Nondigestible carbohydrates, butyrate, and butyrate-producing bacteria

Fu, Xiaodan; Liu, Zhemin; Zhu, Chunshen; Mou, Haijin; Kong, Qing

doi:10.1080/10408398.2018.1542587

Cited by 360 publications

(229 citation statements)

References 236 publications

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“…Analysis by isolate according to the lineage identified () showed that the isolates of oriental origin (lineage-III) codify a greater number of AMRg than the other geographical origins. These results are of importance, because these types of genes are determinants for metabolic processes that lead to the production of SCFAs during glucose fermentation [12], mainly butyric acid [71]. In the case of the patient studied in this work, the possible immunosuppression caused as a consequence of the anti-inflammatory effect of prednisone (corticosteroid used for the treatment of rheumatoid arthritis) [60] prescribed for idiopathic rheumatoid arthritis may have been balanced by: healthy lifestyle habits and/or the consumption of substances with potential restorative effects on the gut microbiota such as Chlorella (microalgae consumed by the patient because has potential as an antioxidant and in treatment of different health conditions) [61].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive genome analyses of Sellimonas intestinalis, a potential biomarker of homeostasis gut recovery

et al. 2020

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Sellimonas intestinalis is a Gram-positive and anaerobic bacterial species previously considered as uncultivable. Although little is known about this Lachnospiraceae family member, its increased abundance has been reported in patients who have recovered from intestinal homeostasis after dysbiosis events. In this context, the aim of the present study was to take advantage of a massive in vitro culture protocol that allowed the recovery of extremely oxygen-sensitive species from faecal samples, which led to isolation of S. intestinalis . Whole genome analyses of 11 S . intestinalis genomes revealed that this species has a highly conserved genome with 99.7 % 16S rRNA gene sequence similarity, average nucleotide polymorphism results >95, and 50.1 % of its coding potential being part of the core genome. Despite this, the variable portion of its genome was informative enough to reveal the existence of three lineages (lineage-I including isolates from Chile and France, lineage-II from South Korea and Finland, and lineage-III from China and one isolate from the USA) and evidence of some recombination signals. The identification of a cluster of orthologous groups revealed a high number of genes involved in metabolism, including amino acid and carbohydrate transport as well as energy production and conversion, which matches with the metabolic profile previously reported for microbiota from healthy individuals. Additionally, virulence factors and antimicrobial resistance genes were found (mainly in lineage-III), which could favour their survival during antibiotic-induced dysbiosis. These findings provide the basis of knowledge about the potential of S. intestinalis as a bioindicator of intestinal homeostasis recovery and contribute to advancing the characterization of gut microbiota members with beneficial potential.

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Section: Discussionmentioning

confidence: 99%

Comprehensive genome analyses of Sellimonas intestinalis, a potential biomarker of homeostasis gut recovery

et al. 2020

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“…The individual analyzes grouped by the detected populations, showed that the isolates of oriental origin (linage-III), codify a greater number of genes of AMRg than the other geographical origins. These results are of importance, because these types of genes are determinants for the realization of metabolic processes that lead to the production of short-chain fatty acid (SCFA) during glucose fermentation, 10 mainly butyric acid, 31 which It has been proposed as one of the characteristics of the candidate members for healthy microbiota. 11 In the specific case of the patient from whom the isolate studied in this work was obtained despite the immunosuppression caused as a possible consequence of the anti-inflamatory effect of Prednisone (the corticosteroid consumed as treatment of rheumatoid arthritis) 20 , the healthy lifestyle habits and the consumption of substances with potential restorative effect of the gut microbiota as Chlorella (the microalgae consumed by the donor individual because has shown potential as antioxidant and treatment for different health conditions) 21 , they could contribute to the restored effect of the Microbiota, and the presence of S. intestinallis could then be complying with the hypothesis as a biomarker of the recovery of intestinal homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive genome analyses ofSellimonas intestinalis, a potential biomarker of homeostasis gut recovery

