Non-viral delivery of an optogenetic tool into cells with self-healing hydrogel

Hsieh, Fu-Yu; Han, Hao-Wei; Chen, Xiao Ru; Yang, Chii-Shen; Wei, Yen; Hsu, Shan‐hui

doi:10.1016/j.biomaterials.2018.05.014

Cited by 41 publications

(24 citation statements)

References 54 publications

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“…After treatment with proton ion pump proteins on A549 cells without light illumination, significant effects of growth inhibition on cancer cells by HEBR protein of the concentration of 40 μg/mL were observed. This is different from a previous study which demonstrated that HEBR-plasmid-transfected cells could pump out proton ion after green-light illumination [ 33 ]. No dramatic difference effects in the proliferation of A549 and H1299 cells was observed between the green-light group and the no-light group after the application of HEBR proteins.…”

Section: Discussioncontrasting

confidence: 99%

“…The HEBR protein is a light-trigger proton pump that has optogenetic effects to regulate the function of ion flow artificially, which has been used to investigate new photo-modulable treatments via specific lighting stimulation on neural cells in our previous reports [ 16 , 32 , 33 ]. Modulation of light-triggered proteins has been reported to develop novel treatments based on light-driven control of neural activity in many optogenetic experiments [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

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Engineered Bacteriorhodopsin May Induce Lung Cancer Cell Cycle Arrest and Suppress Their Proliferation and Migration

Wong

Huang

et al. 2021

Molecules

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Highly expressible bacteriorhodopsin (HEBR) is a light-triggered protein (optogenetic protein) that has seven transmembrane regions with retinal bound as their chromophore to sense light. HEBR has controllable photochemical properties and regulates activity on proton pumping. In this study, we generated HEBR protein and incubated with lung cancer cell lines (A549 and H1299) to evaluate if there was a growth-inhibitory effect with or without light illumination. The data revealed that the HEBR protein suppressed cell proliferation and induced the G0/G1 cell cycle arrest without light illumination. Moreover, the migration abilities of A549 and H1299 cells were reduced by ~17% and ~31% after incubation with HEBR (40 μg/mL) for 4 h. The Snail-1 gene expression level of the A549 cells was significantly downregulated by ~50% after the treatment of HEBR. In addition, HEBR significantly inhibited the gene expression of Sox-2 and Oct-4 in H1299 cells. These results suggested that the HEBR protein may inhibit cell proliferation and cell cycle progression of lung cancer cells, reduce their migration activity, and suppress some stemness-related genes. These findings also suggested the potential of HEBR protein to regulate the growth and migration of tumor cells, which may offer the possibility for an anticancer drug.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Engineered Bacteriorhodopsin May Induce Lung Cancer Cell Cycle Arrest and Suppress Their Proliferation and Migration

Wong

Huang

et al. 2021

Molecules

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“…In the application of central nervous system (CNS) repair, neurosphere-like progenitors showed better proliferation and differentiation in GC-DP hydrogel, and injection of GC-DP hydrogel combining neurospheres promoted functional recovery in a zebrafish CNS impaired model (Tseng et al, 2015 ). Moreover, the GC-DP hydrogel combining the optogenetic method was developed as a temporal-spatial approach to treat neurodegenerative diseases (Hsieh et al, 2018 ). The hydrogel containing bacteriorhodopsin plasmid and neural stem cells was injected into CNS impaired zebrafish where the neural repair was observed, particularly under green light exposure.…”

Section: Biomedical Applications Of Self-healing Hydrogelsmentioning

confidence: 99%

Synthesis and Biomedical Applications of Self-healing Hydrogels

2018

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Hydrogels, which are crosslinked polymer networks with high water contents and rheological solid-like properties, are attractive materials for biomedical applications. Self-healing hydrogels are particularly interesting because of their abilities to repair the structural damages and recover the original functions, similar to the healing of organism tissues. In addition, self-healing hydrogels with shear-thinning properties can be potentially used as the vehicles for drug/cell delivery or the bioinks for 3D printing by reversible sol-gel transitions. Therefore, self-healing hydrogels as biomedical materials have received a rapidly growing attention in recent years. In this paper, synthesis methods and repair mechanisms of self-healing hydrogels are reviewed. The biomedical applications of self-healing hydrogels are also described, with a focus on the potential therapeutic applications verified through in vivo experiments. The trends indicate that self-healing hydrogels with automatically reversible crosslinks may be further designed and developed for more advanced biomedical applications in the future.

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“…Specifically, hydrogels consist of hydrophilic polymer chains embedded in a hydrated 3D environment, which allows a homogenous entrapment of multi-cell types to replicate tissue complexities [ 41 , 42 ]. In addition, molecules in hydrogel networks can diffuse through the interconnected pores, which satisfies the requirements of vasculature for the delivery of molecules from vessels to tissues [ 43 , 44 ]. Nowadays, hydrogels are widely investigated and applied for clinical therapy.…”

Section: Introductionmentioning

confidence: 99%

Advances in hydrogel-based vascularized tissues for tissue repair and drug screening

Wang

Kankala

et al. 2022

Bioactive Materials

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The construction of biomimetic vasculatures within the artificial tissue models or organs is highly required for conveying nutrients, oxygen, and waste products, for improving the survival of engineered tissues in vitro . In recent times, the remarkable progress in utilizing hydrogels and understanding vascular biology have enabled the creation of three-dimensional (3D) tissues and organs composed of highly complex vascular systems. In this review, we give an emphasis on the utilization of hydrogels and their advantages in the vascularization of tissues. Initially, the significance of vascular elements and the regeneration mechanisms of vascularization, including angiogenesis and vasculogenesis, are briefly introduced. Further, we highlight the importance and advantages of hydrogels as artificial microenvironments in fabricating vascularized tissues or organs, in terms of tunable physical properties, high similarity in physiological environments, and alternative shaping mechanisms, among others. Furthermore, we discuss the utilization of such hydrogels-based vascularized tissues in various applications, including tissue regeneration, drug screening, and organ-on-chips. Finally, we put forward the key challenges, including multifunctionalities of hydrogels, selection of suitable cell phenotype, sophisticated engineering techniques, and clinical translation behind the development of the tissues with complex vasculatures towards their future development.

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Non-viral delivery of an optogenetic tool into cells with self-healing hydrogel

Cited by 41 publications

References 54 publications

Engineered Bacteriorhodopsin May Induce Lung Cancer Cell Cycle Arrest and Suppress Their Proliferation and Migration

Engineered Bacteriorhodopsin May Induce Lung Cancer Cell Cycle Arrest and Suppress Their Proliferation and Migration

Synthesis and Biomedical Applications of Self-healing Hydrogels

Advances in hydrogel-based vascularized tissues for tissue repair and drug screening

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