Noninvasive evaluation of collagen and hemoglobin contents and scattering property of<i>in vivo</i>keloid scars and normal skin using diffuse reflectance spectroscopy: pilot study

Tseng, Sheng Hao; Hsu, Chao‐Kai; Lee, Julia Yu Yun; Tzeng, Shih Yu; Chen, Wan Rung; Liaw, Yu Kai

doi:10.1117/1.jbo.17.7.077005

Cited by 39 publications

(47 citation statements)

References 44 publications

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“…The oxygenated haemoglobin algorithm estimation might be affected when there is a high concentration of melanin, hence the difference in the results. Also the overall values of oxygenated haemoglobin are on average 20% lower than the one reported by Zonios et al [10] but similar to the one from Tseng et al [51]. The difference may be a result of the interrogation depth limit of our system and requires further investigations.…”

Section: Resultssupporting

confidence: 73%

Skin Parameter Map Retrieval from a Dedicated Multispectral Imaging System Applied to Dermatology/Cosmetology

Jolivot

Benezeth

Marzani

2013

International Journal of Biomedical Imaging

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In vivo quantitative assessment of skin lesions is an important step in the evaluation of skin condition. An objective measurement device can help as a valuable tool for skin analysis. We propose an explorative new multispectral camera specifically developed for dermatology/cosmetology applications. The multispectral imaging system provides images of skin reflectance at different wavebands covering visible and near-infrared domain. It is coupled with a neural network-based algorithm for the reconstruction of reflectance cube of cutaneous data. This cube contains only skin optical reflectance spectrum in each pixel of the bidimensional spatial information. The reflectance cube is analyzed by an algorithm based on a Kubelka-Munk model combined with evolutionary algorithm. The technique allows quantitative measure of cutaneous tissue and retrieves five skin parameter maps: melanin concentration, epidermis/dermis thickness, haemoglobin concentration, and the oxygenated hemoglobin. The results retrieved on healthy participants by the algorithm are in good accordance with the data from the literature. The usefulness of the developed technique was proved during two experiments: a clinical study based on vitiligo and melasma skin lesions and a skin oxygenation experiment (induced ischemia) with healthy participant where normal tissues are recorded at normal state and when temporary ischemia is induced.

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Section: Resultssupporting

confidence: 73%

Skin Parameter Map Retrieval from a Dedicated Multispectral Imaging System Applied to Dermatology/Cosmetology

Jolivot

Benezeth

Marzani

2013

International Journal of Biomedical Imaging

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“…Both are parametrically related following the power-law relationship: CBV = CBF Φ where 0<Φ<0.4 [49,50]. Also, the influence of various absorbing molecules at physiologic concentrations lying with these wavelengths such as melanin, melanosome, collagen, cytochtome-c oxidase, carotenes, etc., is insignificant in comparison to HbO 2 and Hbr [51][52][53][54]. In addition, water, which is significant chromophore in the optical window, has negligible absorption below 700nm compared to hemoglobin [55].…”

Section: Hemodynamics Quantificationmentioning

confidence: 99%

Exploring diazepam’s effect on hemodynamic responses of mouse brain tissue by optical spectroscopic imaging

