Noninvasive phenotypic analysis of cardiovascular structure and function in fetal mice using ultrasound

Leatherbury, Linda; Yu, Qing; Lo, Cecilia

doi:10.1002/bdrc.10005

Cited by 19 publications

(18 citation statements)

References 14 publications

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“…Ultrasound imaging is now commonly used for in vivo mouse phenotyping, including the noninvasive interrogation of fetuses in utero (Leatherbury et al, 2003;Yu et al, 2008). This imaging modality is well developed for clinical applications.…”

Section: High-resolution Ultrasound Biomicroscopymentioning

confidence: 99%

Imaging modalities to assess structural birth defects in mutant mouse models

Tobita

Liu

2010

Birth Defects Research Pt C

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Assessment of structural birth defects (SBDs) in animal models usually entails conducting detailed necropsy for anatomical defects followed by histological analysis for tissue defects. Recent advances in new imaging technologies have provided the means for rapid phenotyping of SBDs, such as using ultra-high frequency ultrasound biomicroscopy, optical coherence tomography, micro-CT, and micro-MRI. These imaging modalities allow the detailed assessment of organ/tissue structure, and with ultrasound biomicroscopy, structure and function of the cardiovascular system also can be assessed noninvasively, allowing the longitudinal tracking of the fetus in utero. In this review, we briefly discuss the application of these state-of-the-art imaging technologies for phenotyping of SBDs in rodent embryos and fetuses, showing how these imaging modalities may be used for the detection of a wide variety of SBDs.

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Section: High-resolution Ultrasound Biomicroscopymentioning

confidence: 99%

Imaging modalities to assess structural birth defects in mutant mouse models

Tobita

Liu

2010

Birth Defects Research Pt C

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“…For example, it might be possible to visualize the fetal heart and perform functional cardiac imaging in prenatal studies on congenital cardiac malformation in transgenic mice. 23,24 Furthermore, gating can still be performed even when the animal is kept in a sterile container for infection control as long as the container is x-ray lucent.…”

Section: Improved Animal Handlingmentioning

confidence: 99%

Intrinsic Gating for Small-Animal Computed Tomography

Dinkel

Bartling

Kuntz

et al. 2008

Circ: Cardiovascular Imaging

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Background-A projection-based method of intrinsic cardiac gating in small-animal computed tomography imaging is presented. Methods and Results-In this method, which operates without external ECG monitoring, the gating reference signal is derived from the raw data of the computed tomography projections. After filtering, the derived gating reference signal is used to rearrange the projection images retrospectively into data sets representing different time points in the cardiac cycle during expiration. These time-stamped projection images are then used for tomographic reconstruction of different phases of the cardiac cycle. Intrinsic gating was evaluated in mice and rats and compared with extrinsic retrospective gating. An excellent agreement was achieved between ECG-derived gating signal and self-gating signal (coverage probability for a difference between the 2 measurements to be less than 5 ms was 89.2% in mice and 85.9% in rats). Functional parameters (ventricular volumes and ejection fraction) obtained from the intrinsic and the extrinsic data sets were not significantly different. The ease of use and reliability of intrinsic gating were demonstrated via a chemical stress test on 2 mice, in which the system performed flawlessly despite an increased heart rate. Because of intrinsic gating, the image quality was improved to the extent that even the coronary arteries of mice could be visualized in vivo despite a heart rate approaching 430 bpm. Feasibility of intrinsic gating for functional imaging and assessment of cardiac wall motion abnormalities was successfully tested in a mouse model of myocardial infarction. Conclusions-Our results demonstrate that self-gating using advanced software postprocessing of projection data promises to be a valuable tool for rodent computed tomography imaging and renders ECG gating with external electrodes superfluous. (Circ Cardiovasc Imaging. 2008;1:235-243.)

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“…However, real-time measurements are critical for characterization of in vivo function, while clinical applications of MRI during pregnancy are limited due to instrumentation cost and the inability to use the system at bench-side. Ultrasound provides high spatial resolution for structural phenotyping [5][6][7][8][9][10][11] and has provided new insights on the functional effects on the heart of teratogen exposure [12]. Photoacoustic imaging-using a short laser pulse to generate ultrasound transients from optical absorbers-improves image contrast while maintaining many advantages of ultrasound imaging.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-guided spectral photoacoustic imaging of hemoglobin oxygenation during development

Bayer

Wlodarczyk

Finnell

et al. 2017

Biomed. Opt. Express

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Few technologies are capable of imaging in vivo function during development. In this study, we have implemented spectral photoacoustic imaging to estimate tissue oxygenation longitudinally in pregnant mice. We used the spectral photoacoustic signal to estimate hemoglobin oxygen saturation within intact, in vivo mouse concepti from developmental day (E) 8.5 to E16.5-a first step towards functional imaging of the maternalfetal environment. Future work will apply these methods to compare longitudinal functional changes during normal vs abnormal development of embryos, fetuses, and placentas. 422-437 (2012). 14. J. Weber, P. C. Beard, and S. E. Bohndiek, "Contrast agents for molecular photoacoustic imaging," Nat.Methods 13 Physiol. 46(3), 599-602 (1979). 32. R. Serianni, J. Barash, T. Bentley, P. Sharma, J. L. Fontana, D. Via, J. Duhm, R. Bunger, and P. D. Mongan, "Porcine-specific hemoglobin saturation measurements," J. Appl. Physiol. 94, 561-566 (2003).

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Noninvasive phenotypic analysis of cardiovascular structure and function in fetal mice using ultrasound

Cited by 19 publications

References 14 publications

Imaging modalities to assess structural birth defects in mutant mouse models

Imaging modalities to assess structural birth defects in mutant mouse models

Intrinsic Gating for Small-Animal Computed Tomography

Ultrasound-guided spectral photoacoustic imaging of hemoglobin oxygenation during development

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