2008
DOI: 10.1128/jvi.01917-07
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Noncanonical TATA Sequence in the UL44 Late Promoter of Human Cytomegalovirus Is Required for the Accumulation of Late Viral Transcripts

Abstract: During productive infection, human cytomegalovirus (HCMV) UL44 transcription initiates at three distinct start sites that are differentially regulated. Two of the start sites, the distal and the proximal, are active at early times, whereas the middle start site is active only at late times after infection. The UL44 early viral gene product is essential for viral DNA synthesis. The UL44 gene product from the late viral promoter affects primarily viral gene expression at late times after infection rather than vi… Show more

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Cited by 52 publications
(58 citation statements)
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“…To detect all transcripts derived from the different start sites, an RNase protection assay was performed as described in Materials and Methods. Consistent with previous reports (21,22,29), when the complementary cells were treated with PAA, the middle transcript was not detected (Fig. 6b, lane 8, and c, lane 6), as shown previously (21,22).…”
Section: Resultssupporting
confidence: 80%
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“…To detect all transcripts derived from the different start sites, an RNase protection assay was performed as described in Materials and Methods. Consistent with previous reports (21,22,29), when the complementary cells were treated with PAA, the middle transcript was not detected (Fig. 6b, lane 8, and c, lane 6), as shown previously (21,22).…”
Section: Resultssupporting
confidence: 80%
“…Each reaction mixture was described previously (23). HCMV MIE gene primers and reporter probes were described previously (19,21,23). Thermal cycling conditions and standard curve analysis were described previously (23).…”
Section: Cells and Virusesmentioning
confidence: 99%
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“…Homologous recombination was carried out in E. coli as described previously (33,34). To generate a deletion mutant BAC of BMRF1 gene, linear targeting constructs for recombination were prepared by PCR.…”
Section: Methodsmentioning
confidence: 99%
“…Similar differential promoter usage has also been reported for other viral genes. For example, transcription of UL44 of HCMV initiates at three distinct start sites that are differentially regulated (Isomura et al, 2008). The UL44 early viral promoters have a canonical TATA sequence, whilst the UL44 late viral promoter has a non-canonical TATA sequence used at the late stages of HCMV infection.…”
Section: Discussionmentioning
confidence: 99%