Noncovalent PEGylation via Lectin–Glycopolymer Interactions

Abstract

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How Low Can You Go? Low Densities of Poly(ethylene glycol) Surfactants Attract Stealth Proteins

“…One of the first reports addressing the adsorption of PEG chains by Malmsten et al has shown, however, that the protein rejecting capacity of physically adsorbed PEG chains is similar to the efficient protein rejection at sufficiently high chain density of covalently grafted PEG layers. [18] It is worth mentioning that theoretical approaches to describe the effect of modulating size and grafting density of PEG on the plasma adsorption behavior of PEGylated surfaces are in concordance with experimental studies. In general, this method would allow for incorporation of glycol-PEG functionalities in proteins with an engineered sugar-binding site.…”

Associative and Dissociative Processes in Non‐Covalent Polymer‐Mediated Intracellular Protein Delivery

“…Non-covalent polymer-protein binding studies, sometimes involvingd elivery experiments, is ab road field, and additional reports relatedt ot his topic that highlight various binding interactions and characterization methods can be found in Refs. [58][59][60][61][62][63][64][65][66][67][68][69][70][71].B inding studies showcasing availablet echniques could potentially contain criticali nformation necessary for future protein-delivery optimization studies and should not be overlooked.…”

Photo-responsive polymeric micelles and prodrugs: synthesis and characterization

“…Although the exact mechanism of this interaction remains unknown, numerous studies have demonstrated that the mechanism is highly specic and noncovalent. 34 Thus, an in vitro evaluation of this specic binding event provides an initial test of the ability of a synthetic Gluco-ONB-P(aN 3 CL-g-PONBIMC) 10 polymer to interact with the biological systems, such as in drug delivery development, tissue engineering, and biomedical material synthesis. These tests are typically conducted by mixing Gluco-ONB-P(aN 3 CL-g-PONBIMC) 10 with a lectin that is selective for the sugar conjugated to the polymer.…”