Noncovalent functionalization of a nanofibrous network with a bio-inspired heavy metal binding peptide

Abstract

Peptide-polymer nanofibrous networks can be developed to obtain hybrid systems providing both functionalities of peptides and stability and processability of the polymers. In this work, a bio-inspired heavy metal binding peptide was synthesized and noncovalently immobilized on water-insoluble electrospun hydroxypropyl-beta-cyclodextrin nanofibers (CDNF). The peptide functionalized hybrid nanofibers were able to bind to heavy metal ions and facilitated removal of metal ions from water. The peptide-polymer scavenging system has potential for development of further molecular recognition systems with various peptide sequences or host-guest inclusion complexes.

In Situ functionalization of Poly(hydroxyethyl methacrylate) Cryogels with Oligopeptides via β-Cyclodextrin–Adamantane Complexation for Studying Cell-Instructive Peptide Environment

“…The Ad group was selected to anchor the RGD and GHK motif-based peptides (Figure ) to the cryogels because of its relative inertness and high affinity to β-CD with a binding constant in the range of 1 × 10 3 to 1 × 10 5 M –1 . , In the synthesized peptides, the Ahx-GG and Ahx-GGK­(probe)­G spacers were used , to ensure availability of the terminal bioactive sequence in the functionalized cryogels and to allow attachment of the fluorescent probes to the ε-amino group of lysine. The low β-sheet propensity of glycine was exploited in the spacers to separate the bioactive sequence or the bulky fluorescent probe.…”

Amphiphilic RGD and GHK peptides synergistically enhance liposomal delivery into cancer and endothelial cells

“…Glycine-based spacer was previously introduced to self-assembling bioactive peptide amphiphiles owing to its low intermolecular H-bonding and β-sheet propensity. 28,29…”

Bio‐Based Nanofibers Involved in Wastewater Treatment

“…e) Chemical structure and representative illustration of the peptide molecule and metal ions. Reproduced with permission . Copyright 2013, Royal Society of Chemistry.…”

“…The peptides are manually designed based on target of interest (heavy metals) to increase molecular recognition and scavenge metal ions from water. The PMP–CDNF network recovery increased by ≈1.6, 2.0, and 8.2 times (Cr(VI), Ni(II), and Cd(II)) greater than pristine‐CNDF . Immobilizing biosorbents such as Saccharomyces Cerevisiae onto PCL composite nanofibers by bio‐electrospraying could adsorb upto 238 mg g −1 of Pb(II) ions…”