Noncovalent Interactions of Tiopronin-Protected Gold Nanoparticles with DNA: Two Methods to Quantify Free Energy of Binding

The interactions of DNA, whether long, hundred base pair chains or short-chained oligonucleotides, with ligands play a key role in the field of structural biology. Its biological activity not only depends on the thermodynamic properties of DNA-ligand complexes, but can and often is conditioned by the formation kinetics of those complexes. On the other hand, gold nanoparticles have long been known to present excellent biocompatibility with biomolecules and are themselves remarkable for their structural, electronic, magnetic, optical and catalytic properties, radically different from those of their counterpart bulk materials, and which make them an important asset in multiple applications. Therefore, thermodynamic and kinetic studies of the interactions of DNA with nanoparticles acting as small ligands are key for a better understanding of those interactions to allow for their control and modulation and for the opening of new venues of research in nanomedicine, analytic and biologic fields. The interactions of gold nanoparticles with both DNA polymers and their smaller subunits; special focus is placed on those interactions taking place with nonfunctionalized gold nanoparticles are reviewed in the present work.

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Section: Interactions Between Aunps and Oligonucleotidesmentioning

confidence: 99%

Covalent and Non‐Covalent DNA–Gold‐Nanoparticle Interactions: New Avenues of Research

et al. 2016

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“…It can also be seen in the figure that the degree of compaction increases with the concentration of nanoparticles. As shown in this figures it was found that DNA for control differ than that for patients in their ability to aggregate and that was due to genetic differences between them where can be expected that the tiopronin nanoparticles binding occur principally through the formation of stable hydrogen bonding between the hydrophilic groups of the tiopronin and the DNA bases [12].…”

Section: Deoxyribonucleic Acid Extraction and Purificationmentioning

confidence: 79%

“…Effect of DNA Concentration: A different concentrations of DNA were used in order to study the effect of DNA concentration on binding with tiopronin gold nanoparticles (Tio Au NPs) by using different volume ratios of [12]. Effect of pH: A different pH values (2, 4, 6, 8, and10) of DNA/TioAuNPs solution were tested with increase concentration of DNA, and then the absorbance spectra by UV-Vis was measured to get a point intersection [13].…”

Section: Kinetic Studies Of Dna Binding With Tiopronin Gold Nanopartimentioning

confidence: 99%

“…Fluorescence Measurements: It is well known that small gold nanoparticles are able to emit fluorescence [22]. The change on the fluorescence spectra with increasing the amount of DNA which added to a solution containing a fixed gold nanoparticles concentration was observed [12] [22]. In previous studies of the emission from (Tio Au NPs), it was reported that the luminescence was (λ em =770) for crystalline size (1.8nm) and (λ em =656) for crystalline size (1.4nm), also they observed that the luminescence maximum shifts to lower energy with increasing core size [21].…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

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Thermodynamic and Kinetic Studies of Tiopronin Gold Nanoparticles Binding With Extracted DNA of Rheumatoid Arthritis

Abdulsattar¹,

Rashid²,

Hussain³

2018

MJS

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Nanomaterial can interact with biological systems in different way, using their surface functionalities and depending on the particle size, shape, and aggregation .The present work aims to characterize the binding of tiopronin gold nanoparticles with extracted DNA from blood patients with rheumatoid arthritis kinetically and thermodynamically. A total of 19 patients with rheumatoid arthritis attending Baghdad Teaching Hospital / Medical City were included in this study. Tiopronin monolayer-protected gold nanoparticles) were prepared by chemical methods and a partial purification of DNA extracted was preceded using negative ions exchange column chromatography. Characterization of Tio Au NPs binding with DNA was investigated by using TEM,SEM,FTIR,EDX and UV-VIS. techniques.The results indicated that the average size of Tio Au NPs was 48 nm.,and there is a different binding affinity of DNA to tiopronin gold nanoparticles depending on pH. Also the melting temperature of DNA was decreased 5C0 when binding with tiopronin gold nanoparticles. Thermodynamic studies indicated that the reaction was endothermic, less order, and non spontaneous.

