Noncoding RNAs in diagnosis and prognosis of graft‐versus‐host disease (GVHD)

Vajari, Mahdi Kohansal; Moradinasab, Susan; Yousefi, Amir‐Mohammad; Bashash, Davood

doi:10.1002/jcp.30830

Cited by 7 publications

(5 citation statements)

References 125 publications

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“…Several preclinical and clinical studies in various periods pre-and post-HSCT showed dysregulated miRNA levels and their function in massive immune responses leading to the development of GvHD [157,158]. A differently expressed miRNA profile appears to be a promising noninvasive tool for specific diagnosis and prediction of aGvHD occurrence (Figure 1).…”

Section: Microrna and Graft-versus-host Diseasementioning

confidence: 99%

Clinical Significance of microRNAs in Hematologic Malignancies and Hematopoietic Stem Cell Transplantation

Sevcikova

Fridrichová

Nikolaieva

et al. 2023

Cancers

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Hematologic malignancies are a group of neoplastic conditions that can develop from any stage of the hematopoiesis cascade. Small non-coding microRNAs (miRNAs) play a crucial role in the post-transcriptional regulation of gene expression. Mounting evidence highlights the role of miRNAs in malignant hematopoiesis via the regulation of oncogenes and tumor suppressors involved in proliferation, differentiation, and cell death. In this review, we provide current knowledge about dysregulated miRNA expression in the pathogenesis of hematological malignancies. We summarize data about the clinical utility of aberrant miRNA expression profiles in hematologic cancer patients and their associations with diagnosis, prognosis, and the monitoring of treatment response. Moreover, we will discuss the emerging role of miRNAs in hematopoietic stem cell transplantation (HSCT), and severe post-HSCT complications, such as graft-versus-host disease (GvHD). The therapeutical potential of the miRNA-based approach in hemato-oncology will be outlined, including studies with specific antagomiRs, mimetics, and circular RNAs (circRNAs). Since hematologic malignancies represent a full spectrum of disorders with different treatment paradigms and prognoses, the potential use of miRNAs as novel diagnostic and prognostic biomarkers might lead to improvements, resulting in a more accurate diagnosis and better patient outcomes.

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Section: Microrna and Graft-versus-host Diseasementioning

confidence: 99%

Clinical Significance of microRNAs in Hematologic Malignancies and Hematopoietic Stem Cell Transplantation

Sevcikova

Fridrichová

Nikolaieva

et al. 2023

Cancers

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“…The role of non-coding RNAs, mainly microRNAs, in T-cell immunobiology is well established and is increasingly being explored in the context of hematopoiesis and allogeneic HSCT ( 105 , 106 ). Several microRNAs are emerging as important regulators of allogeneic T-cells and may prove to be highly sensitive and specific biomarkers for GVHD and useful targets for anti-GVHD oligonucleotide-based therapeutics ( 105 – 108 ).…”

Section: Epigenetic Regulation Of Hematopoietic Stem Cells Before And...mentioning

confidence: 99%

The impact of epigenetic modifications on allogeneic hematopoietic stem cell transplantation

et al. 2023

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The field of epigenetics studies the complex processes that regulate gene expression without altering the DNA sequence itself. It is well established that epigenetic modifications are crucial to cellular homeostasis and differentiation and play a vital role in hematopoiesis and immunity. Epigenetic marks can be mitotically and/or meiotically heritable upon cell division, forming the basis of cellular memory, and have the potential to be reversed between cellular fate transitions. Hence, over the past decade, there has been increasing interest in the role that epigenetic modifications may have on the outcomes of allogeneic hematopoietic transplantation and growing enthusiasm in the therapeutic potential these pathways may hold. In this brief review, we provide a basic overview of the types of epigenetic modifications and their biological functions, summarizing the current literature with a focus on hematopoiesis and immunity specifically in the context of allogeneic hematopoietic stem cell transplantation.

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“…14 However, owing to the distinct microenvironments of various organs and tissues, the miRNAs associated with the disease also exhibit variations. 15,16 Consequently, it prompts the question of whether miRNA profiles also change in oral cGVHD patients and how these changes impact the disease.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of miRNA-769-5p and Smad2 in patients with or without oral cGVHD

Yong,

Zhou,

et al. 2024

Adv Clin Exp Med

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Background. Oral chronic graft-versus-host disease (cGVHD) impacts quality of life of patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, its precise pathogenesis remains unknown, with potential associations with differential microRNA (miRNA) expression and the TGF-β/Smad signaling pathway.Objectives. This study aims to explore miRNA expression profiles in the peripheral blood of oral cGVHD patients, focusing on miRNA-769-5p and its relationship with Smad2. Materials and methods.Peripheral venous blood samples were collected for RNA extraction from 8 patients with oral cGVHD, 8 patients without cGVHD and 8 participants from the healthy control group. The miRNA library was constructed using the Illumina Hiseq 2500 platform. We focused on identifying miRNAs associated with the TGF-β/Smad signaling pathway and subsequently conducted validation experiments. The oral cGVHD and without cGVHD groups were each expanded to include 15 individuals. Peripheral blood samples were subjected to polymerase chain reaction (PCR) analysis to assess miRNA levels and to evaluate Smad2 mRNA levels in peripheral blood mononuclear cells (PBMC). Additionally, enzyme-linked immunosorbent assay (ELISA) was conducted to determine the Smad2 protein levels in peripheral blood.Results. The most significantly differentially expressed miRNAs among the 3 groups were miRNA-505-5p and miRNA-769-5p. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated an enrichment of the target genes of miRNA-769-5p in the TGF-β signaling pathway. It was observed that miRNA-769-5p expression was higher in patients without oral cGVHD in comparison to those with oral cGVHD. Receiver operating characteristic (ROC) analysis demonstrated that miRNA-769-5p holds diagnostic value for oral cGVHD. As a target of miRNA-769-5p, Smad2 mRNA exhibited a negative correlation with it. Moreover, both Smad2 mRNA and protein levels were higher in patients with oral cGVHD as opposed to those without cGVHD.Conclusions. Differential expression of miRNAs, particularly the downregulation of miRNA-769-5p, may influence the development of oral cGVHD by diminishing its inhibitory effect on the TGF-β/Smad signaling pathway through its interaction with Smad2.

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Noncoding RNAs in diagnosis and prognosis of graft‐versus‐host disease (GVHD)

Cited by 7 publications

References 125 publications

Clinical Significance of microRNAs in Hematologic Malignancies and Hematopoietic Stem Cell Transplantation

Clinical Significance of microRNAs in Hematologic Malignancies and Hematopoietic Stem Cell Transplantation

The impact of epigenetic modifications on allogeneic hematopoietic stem cell transplantation

Differential expression of miRNA-769-5p and Smad2 in patients with or without oral cGVHD

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