2009
DOI: 10.1002/pro.37
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Nonenzymatic biotinylation of histone H2A

Abstract: Holocarboxylase synthetase (HCS, eukaryotic enzyme) and BirA (prokaryotic) are biotin protein ligases that catalyze the ATP-dependent attachment of biotin to apocarboxylases via the reactive intermediate, bio-5 0 -AMP. In this study, we examined the in vitro mechanism of biotin attachment to histone H2A in the presence of HCS and BirA. The experiment derives from our observations that HCS is found in the nucleus of cells in addition to the cytoplasm, and it has the ability to attach biotin to histones in vitro… Show more

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Cited by 26 publications
(25 citation statements)
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“…Using high resolution Orbitrap scans and HCD fragmentation data at 28% NCE, the computation successfully identified well described diagnostic ions for e.g. lysine acetylation (measured at 126.0914 m/z, error to theoretical mass 0.3 ppm; Figure 5a) lysine formylation (measured at 112.0758 m/z, mass error 0.7 ppm), lysine biotinylation (measured at 310.1584 m/z, mass error 0.1 ppm) (35,38,39) and tyrosine phosphorylation (measured at 216.0418 m/z, mass error 0.9 ppm) (36,40). We therefore sought to identify new features for other PTMs within our library.…”
Section: Downloaded Frommentioning
confidence: 83%
“…Using high resolution Orbitrap scans and HCD fragmentation data at 28% NCE, the computation successfully identified well described diagnostic ions for e.g. lysine acetylation (measured at 126.0914 m/z, error to theoretical mass 0.3 ppm; Figure 5a) lysine formylation (measured at 112.0758 m/z, mass error 0.7 ppm), lysine biotinylation (measured at 310.1584 m/z, mass error 0.1 ppm) (35,38,39) and tyrosine phosphorylation (measured at 216.0418 m/z, mass error 0.9 ppm) (36,40). We therefore sought to identify new features for other PTMs within our library.…”
Section: Downloaded Frommentioning
confidence: 83%
“…Although being relatively rare (37), biotinylated histones have been detected in human cells and play a functional role in gene silencing, responses to DNA damage, and cell proliferation (38). Spontaneous histone biotinylation occurs in the presence of biotinyl-59-AMP and in the absence of biotin protein ligases (39). Thus, an increased intracellular biotin content and/or turnover can elevate the amount of biotinylated histones.…”
Section: Discussionmentioning
confidence: 99%
“…Modifications of histone H3: H3R2me [22,15,171], H3R2cit [37], H3T3ph [22], H3K4me [22,60], H3K4ac [60], H3K4bio [90], H3R8me [22], H3R8cit [37], H3K9ac [22,60], H3K9bio [90], H3K9me [22,60] functional information on non-enzymatic histone biotinylation [70], we excluded these marks from further calculations.…”
Section: Information On Dna Versus Nucleosomesmentioning
confidence: 99%