Noninvasive, continuous fluorescence monitoring of bilirubin photodegradation

Perkov, Sergei; Gorin, Dmitry A.

doi:10.1039/d2cp03733e

Phys. Chem. Chem. Phys.

2023

DOI: 10.1039/d2cp03733e

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Noninvasive, continuous fluorescence monitoring of bilirubin photodegradation

Sergei Perkov¹,

Dmitry A. Gorin²

Abstract: Nowadays phototherapy is widely used for treatment of various diseases. However, efficient application of phototherapy requires understanding of light interactions with main endogenous chromophores (e.g. hemoglobin, bilirubin, water) in tissue....

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2024

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Fluorescence of various buried fresh and fresh-frozen-thawed tissue types up until the point of active decay: a human taphonomy study

Charlot,

Gasser,

Oostra

et al. 2024

Int J Legal Med

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Forensic taphonomy is the study of postmortem changes of human remains for the purpose of answering legal investigative questions. Many variables can affect the pattern and rate of decomposition of remains, posing challenges for taphonomic studies and estimation of the postmortem interval. Given the gap in knowledge regarding the suitability of using frozen remains to extrapolate conclusions to fresh material, investigating the effects of freeze-thaw cycles followed by burial on human remains is vital for forensic practice and taphonomic research. This study explored the impact of a freeze-thaw cycle and subsequent burial on human tissue decomposition under semi-controlled field conditions. Fresh and fresh-frozen-thawed hands were buried at the Amsterdam Research Initiative for Sub-surface Taphonomy and Anthropology for 31.7 to 340.4 accumulated degree days. Decomposition was assessed using fluorescence measurements targeting protein and fluorescent oxidation products, and broader excitation-emission matrix measurements in skin, adipose, and muscle tissue. Decomposition trends varied primarily by treatment group: fresh samples generally aligned with expectations that protein levels would decrease over time while fluorescent oxidation products increased, whereas fresh-frozen samples deviated significantly from these expectations. Significant differences were found between protein and fluorescent oxidation products levels of fresh and fresh-frozen tissue at corresponding time points, indicating this method’s potential in determining sample state. However, fluorophore peak monitoring in excitation-emission matrices did not prove useful in establishing decomposition trends or potentially distinguishing between sample states. Despite limitations inherent to pilot and human taphonomy studies, this study clearly demonstrates that differences exist in the decomposition of fresh and fresh-frozen tissue, and that these trends vary slightly by tissue type. We therefore conclude that frozen material cannot be considered a proper substitute for fresh tissue regarding taphonomic processes, and the methods used in this study show promise in being used to differentiate between pre-decomposition treatments.

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Fluorescence of various buried fresh and fresh-frozen-thawed tissue types up until the point of active decay: a human taphonomy study

Charlot,

Gasser,

Oostra

et al. 2024

Int J Legal Med

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show abstract

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Noninvasive, continuous fluorescence monitoring of bilirubin photodegradation

Abstract: Nowadays phototherapy is widely used for treatment of various diseases. However, efficient application of phototherapy requires understanding of light interactions with main endogenous chromophores (e.g. hemoglobin, bilirubin, water) in tissue....

Cited by 1 publication

References 44 publications

Fluorescence of various buried fresh and fresh-frozen-thawed tissue types up until the point of active decay: a human taphonomy study

Fluorescence of various buried fresh and fresh-frozen-thawed tissue types up until the point of active decay: a human taphonomy study

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