Noncompetitive Immunodetection of Benzaldehyde by Open Sandwich ELISA

Shirasu, Naoto; Onodera, Takeshi; Nagatomo, Kazutaka; Shimohigashi, Yasuyuki; Toko, Kiyoshi; Matsumoto, Kiyoshi

doi:10.2116/analsci.25.1095

Cited by 16 publications

(8 citation statements)

References 19 publications

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“…Compared with different biosensors for benzadehyde detection, such as immunosensor, enzyme sensor and cell-based sensor (Badalyan et al , 2014; Gobi et al , 2007; Badalyan et al , 2013; Fiorentino et al , 2009; Shirasu et al , 2009), the OBPs-based biosensor showed relatively broad detection ranges. It suggested that the biosensor showed a relatively high sensitivity to benzaldehyde.…”

Section: Discussionmentioning

confidence: 99%

Olfactory biosensor based on odorant-binding proteins ofBactrocera dorsaliswith electrochemical impedance sensing for pest management

Yao

et al. 2017

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Purpose Using the remarkable olfaction ability, insects can sense trace amounts of host plant volatiles that are notorious for causing severe damage to fruits and vegetables and in consequence the industry. The purpose of the paper is to investigate the interactions between olfactory proteins, odorant-binding proteins (OBPs) and host plant volatiles through the developed olfactory biosensors. It might be helpful to develop novel pest control strategies. Design/methodology/approach Using the successfully expressed and purified OBPs of the oriental fruit fly Bactrocera dorsalis, a biosensor was developed by immobilizing the proteins on interdigitated electrodes through nitrocellulose membrane. Based on electrochemical impedance sensing, benzaldehyde emitted by the host plants, such as Beta vulgaris, was detected, which could be used to investigate and analyze the mechanisms of pests’ sense of chemical signals. The relative decreases of charge transfer resistances of the sensor were proportional to the odorant concentrations from 10−7 M to 10−3 M. Meanwhile, the interactions between OBPs and benzaldehyde were studied through the process of molecular docking. Findings The paper provides a pest OBPs-based biosensor that could sensitively detect the host odorants benzaldehyde. Meanwhile, the most related amino acids of OBPs that bind to host plant volatiles can be distinguished with molecular docking. Originality/value An olfactory biosensor was developed to explore interactions and mechanism between the pest OBPs and benzaldehyde, which showed promising potentials for small organic molecule sensing. Simultaneously, it might be helpful for novel pest control strategies.

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Section: Discussionmentioning

confidence: 99%

Olfactory biosensor based on odorant-binding proteins ofBactrocera dorsaliswith electrochemical impedance sensing for pest management

Yao

et al. 2017

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“…We can obtain antibodies against these fragrant compounds through the iliac lymph node method. Anti-fragrant monoclonal antibodies were developed [ 73 ], and an SPR measurement and open-sandwich enzyme-linked immunosorbent assay for benzaldehyde were carried out [ 74 , 75 ].…”

Section: Clues For Improvement Of Electronic Dog Nosementioning

confidence: 99%

Towards an Electronic Dog Nose: Surface Plasmon Resonance Immunosensor for Security and Safety

Onodera

Toko

2014

Sensors

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This review describes an “electronic dog nose” based on a surface plasmon resonance (SPR) sensor and an antigen–antibody interaction for security and safety. We have concentrated on developing appropriate sensor surfaces for the SPR sensor for practical use. The review covers different surface fabrications, which all include variations of a self-assembled monolayer containing oligo(ethylene glycol), dendrimer, and hydrophilic polymer. We have carried out detection of explosives using the sensor surfaces. For the SPR sensor to detect explosives, the vapor or particles of the target substances have to be dissolved in a liquid. Therefore, we also review the development of sampling processes for explosives, and a protocol for the measurement of explosives on the SPR sensor in the field. Additionally, sensing elements, which have the potential to be applied for the electronic dog nose, are described.

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“…Up to now, the OSIA system has been successfully applied in the detection of hapten molecules such as gibberellin (Lee et al, 2008), benzaldehyde (Shirasu et al, 2009), zearalenone (Suzuki et al, 2007), 4-hydroxy-3-nitrophenylacetyl (Yokozeki et al 2002), the carboxylterminal peptide of human osteocalcin (BGP) (Lim et al 2007), bisphenol A (Sakata et al 2009), and 11-Deoxycortisol ). This novel immunoassay system could be done in a shorter period of time than using a conventional sandwich assay, due to the omission of an incubation/washing cycle.…”

Section: Open Sandwich Immunoassay (Osia)mentioning

confidence: 99%

Recent Progress in Noncompetitive Hapten Immunoassays: A Review

Fan¹

2012

Trends in Immunolabelled and Related Techniques

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Noncompetitive Immunodetection of Benzaldehyde by Open Sandwich ELISA

Cited by 16 publications

References 19 publications

Olfactory biosensor based on odorant-binding proteins ofBactrocera dorsaliswith electrochemical impedance sensing for pest management

Olfactory biosensor based on odorant-binding proteins ofBactrocera dorsaliswith electrochemical impedance sensing for pest management

Towards an Electronic Dog Nose: Surface Plasmon Resonance Immunosensor for Security and Safety

Recent Progress in Noncompetitive Hapten Immunoassays: A Review

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