Noninvasive Measurement of Rodent Intraocular Pressure with a Rebound Tonometer

Wang, Wan Heng; Millar, J. A.; Pang, Iok Hou; Wax, Martin B.; Clark, Abbot F.

doi:10.1167/iovs.05-0781

Cited by 197 publications

(190 citation statements)

References 23 publications

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“…Additional ligations of ≥1 presumably patent episcleral veins were performed at 24 h, and at 1 or 2 weeks following the initial surgery, if IOP was not elevated at 24 h or did not remain elevated at weekly measurement intervals, or if newly formed episcleral veins became evident. Of note, in this study and in our previous studies employing this same model [9][10][11], very few mice required additional ligation surgery at 24 h or 2 weeks after the initial ligation; rather, in most mice, new veins that became apparent by 1 week were ligated, even if IOP had not fallen below levels measured at 24 h. As described previously by us and others [9][10][11][12], IOP determinations were made between 10.00 am and 2.00 pm in both eyes prior to, and at weekly intervals following, IOP elevation using the TonoLab Rebound Tonometer system (Colonial Medical Supply, Franconia, NH, USA).…”

Section: Experimental Glaucoma Modelsupporting

confidence: 76%

Enhanced Retinal Ganglion Cell Survival in Glaucoma by Hypoxic Postconditioning After Disease Onset

et al. 2015

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The neuroprotective efficacy of adaptive epigenetics, wherein beneficial gene expression changes are induced by nonharmful "conditioning" stimuli, is now well established in several acute, preclinical central nervous system injury models. Recently, in a mouse model of glaucoma, we demonstrated retinal ganglion cell (RGC) protection by repetitively "preconditioning" with hypoxia prior to disease onset, indicating an epigenetic approach may also yield benefits in chronic neurodegenerative disease. Herein, we determined whether presenting the repetitive hypoxic stimulus after disease initiation [repetitive hypoxic "postconditioning" (RHPost)] could afford similar functional and morphologic protection against glaucomatous RGC injury. Chronic elevations in intraocular pressure (IOP) were induced unilaterally in adult male C57BL/6 mice by episcleral vein ligation. Mice were randomized to an RH-Post [1 h of systemic hypoxia (11 % oxygen) every other day, starting 4 days after IOP elevation] or an untreated control group. After 3 weeks of experimental glaucoma, the 21-27 % reduction and 5-25 % prolongation in flash visual-evoked potential amplitudes and latencies, respectively, and the 30 % impairment in visual acuity were robustly improved in RH-Post-treated mice, as was the 17 % loss in RGC soma number and 20 % reduction in axon integrity. These protective effects were observed without RH-Post affecting IOP. The present findings demonstrate that functional and morphologic protection of RGCs can be realized by stimulating epigenetic responses during the early stages of disease, and thus constitute a new conceptual approach to glaucoma therapeutics.

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Section: Experimental Glaucoma Modelsupporting

confidence: 76%

Enhanced Retinal Ganglion Cell Survival in Glaucoma by Hypoxic Postconditioning After Disease Onset

et al. 2015

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“…Savinova et al, 11 Danias et al, 14 and Wang et al 15 all determined the IOP of C57BL/ 6J mice under xylazine/ketamine sedation followed by IOP measurements either by cannulation alone or by simultaneous cannulation and rebound tonometry. The data reported by these groups for the normal IOP in C57BL/6 mice (IOP IN ¼ 13.1 mmHg, 5 IOP R ¼ 9.2 and 9.8 mmHg 14,15 ) suggest that rebound tonometry in mice in vivo may underestimate the IOP.…”

Section: Discussionmentioning

confidence: 93%

“…16 The rebound tonometer used in this study is a relatively new device based on similar physical principles as the vibration tonometer. 22,23 Reports of rebound tonometer use in rats, 15,24 mice, 14,15,25 and human patients 26,27 demonstrated that this type of tonometer is convenient to use and yields reliable data.…”

Section: Discussionmentioning

confidence: 99%

Intraocular pressure measurement in mice: a comparison between Goldmann and rebound tonometry

Kim

Kuehn

Anderson

et al. 2006

Eye

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Purpose The development of mouse models of glaucoma requires methods to accurately measure the intraocular pressure (IOP) in this species. The aim of this study was to compare the accuracy of IOP measurements in mice between modified Goldmann and rebound tonometers. Methods IOP was measured either with a modified Goldmann or a rebound tonometer while simultaneously measuring the IOP using invasive manometry in enucleated eyes and in vivo. The level of IOP was controlled hydrostatically. The agreement and correlation between the IOP determined by invasive manometry and by either noninvasive method was evaluated. In addition, the IOP was determined by both noninvasive methods in a cohort of mice with laser-induced ocular hypertension (OHT), and the agreement and correlation between the two tonometry methods were evaluated. Results Measured IOP by either noninvasive tonometer correlated well with those recorded simultaneously by invasive manometry (r ¼ 0.98 for rebound and r ¼ 0.94 for Goldmann). In mice with OHT, the IOP correlation between rebound and modified Goldmann was moderate (r ¼ 0.71); the IOP measured by modified Goldmann tonometry was consistently higher than that by rebound by approximately 5 mmHg. However, the relative per cent increases in IOP were similar between the two methods. Conclusion Both noninvasive methods of IOP measurements in mice are suitable to detect changes in IOP although rebound tonometry correlated better with the invasive manometry readings. The results suggest that the relative, rather than absolute, IOP offers a more reliable means of correlating findings from studies using different tonometers.

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“…In this study, rebound tonometer was used because it is portable, has a lower platinum, and its impact on the eye is fast and minimal. In addition, previous studies reported that measurement of IOP with this instrument was well tolerated in other species, being quick and inducing minimal stress (WANG et al, 2005). The choice of handheld tonometer used in this study was because it was available in the routine of study group, it has already been validated for horses and it does not require anesthetic drops or constant calibration.…”

Section: Discussionmentioning

confidence: 99%

“…Assessment intraocular pressure is crucial parameter for a complete ophthalmic exam as it contributes to the diagnosis of ocular diseases. Several methods have been described for measuring IOP in animals, including applanation, indentation, and rebound tonometry (GUM et al, 1998;KNOLLINGER et al, 2005;WANG et al, 2005 RUSANEM et al, 2010;PEREIRA et al, 2011;PIGATTO et al, 2011). In animals, both applanation and rebound tonometry are used, and correlation has been revealed between these methods (KNOLLINGER et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Lip twitch restraint on rebound tonometry in horses

et al. 2016

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Noninvasive Measurement of Rodent Intraocular Pressure with a Rebound Tonometer

Abstract: The rebound tonometer was easy to use and accurately measured IOP in rats and mice. This technique, together with advances in genetic and other biological studies in rodents, will be valuable in the further understanding of the etiology and pathology of glaucoma.

Cited by 197 publications

References 23 publications

Enhanced Retinal Ganglion Cell Survival in Glaucoma by Hypoxic Postconditioning After Disease Onset

Enhanced Retinal Ganglion Cell Survival in Glaucoma by Hypoxic Postconditioning After Disease Onset

Intraocular pressure measurement in mice: a comparison between Goldmann and rebound tonometry

Lip twitch restraint on rebound tonometry in horses

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