Nonfaradaic Impedance Probing of Potato Glycoalkaloids Interaction with Butyrylcholinesterase Immobilized onto Gold Electrode

Abstract

In this paper, we report the development of enzyme-based nonfaradaic impedimetric biosensor for direct detection of potato glycoalkaloids a-chaconine and a-solanine. The sensors were prepared by cross-linking of horse butyrylcholinesterase (BuChE) onto gold electrodes. Affinity interaction of both glycoalkaloids with immobilized BuChE caused an essential decrease of the total impedance and of the interfacial polarization resistance as well. No effect was observed after a-chaconine contact with bovine serum albumin and bacterial carboxylesterase immobilized onto gold. BuChE-modified electrodes were more sensitive to a-chaconine, and found I 50 values were in quite good agreement with those measured by biocatalytic ISFET-based sensors.

Immittance Electroanalysis in Diagnostics

“…a generic input/output signal) beyond traditional EIS approaches 11,19,[32][33][34][35] and specifically none which do not require a premodelling of any given interface of interest. We show herein, that, in applying the transfer function concept to EIS datasets it is not only possible to extract analytically potent information but to also automate this without being limited to the confines of a specific physical model.…”

“…Despite the large number of reported impedance works, there has been no systematic quantitative consideration of EIS as a generic electrical transfer function (i.e., a generic input/output signal) beyond traditional EIS approaches ,,− and specifically none which do not require a premodeling of any given interface of interest. We show herein that, in applying the transfer function concept to EIS data sets, it is possible not only to extract analytically potent information but also to automate this without being limited to the confines of a specific physical model.…”

Electrochemical Determination of Glycoalkaloids Using a Carbon Nanotubes-Phenylboronic Acid Modified Glassy Carbon Electrode

“…The linear range was wider compared to using the impedance probe method [11]. The corresponding linear function was I P (μA) = 50.541 + 0.810 C α-solanine (mM) with a correlation coefficient of R = 0.9991.…”

“…It was noted that the peak current of α-solanine increased linearly with the increase of the α-solanine concentration from 1 to 28 μM ( Figure 4B ). The linear range was wider compared to using the impedance probe method [ 11 ]. The corresponding linear function was I P (μA) = 50.541 + 0.810 C α-solanine (mM) with a correlation coefficient of R = 0.9991.…”

“…Several methods have been used to detect GAs, such as High Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS) [ 8 , 9 ]. Some enzyme-based biosensors were developed for detection of GAs, such as butyrylcholinesterase biosensors based on pHISFETs and non-Faradaic impedimetric biosensors [ 10 , 11 ]. Among them, the most widely used one is HPLC, a chromatographic technique for separation, identification and quantification of compounds in a mixture.…”