Nongenomic Steroid Action: Independent Targeting of a Plasma Membrane Calcium Channel and a Tyrosine Kinase

Mendoza, Carmen; Soler, A

doi:10.1006/bbrc.1995.1690

Cited by 82 publications

(30 citation statements)

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“…In this system, rapid steroid action appears to be mediated by a dual mechanism: direct activation of a calcium channel (presumably a receptor-operated channel, thus not dissimilar from the putative calcium channel involved in rapid aldosterone action) and an independent stimulation of tyrosine kinase (75,120). However, calcium release from intracellular stores may be responsible for enhancing tyrosine kinase.…”

Section: Gonadal Steroidsmentioning

confidence: 95%

Specific, Nongenomic Actions of Steroid Hormones

Wehling

1997

Annu. Rev. Physiol.

622

336

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Traditionally, steroid hormone action has been described as the modulation of nuclear transcription, thus triggering genomic events that are responsible for physiological effects. Despite early observations of rapid steroid effects that were incompatible with this theory, nongenomic steroid action has been widely recognized only recently. Evidence for these rapid effects is available for steroids of all clones and for a multitude of species and tissues. Examples of nongenomic steroid action include rapid aldosterone effects in lymphocytes and vascular smooth muscle cells, vitamin D3 effects in epithelial cells, progesterone action in human sperm, neurosteroid effects on neuronal function, and vascular effects of estrogens. Mechanisms of action are being studied with regard to signal perception and transduction, and researchers have developed a patchy sketch of a membrane receptor-second messenger cascade similar to those involved in catecholamine and peptide hormone action. Many of these effects appear to involve phospholipase C, phosphoinositide turnover, intracellular pH and calcium, protein kinase C, and tyrosine kinases. The physiological and pathophysiological relevance of these effects is unclear, but rapid steroid effects on cardiovascular, central nervous, and reproductive functions may occur in vivo. The cloning of the cDNA for the first membrane receptor for steroids should be achieved in the near future, and the physiological and clinical relevance of these rapid steroid effects can then be established.

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Section: Gonadal Steroidsmentioning

confidence: 95%

Specific, Nongenomic Actions of Steroid Hormones

Wehling

1997

Annu. Rev. Physiol.

622

336

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“…The first involves a membranelocated receptor with an extracellular ligand domain to perceive hormone signals, and an intracellular domain responsible for transducing signal through a protein phosphorylation cascade to mediate some responses [44]. The second pathway involves an intracellular steroid-activated receptor complex to directly regulate the transcription of genes by binding to the promoter [45].…”

Section: Br May Regulate Rav1 Through a Bri1-independent Signal Pathwaymentioning

confidence: 99%

Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development

et al. 2004

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RAV1 is a novel DNA-binding protein with two distinct DNA-binding domains unique in higher plants, but its role in plant growth and development remains unknown. Using cDNA array, we found that transcription of RAV1 is downregulated by epibrassinolide (epiBL) in Arabidopsis suspension cells. RNA gel blot analysis revealed that epiBL-regulated RAV1 transcription involves neither protein phosphorylation/dephosphorylation nor newly synthesized protein, and does not require the functional BRI1, suggesting that this regulation might be through a new BR signaling pathway. Overexpressing RAV1 in Arabidopsis results in a retardation of lateral root and rosette leaf development, and the underexpression causes an earlier flowering phenotype, implying that RAV1 may function as a negative regulatory component of growth and development.

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“…Progesterone and 17ot-hydroxyprogesterone have been identified as responsible for the acrosome reaction-inducing activity of human follicular fluid [13]. That progesterone acts at the sperm plasma membrane level is supported by several authors [44,45], but the occurrence of a GABAAlike receptor does not fully account for the differential requirements for stimulation of Ca 2 + influx and for induction of the acrosome reaction [46,47]. Kholkute et al [48] showed that progesterone induced the acrosome reaction and increased fertilization rate in mice.…”

Section: Discussionmentioning

confidence: 95%

In Vitro Capacitating Effect of Gamma-Aminobutyric Acid in Ram Spermatozoa1

Heras

Valcárcel

Pérez

1997

Biology of Reproduction

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We have evaluated the capacitating effect of gamma-aminobutyric acid (GABA) in ram spermatozoa in vitro, in a chemically defined medium, by means of the chlortetracycline (CTC) binding assay. Semen from adult Australian Merino rams was collected in an artificial vagina; spermatozoa were washed once in modified Biggers, Whitten, and Wittingham medium (m-BWW), without BSA or serum, and incubated in m-BWW alone or in m-BWW containing GABA, GABA agonists, or antagonists for 2 h at 38.5 degrees C under 5% CO2 in air. Samples were taken for assessment of CTC binding pattern or were further incubated for 15 min in the presence of 5 microM calcium ionophore A23187. Acrosomal exocytosis was evaluated by Pisum sativum agglutinin binding. Addition of GABA to the incubation medium resulted in a concentration-dependent increase in the percentage of CTC forms II and III, corresponding to mid-capacitated and capacitated spermatozoa, respectively. The effect was marginally significant at 1 microM and maximal at 20 microM. The action of 20 microM GABA was mimicked by the GABAB-receptor agonist, muscimol, but not by the GABAA-receptor agonist, baclofen, and completely blocked by the GABAA-receptor antagonists, bicuculline and picrotoxin, which lacked effect per se. In a separate set of experiments, incubation of spermatozoa with GABA at a concentration of 1 microM, which was insufficient to stimulate sperm capacitation, together with the neuroactive steroid allopregnanolone (1 microM) provoked a capacitating effect similar to that achieved by 20 microM GABA alone. These results show that GABA has a capacitating action on ram spermatozoa through a GABAA receptor-mediated mechanism.

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Nongenomic Steroid Action: Independent Targeting of a Plasma Membrane Calcium Channel and a Tyrosine Kinase

Cited by 82 publications

References 0 publications

Specific, Nongenomic Actions of Steroid Hormones

Specific, Nongenomic Actions of Steroid Hormones

Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development

In Vitro Capacitating Effect of Gamma-Aminobutyric Acid in Ram Spermatozoa1

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