Our safety and security are constantly being challenged. One of the vulnerabilities is that due to a bioterrorism attack. There are many targets and many bioweapons that are easily accessed. The continued advancements in technology that benefit mankind can also be exploited for nefarious acts. Forensic science can contribute to criminal interdiction or the investigative response after an attack. The field of microbial forensics has been developed to provide another avenue in the goal toward forensic attribution. Microbial forensics is the scientific discipline dedicated to analyzing evidence from a bioterrorism act, biocrime, hoax, or inadvertent microorganism/toxin release for attribution purposes. Microbial forensics combines the practices of epidemiology with the characterization of microbial and microbial‐related evidence to assist in determining the specific source of the sample, in ways that are as individualizing as possible, and/or the methods, means, processes and locations involved to determine the identity of the perpetrator(s) of an attack. Because of the impact of microbial forensic evidence high quality assurance and quality control practices must be in place and the limitations of any analysis properly understood.