Noninvasive control of rhodamine-loaded capsules distribution in vivo

Abstract

Using fluorescence spectroscopy system with the fibre-optical probe, we investigated the dynamics of propagation and circulation in the microcirculatory system of experimental nanocapsules fluorescent-labelled (rhodamine TRITC) nanocapsules. The studies were carried out in clinically healthy Wistar rats. The model animals were divided into control group, and the group received injections of the nanocapsules. The fluorescent measurements conducted transcutaneously on the thigh surface. The administration of the preparation with the rhodamine concentration of 5 mg/kg of animal weight resulted in a twofold increase of fluorescence intensity by reference to the baseline level. As a result of the study, it was concluded that fluorescence spectroscopy can be used for transdermal measurements of the rhodamine-loaded capsules in vivo.Keywords: nanocomposite polymeric capsules, fluorescent labelled particles, rhodamine TRITC, drug delivery, fluorescence spectroscopy, blood microcirculation, optical measurements in vivo 1. * o.a.stelmashuk@gmail.com; phone +7 919 269 79 92; www.bmecenter.ru/en/fluorescence_spectroscopy_in_vivo