Nondestructive and rapid method for sex identification of the tropical sea cucumber Holothuria scabra by anal swab sampling

Zixuan, E; Cheng, Chuhang; Wu, Feifei; Ren, Chen; Chen, Rong; Rao, Ying-Zhu; Ma, Bo; Jiang, Xiao; Luo, Peng; Li, Xiaomin; Zhang, Xin; Jiang, Fajun; Hu, Chaoqun; Chen, Ting

doi:10.1016/j.aquaculture.2022.738749

Cited by 7 publications

(2 citation statements)

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“…And the sex determination of holothurians is still ambiguous. Previously, we have investigated the metabolomics profiles and sex makers of two sexes(36, 37). In this study, to enrich our knowledge of sex difference of H. scabra , the comparative proteomics between ovary and testis was performed using label-free quantitative method.…”

Section: Discussionmentioning

confidence: 99%

Proteome analysis provides insights into sex differences inHolothuria Scabra

Cheng,

Wu,

et al. 2024

Preprint

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Sex-determining mechanism is still ambiguous for sea cucumber Holothuria scabra which only manifests gonochorism in gonad. In this study, proteomic analysis was employed to delineate sex-related proteins and genes in gonads of H. scabra, subsequently validated through Quantitative real-time polymerase chain reaction (qRT-PCR). A total of 5,313 proteins were identified via proteome sequencing. Among these, 817 proteins exhibited expression in both the ovary and testis, with 445 proteins displaying up-regulation and 372 proteins showing down-regulation. Furthermore, 136 and 69 proteins were identified as ovary-specific and testis-specific Differentially Abundant Proteins (DAPs), respectively. For the validation of 75 DAP coding genes, 9 genes were considered to be reliable. Notably, 25 ovary-bias proteins enriched in ribosome pathway strongly indicated the crucial role of ribosome in ovary. And 5S/18S rRNA ratio in H. Scabra may have potencial to establish a nondestructive method to distinguish sexes unambiguously. This study serves to furnish novel evidence pertaining to sex differences in H. scabra.

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Section: Discussionmentioning

confidence: 99%

Proteome analysis provides insights into sex differences inHolothuria Scabra

Cheng,

Wu,

et al. 2024

Preprint

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“…According to the A.japonicus genome sequences (taxid: 307972), the full-length cDNA sequences of AjSRα have been obtained by 5’ and 3’ rapid amplification of cDNA ends (RACE). The primers used in this study were designed on the software of Primer Premier 5 (Table S1 ) 49 , 50 . The amino acid sequence of AjSRα deduced and conserved structural domains predicted were performed with open reading frame (ORF) Finder and SMART program.…”

Section: Methodsmentioning

confidence: 99%

A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas

Zhang,

Sun,

et al. 2023

Sci Rep

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The signal recognition particle (SRP) system delivers approximately 30% of the proteome to the endoplasmic reticulum (ER) membrane. SRP receptor alpha (SRα) binds to SRP for targeting nascent secreted proteins to the ER membrane in eukaryotic cells. In this study, the SRα homologous gene was identified in the sea cucumber, Apostichopus japonicus (AjSRα). AjSRα codes for 641 amino acids and has 54.94% identity with its mammalian homologs. Like mammalian SRα, it is expected to contain the SRP-alpha N domain, SRP54_N domain, and SRP54 domain. In addition, AjSRα is ubiquitously expressed in adult tissues and exhibits a sexually dimorphic expression pattern, with significantly higher expression in ovaries compared to testes. As a maternal factor, AjSRα can be continuously detected during embryonic development. Importantly, we first attempted to investigate its function by using lentiviral vectors for delivering SRα gene-specific shRNA, and we revealed that lentiviral vectors do not induce an upregulation of immune-related enzymes in sea cucumbers. However, compared to the dsRNA-based RNA interference (RNAi) method, lentivirus-mediated RNAi caused dynamic changes in gene expression at a later time. This study supplied the technical support for studying the functional mechanism of SRα in sea cucumbers.

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Applications of Environmental DNA (eDNA) in Monitoring the Endangered Status and Evaluating the Stock Enhancement Effect of Tropical Sea Cucumber Holothuria Scabra

Luo,

Ren

et al. 2023

Mar Biotechnol

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Nondestructive and rapid method for sex identification of the tropical sea cucumber Holothuria scabra by anal swab sampling

Cited by 7 publications

References 50 publications

Proteome analysis provides insights into sex differences inHolothuria Scabra

Proteome analysis provides insights into sex differences inHolothuria Scabra

A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas

Applications of Environmental DNA (eDNA) in Monitoring the Endangered Status and Evaluating the Stock Enhancement Effect of Tropical Sea Cucumber Holothuria Scabra

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