Noninvasive monitoring of ovarian endocrine activity in the chinchilla (Chinchilla lanigera)

Busso, Juan Manuel; Ponzio, Marina Flavia; Cúneo, Marta Fiol de; Ruiz, Rubén Daniel

doi:10.1016/j.ygcen.2006.09.011

Cited by 22 publications

(19 citation statements)

References 37 publications

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“…All values were aligned to day of parturition and expressed as mean ± standard error of mean (SEM), recognizing the fact that hormonal metabolites appear in urine a few hours later than their presence in circulating blood (Busso et al, 2007). Additionally, each hormonal data point in the graph representing weeks-3 to 3 is the mean of hormonal values from the daily samples obtained for those particular weeks, with the exception of LH, which is presented day by day.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Implementation of non-invasive hormone monitoring techniques has been previously attempted in chinchillas (Busso et al, 2007(Busso et al, , 2005b. In female chinchillas, initial work on steroid hormone assessments focused on the excretion of estradiol and progesterone (Busso et al, 2007). While these measures allowed for the identification of significant reproductive events, the study also demonstrated that circulating E 2 and P 4 are heavily metabolized to conjugated forms prior to excretion predominately via the urinary route (>70%) (Busso et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…In female chinchillas, initial work on steroid hormone assessments focused on the excretion of estradiol and progesterone (Busso et al, 2007). While these measures allowed for the identification of significant reproductive events, the study also demonstrated that circulating E 2 and P 4 are heavily metabolized to conjugated forms prior to excretion predominately via the urinary route (>70%) (Busso et al, 2007). EC and PdG assay validations reported in the present study demonstrate that the polyclonal antisera are immunoreactive with some of the major excretory metabolites of sex steroids in female chinchillas.…”

Section: Discussionmentioning

confidence: 99%

“…The E 2 , P 4 and cortisol assays have been previously validated and described in full by our group (Busso et al, 2007;Ponzio et al, 2004). Validations for EC and PdG EIAs, and LH RIA in chinchilla urine were conducted and reported here by demonstrating:…”

Section: Urinary Steroid Analysismentioning

confidence: 99%

See 4 more Smart Citations

Non-invasive endocrine monitoring of ovarian and adrenal activity in chinchilla (Chinchilla lanigera) females during pregnancy, parturition and early post-partum period

Mastromonaco

Cantarelli

Galeano

et al. 2015

General and Comparative Endocrinology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Urinary Steroid Analysismentioning

confidence: 99%

See 3 more Smart Citations

Non-invasive endocrine monitoring of ovarian and adrenal activity in chinchilla (Chinchilla lanigera) females during pregnancy, parturition and early post-partum period

Mastromonaco

Cantarelli

Galeano

et al. 2015

General and Comparative Endocrinology

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Hematopoietic progenitor constituents and adherent cell progenitor morphology isolated from black‐rumped agouti (Dasyprocta prymnolopha, Wagler 1831) bone marrow

Rocha

Alves

Neto

et al. 2012

Microscopy Res & Technique

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Stem cells are present in the adult tissues of most diverse species. Bone marrow is recognized to be the most exploited site to obtain stem cells and cell progenitors. The objective of the present study was to characterize hematopoietic progenitor (HP) morphology and analyze the performance of adherent cell progenitors (ACPs) cultivated in vitro from black-rumped agouti bone marrow (Dasyprocta prymnolopha). Bone marrow aspirates were obtained from tibia crest and used to prepare histological slides and identify cell morphology. Cells were also scattered on culture plates for later isolation, expansion, and quantification. Smears obtained from bone marrow demonstrated HPs at different stages of maturity. In culture, these cells showed fibroblastoid morphology and a strong tendency to form colonies, demonstrated by the presence of cell aggregates, cytoplasmic elongations lying side by side. An 80% cell confluence was observed at 18 days in culture and progressive reduction in the percentage of nonadherent mononuclear cells. After eight passes, a mean cell viability of 96.07% was observed, from a pool of 1.6 × 10(7) cells (ACP). Thirteen 25-cm(2) culture bottles were trypsinized, resuspended in freezing medium, stored in 14 criotubes at a concentration of 1 × 10(6) cells per milliliter, and placed in liquid nitrogen at -196°C. Agouti bone marrow demonstrated high plasticity, moreover different HP lines, and a population of adherent cells demonstrated morphology similar to mesenchymal stem cells in culture.

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Distribution and concentration of maternal progesterone in the yolk of Greater Rhea eggs (Rhea americana)

et al. 2017

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Progesterone is the most concentrated maternal yolk steroid characterized to date in birds; however, no information about it is available in ratite eggs. We collected freshly laid eggs from zoo-housed Greater Rhea females (Rhea americana) bred under similar rearing conditions during two breeding seasons to characterize concentration and distribution of maternal yolk progesterone. After high-performance liquid chromatography analysis, yolk hormone was measured using a commercial electrochemiluminescence immunoassay. Progesterone concentrations were found to vary significantly among the yolk layers, supporting a follicular origin for this steroid in Greater Rhea eggs. Additionally, highly similar mean absolute yolk progesterone concentrations were detected between 2013 and 2015 breeding seasons (1,332.98 ± 82.59 and 1,313.59 ± 85.19 ng/g, respectively). These values are also comparable to those found in some domestic carinate species. Findings suggest that at population level, when rearing conditions are similar, mean absolute yolk maternal progesterone concentrations also appear bounded. Future research on the factors and mechanisms that regulate progesterone deposition in Greater Rhea eggs is needed to better understand whether its levels depend on different rearing conditions.

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Noninvasive monitoring of ovarian endocrine activity in the chinchilla (Chinchilla lanigera)

Cited by 22 publications

References 37 publications

Non-invasive endocrine monitoring of ovarian and adrenal activity in chinchilla (Chinchilla lanigera) females during pregnancy, parturition and early post-partum period

Non-invasive endocrine monitoring of ovarian and adrenal activity in chinchilla (Chinchilla lanigera) females during pregnancy, parturition and early post-partum period

Hematopoietic progenitor constituents and adherent cell progenitor morphology isolated from black‐rumped agouti (Dasyprocta prymnolopha, Wagler 1831) bone marrow

Distribution and concentration of maternal progesterone in the yolk of Greater Rhea eggs (Rhea americana)

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