In this paper, an acoustic technique for air temperature and humidity measurement in moist air is described. The previous ultrasonic probe can enable the estimation of temperature from sound velocity in dry air by making use of the relationship between sound velocity and temperature. However, temperature measurement using the previous ultrasonic probe is not suitable in moist air because sound velocity also depends on humidity, and the temperature estimated from the sound velocity measured in moist air must be adjusted. Moreover, a method of humidity measurement by using only an ultrasonic probe has not been established. Thus, we focus on sound attenuation, which depends on temperature and humidity. Our proposed technique utilizes two parameters, sound velocity and attenuation, and can measure both temperature and humidity simultaneously. The acoustic technique for temperature and humidity measurement has the advantages that instantaneous temperature and humidity can be measured, and the measurement is not affected by thermal radiation because air itself is used as a sensing element. As an experiment, temperature and humidity are measured in a chamber, and compared with the reference values. The experimental results indicate the achievement of a practical temperature measurement accuracy of within ±0.5 K in moist air, of which the temperature is 293–308 K and relative humidity (RH) is 50–90% RH, and the simultaneous measurement of temperature and humidity.