2016
DOI: 10.5966/sctm.2016-0063
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Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing

Abstract: Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffold of decellularized human pericardium, green fluorescent protein‐labeled porcine adipose tissue‐derived progenitor cells (pATPCs), and a customized‐design electrical impedance spectroscopy (EIS) monitoring system. … Show more

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Cited by 29 publications
(34 citation statements)
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“…Here, we characterized the structure as well as the macro and micromechanical scaffold properties after decellularization and recellularization of two biological scaffolds from myocardial and pericardial tissue. Importantly, no adverse events were associated with pericardium isolation from patients, following a safe cardiac surgery procedure previously reported by our group 13 , 18 , 19 . We also evaluated their proteome composition and the ability of the two engineered grafts (once recellularized with ATMSCs) to restore cardiac function.…”
Section: Discussionmentioning
confidence: 53%
“…Here, we characterized the structure as well as the macro and micromechanical scaffold properties after decellularization and recellularization of two biological scaffolds from myocardial and pericardial tissue. Importantly, no adverse events were associated with pericardium isolation from patients, following a safe cardiac surgery procedure previously reported by our group 13 , 18 , 19 . We also evaluated their proteome composition and the ability of the two engineered grafts (once recellularized with ATMSCs) to restore cardiac function.…”
Section: Discussionmentioning
confidence: 53%
“…To investigate whether this in vitro effect occurred in vivo , we analyzed the presence of infiltrating monocytes and CD73 expression in a swine model of MI locally treated with porcine cATMSCs, previously reported by our group to reduce the amount of infiltrating effector T cells in the infarcted tissue and to ameliorate the regeneration of the myocardium ( 15 , 16 ). In these studies, treated animals vs. controls experimented a significant reduction in infarct size (3.4 ± 0.6 vs. 6.5 ± 1%; p = 0.015) and fibrosis in the infarct scar (collagen I/III ratio; 0.49 ± 0.06 vs. 1.66 ± 0.5; p = 0.019), and improved in cardiac function (left ventricular ejection fraction; 7.5 ± 4.9 vs. 1.4 ± 3.7%; p = 0.038, and stroke volume; 11.5 ± 5.9 vs. 3 ± 4.5 ml; p = 0.019).…”
Section: Resultsmentioning
confidence: 99%
“…Remarkably, we previously demonstrated the use of a scaffold of decellularized human pericardium for the local delivery of porcine cATMSCs to the post-MI injured tissue. MSC treatment attained in vivo attenuation of inflammation (i.e., fewer activated T cells) and promoted the regeneration of the damaged myocardial tissue in post-infarcted pigs ( 16 ). Here, we also show the acquisition of CD73 expression by infiltrated host monocytes in MSC-treated animals, thus establishing an adenosinergic positive loop.…”
Section: Discussionmentioning
confidence: 99%
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