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“…One example regards the non-covalent interactions between the biocompatible TPN-AuNP and double stranded DNA, which in its self plays a role in many applications. 17 Although gold complexants of low toxicity, including a number of thiolates, have been applied in gold plating, 18 TPN would also be an attractive alternative for forming gold structures in devices for in vivo use.…”

mentioning

confidence: 99%

Direct Gold Plating Selectively on UV Modified Polymer Film Using Tiopronin-Gold

Endo

Tashiro

et al. 2015

J. Electrochem. Soc.

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An economic and low toxicity gold plating process for bio-compatible device fabrication was investigated. A tiopronin-gold complex altered by mercaptothiodiazole or mercaptotriazole derivatives gave a form of the complex that could be reduced autocatalytically to enable electroless gold plating. Catalytic ca. 5 nm gold particles were selectively grafted into cycloolefin polymer and polyethylenenaphthalate film surfaces by adsorption of tiopronin-gold complex from solution into 20-30 nm deep ultra-violet light modified surface layers followed by reduction with sodium borohydride. Exposure of the catalyst adsorbed surfaces to baths composed of tiopronin-gold complex, mercaptothiodiazole and ascorbic acid resulted in selective electroless plating on the modified surfaces. Observation of the interface cross-section by transmission electron microscopy revealed that the gold deposits were physically anchored to the substrate by nanometer scale octopus trap like structures. Bio-compatible electronics for in vivo application represent an innovative field of medicine, offering ailment for life quality debilitating health complications. In the applications, sensors and transducers read or relay electronic input or output directly to or from living biological systems, like the brain. In retinal and cochlear neuroprosthesis, neural array implants facilitate direct stimulation of optic nerves with signals from a miniature camera, or direct stimulation of cochlear nerves with signals from a microphone. Research involving conscious neural control to operate external devices like prosthetic robots has also been undertaken. Neural electrode arrays also serve in therapeutic stimulation, bypass or diagnosis of specific neural systems in stroke and head injury patients and in patients suffering from Parkinson's disease, epilepsy, dystonia and severe clinical depression.1-10 Another important application for in vivo electronics is sensors and MEMS on catheters, allowing sensory feedback to the operator or manipulation of a MEMS device internally.11,12 Micro in vivo electronic sensors also offer a means of less invasive direct monitoring by wireless transmission or aid efficient pharmaceutical release. 13Non-toxic, non-allergenic and inert gold and platinum can be used as electrode materials; however toxins and allergens should be avoided in fabrication. Economic plating techniques that rely on bath components and construction materials that are toxic or allergenic introduce health risks and are therefore difficult to implement in fabrication. One approach for deposition of biocompatible gold patterns on poly(dimethylsiloxane) has been described using chemical plating and electrochemical etching. 14 In medicine, tiopronin (TPN) has been used therapeutically as a treatment of cystine disulfide related ailments like cystinuria and prophylaxis of renal cystine calculi, functioning based on an increase in solubility. 15,16 Tiopronin capped gold nanoclusters/nanoparticles (TPN-AuNP) have also been a center of attention in a broad spectrum...

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Noncovalent Interactions of Tiopronin-Protected Gold Nanoparticles with DNA: Two Methods to Quantify Free Energy of Binding

Cited by 6 publications

References 47 publications

Covalent and Non‐Covalent DNA–Gold‐Nanoparticle Interactions: New Avenues of Research

Covalent and Non‐Covalent DNA–Gold‐Nanoparticle Interactions: New Avenues of Research

Thermodynamic and Kinetic Studies of Tiopronin Gold Nanoparticles Binding With Extracted DNA of Rheumatoid Arthritis

Direct Gold Plating Selectively on UV Modified Polymer Film Using Tiopronin-Gold

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