Muñoz

Guerrero-Araya²,

Cortés-Tapia³

et al. 2020

Preprint

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Comprehensive genome analyses of Sellimonas intestinalis, a potential 19 biomarker of homeostasis gut recovery 20 Sellimonas intestinalis is a Gram positive and anaerobic bacterial species 21 previously considered as uncultivable. Although little is known about this 22 Lachnospiraceae family member, its increased abundance has been reported in 23 patients who recovered intestinal homeostasis after dysbiosis events. In this 24 context, the aim of this work was taken advantage of a culturomics protocol that 25 allowed the recovery species extremely oxygen-sensitive from faecal samples, 26 which led to the establishment of an S. intestinalis isolate. Whole genome 27 sequencing and taxonomic allocation confirmation were the base to develop 28 comparative analyses including 11 public genomes closely related. 29 Phylogeographic analysis revealed the existence of three lineages (linage-I 30 including isolates from Chile and France, linage-II from South Korea and Finland, 31 and linage-III from China and one isolate from USA). Pangenome analysis on the 32 established dataset revealed that although S. intestinalis seems to have a highly 33 conserved genome (with 50.1% of its coding potential being part of the 34 coregenome), some recombination signals were evidenced. The identification of 35 cluster of orthologous groups revealed a high number of genes involved in 36 metabolism, including amino acid and carbohydrate transport as well as energy 37 production and conversion, which matches with the metabolic profile previously 38 reported for healthy microbiota. Additionally, virulence factors and antimicrobial 39 resistance genes were found (mainly in linage-III), which could favour their 40 survival during antibiotic-induced dysbiosis. These findings provide the basis of 41 knowledge about this species with potential as a bioindicator of intestinal 42 homeostasis recovery and contribute to advance in the characterization of gut 43 microbiota members with beneficial potential.44

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“…These metabolic end products, especially the fermentation product butyrate, can enhance the immune function of intestinal mucosa (31,32). In a cohort study, increased bacterial abundance in Blautia was closely associated with decreased lethal acute graft-versus-host disease (GVHD) and improved overall survival, suggesting that Blautia may result in suppressing immune rejection (33). Blautia is part of the most important markers to improve the prognosis of various clinical diseases such as visceral fat accumulation(34) and Crohn's disease (35).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Gut Microbiome and Prediction of Infection in Neutropenic Children with Acute Leukemia

Wang

et al. 2020

Preprint

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Background: Neutropenia in children with acute leukemia have a high incidence of infection and mortality. To identify and classify the potentially infected pathogens, this study compared the structural characteristics of gut microbiome in neutropenic and non-neutropenic children with acute leukemia. Results: The results showed that 6033 OUTs were observed in total, and the sequence coverage index was more than 0.97. In the analysis of alpha diversity, the colony richness index (Chao1 index) of Group A1 was significantly lower than that of Group A0 (P = 0.035). The fecal bacterial communities were dominated by the phylum Firmicutes, Proteobacteria, and Bacteroidetes in both groups, with no significant difference. Higher relative abundance of genera Enterococcus (P = 0.0076), Streptococcus (P = 0.014) and species Bacteroides fragilis (P = 0.034) were observed in Group A1, but class Clostridia (P = 0.038), genera Blautia (P = 0.021) and Roseburia (P = 0.011) were more prevalent in Group A0. The relatively high abundance of Bacteroides fragilis in neutropenia with childhood acute leukemia was an independent risk factor for infection (P=0.028, 95% CI 1.024-1.241).Conclusions: The increase of Enterococcus, Streptococcus and Bacteroides fragilis, and the decrease of Clostridium, Blautia, and Roseburia may be the characteristics of intestinal flora in patients with acute leukemia. The relatively high abundance of Bacteroides fragilis in neutropenia with childhood acute leukemia may predict the occurrence of infection.

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Nondigestible carbohydrates, butyrate, and butyrate-producing bacteria

Cited by 360 publications

References 236 publications

Comprehensive genome analyses of Sellimonas intestinalis, a potential biomarker of homeostasis gut recovery

Comprehensive genome analyses of Sellimonas intestinalis, a potential biomarker of homeostasis gut recovery

Comprehensive genome analyses ofSellimonas intestinalis, a potential biomarker of homeostasis gut recovery

Characteristics of Gut Microbiome and Prediction of Infection in Neutropenic Children with Acute Leukemia

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