Abookasis

Shochat

Nesher

et al. 2014

Biomed. Opt. Express

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Abstract:In this study, a simple duel-optical spectroscopic imaging apparatus capable of simultaneously determining relative changes in brain oxy-and deoxy-hemoglobin concentrations was used following administration of the anxiolytic compound diazepam in mice with strong dominant (Dom) and submissive (Sub) behavioral traits. Three month old mice (n = 30) were anesthetized and after 10 min of baseline imaging, diazepam (1.5 mg/kg) was administered and measurements were taken for 80 min. The mouse head was illuminated by white light based LED's and diffused reflected light passing through different channels, consisting of a bandpass filter and a CCD camera, respectively, was collected and analyzed to measure the hemodynamic response. This work's major findings are threefold: first, Dom and Sub animals showed statistically significant differences in hemodynamic response to diazepam administration. Secondly, diazepam was found to more strongly affect the Sub group. Thirdly, different time-series profiles were observed post-injection, which can serve as a possible marker for the groups' differentiation. To the best of our knowledge, this is the first report on the effects of an anxiolytic drug on brain hemodynamic responses in mice using diffused light optical imaging. References and links1. R. C. Kessler, P. Berglund, O. Demler, R. Jin, K. R. Merikangas, and E. E. Walters, "Lifetime prevalence and age-of-onset distributions of DSM-IV disorders in the National Comorbidity Survey Replication," Arch. Gen. Psychiatry 62(6), 593-602 (2005 894-902 (2008). 4. M. T. Berlim, M. P. Fleck, and G. Turecki, "Current trends in the assessment and somatic treatment of resistant/refractory major depression: an overview," Ann. Med. 40(2), 149-159 (2008). 5. C. J. Harmer, G. M. Goodwin, and P. J. Cowen, "Why do antidepressants take so long to work? A cognitive neuropsychological model of antidepressant drug action," Br. Ntziachristos, and B. Chance, "Non-invasive and quantitative near-infrared haemoglobin spectrometry in the piglet brain during hypoxic stress, using a frequency-domain multidistance instrument," Phys. Med. Biol. 46(1), 41-62 (2001).

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“…In recent years, Diffuse Reflectance Spectroscopy (DRS) techniques have been developed rapidly and widely used for many biomedical applications [1][2][3][4]. In a typical DRS configuration, light is injected into the sample under investigation and the diffuse reflectance is noninvasively detected at a distance from the source.…”

Section: Introductionmentioning

confidence: 99%

“…In a typical DRS configuration, light is injected into the sample under investigation and the diffuse reflectance is noninvasively detected at a distance from the source. The optical properties of the sample, including absorption (μ a ) and reduced scattering (μ s ') coefficients, can be deduced from the measured diffuse reflectance, and these parameters can in turn provide ample information on a variety of physiological processes [1,2,[5][6][7]. For most cases, DRS techniques rely on a photon transport model to relate the optical properties of samples to the diffuse reflectance.…”

Section: Introductionmentioning

confidence: 99%

A linear gradient line source facilitates the use of diffusion models with high order approximation for efficient, accurate turbid sample optical properties recovery

Lee¹,

Hung²,

Liao³

et al. 2014

Biomed. Opt. Express

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Abstract:In this paper, we demonstrate that a scanning MEMS mirror can be employed to create a linear gradient line source that is equivalent to a planar source. This light source setup facilitates the use of diffusion models of increased orders of approximation having closed form solution, and thus enhance the efficiency and accuracy in sample optical properties recovery. In addition, compared with a regular planar light source, the linear gradient line source occupies much less source area and has an elevated measurement efficiency. We employed a δ-P 1 diffusion equation with a closed form solution and carried out a phantom study to understand the performance of this new method in determining the absorption and scattering properties of turbid samples. Moreover, our Monte Carlo simulation results indicated that this geometry had probing depths comparable to those of the conventional diffuse reflectance measurement geometry with a source-detector separation of 3 mm. We expect that this new source setup would facilitate the investigating of superficial volumes of turbid samples in the wavelength regions where tissue absorption coefficients are comparable to scattering coefficients.

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Noninvasive evaluation of collagen and hemoglobin contents and scattering property ofin vivokeloid scars and normal skin using diffuse reflectance spectroscopy: pilot study

Cited by 39 publications

References 44 publications

Skin Parameter Map Retrieval from a Dedicated Multispectral Imaging System Applied to Dermatology/Cosmetology

Skin Parameter Map Retrieval from a Dedicated Multispectral Imaging System Applied to Dermatology/Cosmetology

Exploring diazepam’s effect on hemodynamic responses of mouse brain tissue by optical spectroscopic imaging

A linear gradient line source facilitates the use of diffusion models with high order approximation for efficient, accurate turbid sample optical properties recovery

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