Nongenetic Approach for Imaging Protein Dimerization by Aptamer Recognition and Proximity-Induced DNA Assembly

Abstract

Herein, we report a nongenetic and real-time approach for imaging protein dimerization on living cell surfaces by aptamer recognition and proximity-induced DNA assembly. We use the aptamer specific for the receptor monomer as a recognition probe. When receptor dimerization occurs, the dimeric receptors bring two aptamer probes into close proximity, thereby triggering dynamic DNA assembly. The proposed approach was successfully applied to visualize dimerization of Met receptor and transforming growth factor-β type II receptor. This approach allows us to image the two states (monomer/dimer) of a receptor protein on living cell surfaces in real time, opening a universal method for further investigation of protein dimerization and the corresponding activation processes in signal transduction.

Logic‐Gate‐Actuated DNA‐Controlled Receptor Assembly for the Programmable Modulation of Cellular Signal Transduction

“…[19] Furthermore,b ased on aptamer recognition and proximity-induced DNAa ssembly,o ur group recently reported an ongenetic approach to investigate receptor dimerization, which is often postulated to be the initial step for activating intracellular signal transduction. [20] Typically,c ellular signal transduction is predominantly based on protein interactions, which enable af ast response to input signals. [21] At the nanoscale,the spatial organization of cell surface receptors on living cell membrane is crucial for controlling cellular signal transduction.…”

Engineering Cell‐Surface Receptors with DNA Nanotechnology for Cell Manipulation

“…Moreover, DNA‐mediated receptor engineering has also been exploited for monitoring receptor dimerization induced by natural ligands. Through using a combination of RTK‐binding DNA aptamers and proximity‐induced DNA assembly, natural ligand‐induced RTK activation on the cell surface could be visualized through real‐time monitoring of the monomer or dimer forms of RTKs (e.g., c‐Met and TβRII) with use of specific fluorescent‐labeled DNA probes …”

“…Through using ac ombinationo f RTK-binding DNA aptamers and proximity-inducedD NA assembly,n atural ligand-inducedR TK activation on the cell sur-face could be visualized through real-time monitoring of the monomer or dimer forms of RTKs (e.g.,c -Met and TbRII) with use of specific fluorescent-labeled DNA probes. [61] In addition to mimicking natural ligand-trigger receptora ctivation, RTKs could be engineered with designed DNA nanodevices to reprogram cells to respond to noncognate ligands with artificially promoted cell migration. The Sando group de-signedaD NA aptamer-mediated reprogramming of the interaction partner of receptor tyrosine kinases (DRIPaR) strategy to "rewire" RTK-mediateds ignaling ( Figure 4B).…”

A selective sulphur‐containing colorimetric chemosensor for Cu2+

“…There are various methods for copper detection, such as flame atomic absorption spectrometry (FAAS), atomic absorption spectrometry and inductively coupled plasma optical emission spectrometer, but they have complicated operations and require pretreatment of the sample prior to determination . On the other hand, colorimetric chemosensors have great advantages in terms of high sensitivity, rapid detection and no pretreatment . Therefore, it is highly desirable to develop effective colorimetric chemosensors for detecting copper.…”

“…[13][14][15][16][17][18] On the other hand, colorimetric chemosensors have great advantages in terms of high sensitivity, rapid detection and no pretreatment. [19][20][21][22][23][24][25][26][27][28] Therefore, it is highly desirable to develop effective colorimetric chemosensors for detecting copper.…”