicity values, expressed as IC50 (the half-maximal inhibitory concentration) in tumour cell lines, such as MCF-7, HepG2 and HeLa. We reviewed the data published from 2018 to 2022. A total of 41 articles based on 56 in vitro experiments met our eligibility criteria. Using a meta-analysis based on a random effect model, we evaluated the cytotoxicity of cisplatin (IC50) after 48- or 72-h cell exposure. We found large differences between studies using a particular cell line. According to the random effect model, the 95% confidence intervals for IC50 were extremely wide. The heterogeneity of cisplatin IC50, as measured by the I2 index for all cancer cell lines, was over 99.7% at culture times of 48 or 72 h. Therefore, the variability between studies is due to experimental heterogeneity rather than chance. Despite the higher IC50 values after 48 h than after 72 h, the heterogeneity between the two culture periods did not differ significantly. This indicates that the duration of cultivation is not the main cause of heterogeneity. Therefore, the available data is diverse and not useful as a reference. We discuss possible reasons for the IC50 heterogeneity and advise researchers to conduct preliminary testing before starting experiments and not to solely rely on the published data. We hope that this systematic meta-analysis will provide valuable information for researchers searching for new cancer drugs using cisplatin as a reference drug.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 95%

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Disparities in Cisplatin-Induced Cytotoxicity—A Meta-Analysis of Selected Cancer Cell Lines

Ćwiklińska-Jurkowska,

Wiese-Szadkowska,

Janciauskiene

et al. 2023

Molecules

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“…Similar cytotoxic effects were observed in Vero cells and NIH-3T3 cells . It is anticipated that the sensitivity of different cell lines to cytotoxic effects of the same compound will vary. − Despite this, the properties of CDP-1 and CDP-1D make them promising lead peptides, as they advance to the next stage of the development process.…”

Section: Resultsmentioning

confidence: 78%

A Snake Venom Peptide and Its Derivatives Prevent Aβ₄₂ Aggregation and Eliminate Toxic Aβ₄₂ Aggregates In Vitro

Camargo,

Gering,

Mastalipour

et al. 2024

ACS Chem. Neurosci.

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Over a century has passed since Alois Alzheimer first described Alzheimer's disease (AD), and since then, researchers have made significant strides in understanding its pathology. One key feature of AD is the presence of amyloid-β (Aβ) peptides, which form amyloid plaques, and therefore, it is a primary target for treatment studies. Naturally occurring peptides have garnered attention for their potential pharmacological benefits, particularly in the central nervous system. In this study, nine peptide derivatives of Crotamine, a polypeptide from Crotalus durissus terrificus Rattlesnake venom, as well as one D-enantiomer, were evaluated for their ability to modulate Aβ 42 aggregation through various assays such as ThT, QIAD, SPR, and sFIDA. All tested peptides were able to decrease Aβ 42 aggregation and eliminate Aβ 42 aggregates. Additionally, all of the peptides showed an affinity for Aβ 42 . This study is the first to describe the potential of crotamine derivative peptides against Aβ 42 aggregation and to identify a promising D-peptide that could be used as an effective pharmacological tool against AD in the future.

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“…Os dados obtidos no teste de MTT foram então utilizados para calcular a concentração inibitória média que diminuiu em 50% (IC50) a viabilidade celular de HCT-116 (Anexos A, B e C). O cálculo do IC50 é comumente utilizado para testar a citotoxicidade de moléculas e drogas em ensaios in vitro (FEMINA et al, 2023). Quanto menor o IC50, maior o efeito citotóxico da carragena modificada na viabilidade celular de HCT-116.…”

Section: Diminuição De Forma Dose-dependente Da Viabilidade Celular D...unclassified

Modificação química de polissacarídeos sulfatados para aumento da atividade anticâncer >i<in vitro>/i<

Lacerda

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Muitas pessoas fizeram parte dessa caminhada de mais de quatro anos, e eu tenho muito a agradecer! Aos meus pais, Shirley e Luiz, que são a minha base. Aconteça o que acontecer, mude o que for no mundo ou na vida, eles estão sempre lá, me incentivando, me acolhendo e acreditando em mim e em qualquer coisa que eu resolvo fazer. Eles foram e sempre serão a minha maior inspiração e a minha maior fonte de força e amor. Obrigada! Ao meu orientador, e amigo, Prof. João Paulo, o JP. A sua animação e o seu conhecimento pela ciência me inspiraram desde a primeira conversa. Mas ter a sua orientação e o seu companheirismo durante essa jornada foi o meu verdadeiro presente! Você vai além das portas científicas e sempre me acolheu com muito carinho nos momentos difíceis, fossem eles da pesquisa ou da vida. Sem o seu apoio teria sido infinitamente mais difícil tornar essa tese realidade. Obrigada! À Érika, a Pepa, minha amiga, minha irmã e minha companheira, que seguiu junto comigo por toda essa trajetória, por toda essa loucura, e mesmo não estando dentro do laboratório participou ativamente de tantos e tantos experimentos! Que sempre esteve com o abraço pronto, a prosa na ponta da língua e que nunca me deixou esquecer da minha força. Obrigada! Ao Rauni, meu companheiro e meu amor, que aguentou todas as minhas crises e minhas ausências, minhas mudanças de humor, minhas horas intermináveis no laboratório ou na frente do computador. Que me abasteceu com amor, abraços e comidinhas, e que esteve ao meu lado me lembrando que eu era capaz. Obrigada! Nesse caminho eu tive a sorte gigantesca de conhecer pessoas incríveis e de fazer amigos que permitiram que eu não enlouquecesse (completamente) durante todo esse processo. Obrigada por todos os cafés acolhedores, as brejas anestesiantes, as danças libertadoras, as conversas científicas, as fofocas, as escutas e as risadas! Eu vou arriscar escrever seus nomes, porque vocês são especiais demais, e sem vocês essa estadia no doutorado teria sido praticamente

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Non-uniformity in in vitro drug-induced cytotoxicity as evidenced by differences in IC50 values – implications and way forward

Cited by 5 publications

References 89 publications

Disparities in Cisplatin-Induced Cytotoxicity—A Meta-Analysis of Selected Cancer Cell Lines

Disparities in Cisplatin-Induced Cytotoxicity—A Meta-Analysis of Selected Cancer Cell Lines

A Snake Venom Peptide and Its Derivatives Prevent Aβ₄₂ Aggregation and Eliminate Toxic Aβ₄₂ Aggregates In Vitro

Modificação química de polissacarídeos sulfatados para aumento da atividade anticâncer >i<in vitro>/i<

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Non-uniformity in in vitro drug-induced cytotoxicity as evidenced by differences in IC50 values – implications and way forward

Cited by 5 publications

References 89 publications

Disparities in Cisplatin-Induced Cytotoxicity—A Meta-Analysis of Selected Cancer Cell Lines

Disparities in Cisplatin-Induced Cytotoxicity—A Meta-Analysis of Selected Cancer Cell Lines

A Snake Venom Peptide and Its Derivatives Prevent Aβ42 Aggregation and Eliminate Toxic Aβ42 Aggregates In Vitro

Modificação química de polissacarídeos sulfatados para aumento da atividade anticâncer >i<in vitro>/i<

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A Snake Venom Peptide and Its Derivatives Prevent Aβ₄₂ Aggregation and Eliminate Toxic Aβ₄₂ Aggregates In Vitro