Noninvasive Quantification of Tumor Volume in Preclinical Liver Metastasis Models Using Contrast-Enhanced X-Ray Computed Tomography

Graham, Kevin C.; Ford, Nancy L.; MacKenzie, Lisa T.; Postenka, Carl O.; Groom, A. C.; Macdonald, Ian; Holdsworth, David W.; Drangova, Maria; Chambers, Ann F.

doi:10.1097/rli.0b013e31815603d7

Cited by 37 publications

(38 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The advantages and limitations of these modalities, including positron emission tomography, single-photon emission computed tomography, computed tomography, magnetic resonance (MR), ultrasound, and bioluminescent and fluorescent optical imaging, are well documented (9)(10)(11)(12)(13)(14). To date, the majority of studies focusing specifically on solitary metastatic cells have relied on fluorescent optical imaging due primarily to the high-resolution capabilities of this modality.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

et al. 2009

Self Cite

View full text Add to dashboard Cite

The metastatic cell population, ranging from solitary cells to actively growing metastases, is heterogeneous and unlikely to respond uniformly to treatment. However, quantification of the entire experimental metastatic cell population in whole organs is complicated by requirements of an imaging modality with the large field of view and high spatial resolution necessary to detect both single cells and metastases in the same organ. Thus, it is difficult to assess differential responses of these distinct metastatic populations to therapy. Here, we develop a magnetic resonance imaging (MRI) technique capable of quantifying the full population of metastatic cells in a secondary organ. B16F1 mouse melanoma cells were labeled with micron-sized iron oxide particles (MPIO) and injected into mouse liver via the mesenteric vein. Livers were removed immediately or at day 9 or 11, following doxorubicin or vehicle control treatment, and imaged using a 3T clinical magnetic resonance scanner and custom-built gradient coil. Both metastases (>200 μm) and MPIO-labeled single cells were detected and quantified from MR images as areas of hyperintensity or hypointensity (signal voids), respectively. We found that 1 mg/kg doxorubicin treatment inhibited metastasis growth (n = 11 per group; P = 0.02, t test) but did not decrease the solitary metastatic cell population in the same livers (P > 0.05). Thus, the technique presented here is capable of quickly quantifying the majority of the metastatic cell population, including both growing metastases and solitary cells, in whole liver by MRI and can identify differential responses of growing metastases and solitary cells to therapy. [Cancer Res 2009;69(21):8326-31]

show abstract

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…Micro-CT has proven to be a useful tool for pre-clinical anatomic assessment of small animal models including cardiovascular system (Jorgensen et al 1998;Langheinrich et al 2004;Schambach et al 2010;Badea et al 2005), gastrointestinal system (Graham et al 2008;Martiniova et al 2010) and skeletal system (Dom et al 2012;Jiang et al 2000). Micro-CT can differentiate between bone and soft tissues and provide high resolution images for both cortical and cancellous bones (Jayusman 2015).…”

Section: Discussionmentioning

confidence: 99%

Effects of Eurycoma Longifolia on Fracture Healing of Androgen-Deficient Osteoporosis Model: A Micro Computed Tomograph Analysis

Shuid¹

2016

View full text Add to dashboard Cite

ABSTRAK Tomografi berkomputer mikro (mikro-CT) adalah peralatan yang berguna untuk memantau pemulihan patah tulang pada model osteoporosis. Ia menyediakan imej 3D kalus fraktur yang boleh digunakan untuk mengira parameter tulang secara kuantitatif. Dalam kajian in, mikro & Health 2016;11(2): 267-277 Azri J. et al. osteoporotic fracture in males was associated with significant morbidity and has huge impact emotionally and financially on their families and the society (Johnell et al. 2001).There are three phases of fracture healing; the reactive phase, reparative phase and remodeling phase. In the reactive phase, platelets are activated to release products such as transforming growth factor-β that triggers inflammation. At the same time, haematoma is formed to seal off the fracture area (Grundnes & Reikeras 1993;Marsh & Li 1999). In the reparative phase, granulation tissue is replaced by fibrocartilage and external callus is formed by intramembranous ossification at the periosteum. Finally, in the remodeling phase, the callus and ABSTRACTMicro computed tomography (micro-CT) imaging is a useful tool to monitor fracture healing in osteoporosis model. It creates a 3-D image of the fracture callus which can be analysed to assess bone parameters quantitatively. In this study, micro-CT was used to assess the fracture healing of orchidectomised rats, an androgen-deficient osteoporosis model. The effects of Eurycoma longifolia, a medicinal plant with proandrogenic effects, on fracture healing were assessed. The rats were grouped into orchidectomised-control (ORX), sham-operated (SHAM), orchidectomised and injected with testosterone intramuscularly once weekly (TEN) and orchidectomised and daily oral gavage of Eurycoma longifolia (EL). Treatment duration was six weeks following bone fracture. Fracture was induced in the right tibia of all the rats. A total of 100 axial slices above and below fracture line were scanned with a micro-CT. The micro-CT analysis was able to detect significant difference in the fracture healing rate of ORX and TEN groups. The bridging cortices and fraction of mineralized tissue of the bridging cortices of the callous of ORX group was significantly lower than TEN group. No significant micro-CT changes were seen in the fracture healing of the EL group. The effect of EL on fracture healing was not demonstrable in orchidectomised rat model.

show abstract

“…Because many liver metastases grow on the surface of the liver, a combined strategy of Fenestra VC plus an intraperitoneal injection of extracellular contrast agent was shown to improve the precision and accuracy of tumor volume measurement and enables the quantitation of total tumor burden (Graham et al, 2008). Small lesions like liver metastases have also been imaged using a nanoparticle alkaline earth metal-based contrast agent (Viscover ExiTron nano) developed for small-animal liver CT imaging (Boll et al, 2011).…”

Section: Mgmentioning

confidence: 99%

“…Liver tumor volumes have also been quantified in vivo using Fenestra VC mice injected with murine B16F1 melanoma cells; in this case, Fenestra VC was used instead of Fenestra LC in order to remove the ambiguity between unopacified liver vessels and tumors in the liver by selecting a time point postcontrast injection (8 h) (Graham et al, 2008). Because many liver metastases grow on the surface of the liver, a combined strategy of Fenestra VC plus an intraperitoneal injection of extracellular contrast agent was shown to improve the precision and accuracy of tumor volume measurement and enables the quantitation of total tumor burden (Graham et al, 2008).…”

Section: Mgmentioning

confidence: 99%

High-Resolution CT for Small-Animal Imaging Research

Badea

Panetta²

2014

Comprehensive Biomedical Physics

View full text Add to dashboard Cite

Noninvasive Quantification of Tumor Volume in Preclinical Liver Metastasis Models Using Contrast-Enhanced X-Ray Computed Tomography

Abstract: Imaging at a time period of equal liver parenchyma and vascular enhancement after contrast injection allows for clear delineation of liver-tumor borders, thereby enabling quantitative tumor-volume monitoring.

Cited by 37 publications

References 29 publications

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

Effects of Eurycoma Longifolia on Fracture Healing of Androgen-Deficient Osteoporosis Model: A Micro Computed Tomograph Analysis

High-Resolution CT for Small-Animal Imaging Research

Contact Info

Product

Resources

About