Nonaqueous and aqueous capillary electrophoresis of synthetic polymers

Cottet, Hervé; Simó, Carolina; Vayaboury, Willy; Cifuentes, Alejandro

doi:10.1016/j.chroma.2004.09.074

Cited by 44 publications

(53 citation statements)

References 94 publications

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“…As before, the electrophoretic mobility is determined by (2), leaving the task to estimate the effective charge from the simulation. There are several possible ways to determine the number of counterions that shield the bare charge of the polyelectrolyte.…”

Section: Estimating the Effective Chargementioning

confidence: 99%

“…charge, i.e. not all condensed counterions that are included in the new estimator Q (2) eff are taken into account.…”

Section: Estimating the Effective Chargementioning

confidence: 99%

“…Nowadays, electrophoretic separation methods are widely applied to study polyelectrolytes (PEs) such as proteins, DNA and synthetic polymers [1,2,3]. While there exist several theories [4,5,6,7] that have been successfully used to describe qualitatively the experimentally observed behaviour of various PEs, there are still many open problems to address.…”

Section: Introductionmentioning

confidence: 99%

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On the importance of hydrodynamic interactions in polyelectrolyte electrophoresis

Grass¹,

Holm²

2008

J. Phys.: Condens. Matter

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The effect of hydrodynamic interactions on the free-solution electrophoresis of polyelectrolytes is investigated with coarse-grained molecular dynamics simulations. By comparing the results to simulations with switched-off hydrodynamic interactions, we demonstrate their importance in modelling the experimentally observed behaviour. In order to quantify the hydrodynamic interactions between the polyelectrolyte and the solution, we present a novel way to estimate its effective charge. We obtain an effective friction that is different from the hydrodynamic friction obtained from diffusion measurements. This effective friction is used to explain the constant electrophoretic mobility for longer chains. To further emphasize the importance of hydrodynamic interactions, we apply the model to end-labeled free-solution electrophoresis.

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Section: Estimating the Effective Chargementioning

confidence: 99%

“…charge, i.e. not all condensed counterions that are included in the new estimator Q (2) eff are taken into account.…”

Section: Estimating the Effective Chargementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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On the importance of hydrodynamic interactions in polyelectrolyte electrophoresis

Grass¹,

Holm²

2008

J. Phys.: Condens. Matter

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“…The higher salt concentrations increase the number of counterions in the close vicinity of the chain as measured by Q (3) eff . At the same time, the electrostatic interactions in the system are reduced due to electrostatic screening, which also reduces the strength of the coupling between the polyelectrolyte and the counterions.…”

Section: Effective Chargementioning

confidence: 99%

“…Nowadays, electrophoresis methods are widely used to separate biomolecules [1,2] such as peptides, proteins, DNA, as well as synthetic polymers [3,4]. In order to be able to improve the processes involved in current electrophoretic separation methods it is a prerequisite to gain a thorough understanding of the behaviour of polyelectrolytes (PEs) in an externally applied electric field.…”

Section: Introductionmentioning

confidence: 99%

Mesoscale modelling of polyelectrolyteelectrophoresis

Grass

Holm

2010

Faraday Discuss.

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The electrophoretic behaviour of flexible polyelectrolyte chains ranging from single monomers up to long fragments of hundred repeat units is studied by a mesoscopic simulation approach. Abstracting from the atomistic details of the polyelectrolyte and the fluid, a coarse-grained molecular dynamics model connected to a mesoscopic fluid described by the Lattice Boltzmann approach is used to investigate free-solution electrophoresis. Our study demonstrates the importance of hydrodynamic interactions for the electrophoretic motion of polyelectrolytes and quantifies the influence of surrounding ions. The length-dependence of the electrophoretic mobility can be understood by evaluating the scaling behavior of the effective charge and the effective friction. The perfect agreement of our results with experimental measurements shows that all chemical details and fluid structure can be safely neglected, and a suitable coarse-grained approach can yield an accurate description of the physics of the problem, provided that electrostatic and hydrodynamic interactions between all entities in the system, i.e., the polyelectrolyte, dissociated counterions, additional salt and the solvent, are properly accounted for. Our model is able to bridge the single molecule regime of a few nm up to macromolecules with contour lengths of more than 100 nm, a length scale that is currently not accessible to atomistic simulations.

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Capillary and Microchip Electrophoresis

Thormann

2012

Ullmann's Encyclopedia of Industrial Chemistry

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Nonaqueous and aqueous capillary electrophoresis of synthetic polymers

Cited by 44 publications

References 94 publications

On the importance of hydrodynamic interactions in polyelectrolyte electrophoresis

On the importance of hydrodynamic interactions in polyelectrolyte electrophoresis

Mesoscale modelling of polyelectrolyteelectrophoresis

Capillary and Microchip Electrophoresis

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