Noninvasive and continuous recordings of auxin fluxes in intact root apex with a carbon nanotube-modified and self-referencing microelectrode

Mancuso, Stefano; Marras, Anna Maria; Magnus, Volker; Baluška, Frantǐsek

doi:10.1016/j.ab.2005.03.054

Cited by 145 publications

(100 citation statements)

References 42 publications

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“…A platinum microelectrode was used to monitor IAA fluxes in Arabidopsis roots as described previously (11,12,34,35). For measurements, plants were grown in hydroponic cultures and used at 5 days after germination.…”

Section: Methodsmentioning

confidence: 99%

“…Second, we employed an IAA-specific microelectrode that has become a reliable tool for non-invasively recording IAA influxes into the root transition zone (11,12,34,35). IAA influx in this zone is characterized by a distinct peak at 200 m from the root tip and is consistent with the current auxin reflux model (8) and a measure for PAT.…”

Section: Bum Alters Auxin Responses and Levels-nextmentioning

confidence: 99%

See 1 more Smart Citation

Identification of an ABCB/P-glycoprotein-specific Inhibitor of Auxin Transport by Chemical Genomics

Kim

Henrichs

Bailly

et al. 2010

Journal of Biological Chemistry

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112

136

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Section: Methodsmentioning

confidence: 99%

Section: Bum Alters Auxin Responses and Levels-nextmentioning

confidence: 99%

Identification of an ABCB/P-glycoprotein-specific Inhibitor of Auxin Transport by Chemical Genomics

Kim

Henrichs

Bailly

et al. 2010

Journal of Biological Chemistry

Self Cite

112

136

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“…In order to verify these data, a novel self-referencing microelectrode [33] was used. In short, this electrode allows non-invasive and continuous recordings of auxin fluxes in intact root apices and has been shown to be highly selective for IAA.…”

Section: Root Hairs Of Atpgp4 Mutants Are Longer and More Variablementioning

confidence: 99%

“…Recording of root apex auxin fluxes using an IAA-specific microelectrode A platinum microelectrode as a substrate electrode immobilizing multiwalled carbon nanotubes was used to monitor IAA fluxes as described in [33]. For measurements, plants were grown in hydroponic cultures and used 5 dag.…”

Section: 7mentioning

confidence: 99%

MDR‐like ABC transporter AtPGP4 is involved in auxin‐mediated lateral root and root hair development

et al. 2005

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Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin.Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss-of-function plants reveal enhanced lateral root initiation and root hair lengths both known to be under the control of auxin. Further, atpgp4 plants show altered sensitivities toward auxin and the auxin transport inhibitor, NPA. Finally, mutant roots reveal elevated free auxin levels and reduced auxin transport capacities. These results together with yeast growth assays suggest a direct involvement of AtPGP4 in auxin transport processes controlling lateral root and root hair development.

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“…In general, it is currently possible to use the SIET or MIFE system to measure net fluxes of ions (such as H + , Ca 2+ , K + , Na + , Cl − , Mg 2+ , NH 4 + , NO 3 − , Cd 2+ ) as well as gaseous molecules (such as O 2 and NO) and other compounds (indoleacetic acid, IAA) in real time in various biological systems (Pineros et al 1998;McLamore et al 2009;Wu et al 2010;Ramos et al 2009;Mancuso et al 2005;Mancuso et al 2000). To our knowledge, only a Cd 2+ liquid-membrane ion selective microelectrode (ISME) was routinely applied in the root system (Pineros et al 1998;Farrell et al 2005;Li et al 2012), although several other heavy metal ions (e.g.…”

Section: Introductionmentioning

confidence: 99%

Determining the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique

Peijnenburg

et al. 2016

Plant Soil

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Background and aims Measuring specific ion fluxes from different regions of the root under practical physiological conditions is crucial for understanding metal uptake mechanisms by plants. Methods We developed and tested a neutral carrierbased liquid-membrane Pb 2+ and Cu 2+ ion selective microelectrode (ISME) to investigate ion-transport processes along the roots of three common wetland plant species. Results The Pb 2+ and Cu 2+ ISME exhibited a Nernstian response with Pb 2+ and Cu 2+ activities as low as 1.0 nM and 1.0 μM in deionized water and simulated soil solution, respectively. Phragmites australis had a region of Cu 2+ release for approximately the first 200 μm, while it exhibited Pb 2+ and Cd 2+ outward net flux up to the first 500 μm. Although in older sections of the root of Phragmites australis there were areas of influx of Cu 2+ , Pb 2+ and Cd 2+ , the overall influx was much smaller than that of Typha latifolia or Canna indica. Such a reduced uptake and/or an increased efflux of metal ions across the root-cell plasma-membrane might explain the higher resistance of Phragmites australis to metals, at least in part. Conclusions The Pb 2+ and Cu 2+ ISMEs are shown to permit detailed investigation of heavy-metal ion transport in plant roots, especially for plants used for phytoremediation.

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Noninvasive and continuous recordings of auxin fluxes in intact root apex with a carbon nanotube-modified and self-referencing microelectrode

Cited by 145 publications

References 42 publications

Identification of an ABCB/P-glycoprotein-specific Inhibitor of Auxin Transport by Chemical Genomics

Identification of an ABCB/P-glycoprotein-specific Inhibitor of Auxin Transport by Chemical Genomics

MDR‐like ABC transporter AtPGP4 is involved in auxin‐mediated lateral root and root hair development

Determining the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique

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